CSK antibody
Goat anti Human CSK antibody recognizes an epitope within the C-terminal (CT) region of CSK, also known as c-src tyrosine kinase, Tyrosine-protein kinase CSK, p50CSK or Protein-tyrosine kinase CYL. CSK is a cytoplasmic tyrosine kinase ubiquitously expressed as two mRNA species (Partanen et al. 1991), which down-regulates the activity of the c-src oncoprotein (Chong et al. 2005).
CSK is expressed in lung tissues and by macrophages and is highly conserved across species suggesting a role in important regulatory functions (Bräuninger et al. 1992).
CSK is expressed in lung tissues and by macrophages and is highly conserved across species suggesting a role in important regulatory functions (Bräuninger et al. 1992).
Product Details
- Target Species
- Human
- Species Cross-Reactivity
-
Target Species Cross Reactivity Rat Mouse - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Antiserum Preparation
- Antisera to human CSK (CT) were raised by repeated immunisations of goats with highly purified antigen. Purified IgG was prepared by affinity chromatography.
- Buffer Solution
- TRIS buffered saline
- Preservative Stabilisers
- <0.1% Sodium Azide (NaN3)
- Immunogen
- Peptide sequence EQLEHIKTHELHL corresponding to the C-terminal region of CSK (NP_004374.1).
- Approx. Protein Concentrations
- IgG concentration 0.5 mg/ml
Storage Information
- Storage
- This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. - Guarantee
- 12 months from date of despatch
More Information
- UniProt
- P41240
- Entrez Gene
- CSK
- GO Terms
- GO:0007596 blood coagulation
- GO:0005829 cytosol
- GO:0004715 non-membrane spanning protein tyrosine kinase activity
- GO:0005524 ATP binding
- GO:0005794 Golgi apparatus
- GO:0005813 centrosome
- GO:0006468 protein phosphorylation
- GO:0007173 epidermal growth factor receptor signaling pathway
- GO:0008022 protein C-terminus binding
- GO:0031295 T cell costimulation
- GO:0050852 T cell receptor signaling pathway
- Regulatory
- For research purposes only
Applications of CSK antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA | >1/32,000 | ||
| Immunohistology - Paraffin 1 | 0.1ug/ml | 0.3ug/ml | |
| Western Blotting | 0.5ug/ml | 2.0ug/ml |
- 1This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.
TRIS/EDTA buffer pH 9.0 is recommended for this purpose.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
- Histology Positive Control Tissue
- Human Tonsil
- Western Blotting
- AHP733 detects a band of approximately 50kDa in Jurkat cell lysates.
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Secondary Antibodies Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| Rabbit anti Goat IgG (Fc):FITC | STAR122F | F | 1 mg |
|
|
| Rabbit anti Goat IgG (Fc):HRP | STAR122P | C E WB | 1 mg |
|
Useful Reagents Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| Antigen Retrieval Buffer, pH8.0 | BUF025A | P | 500 ml | ||
| 100x Antigen Retrieval Buffer, pH8.0 | BUF025C | P | 50 ml |
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