CSK antibody

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Goat anti Human CSK (C-Terminal)

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
AHP733 E P* WB 0.1 mg
Goat anti Human CSK antibody recognizes an epitope within the C-terminal (CT) region of CSK, also known as c-src tyrosine kinase, Tyrosine-protein kinase CSK, p50CSK or Protein-tyrosine kinase CYL. CSK is a cytoplasmic tyrosine kinase ubiquitously expressed as two mRNA species (Partanen et al. 1991), which down-regulates the activity of the c-src oncoprotein (Chong et al. 2005).

CSK is expressed in lung tissues and by macrophages and is highly conserved across species suggesting a role in important regulatory functions (Bräuninger et al. 1992).

Product Details

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Rat
Mouse
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Antiserum Preparation
Antisera to human CSK (CT) were raised by repeated immunisations of goats with highly purified antigen. Purified IgG was prepared by affinity chromatography.
Buffer Solution
TRIS buffered saline
Preservative Stabilisers
0.02%Sodium Azide
0.5%Bovine Serum Albumin
Immunogen
Peptide sequence EQLEHIKTHELHL corresponding to the C-terminal region of CSK (NP_004374.1).
Approx. Protein Concentrations
IgG concentration 0.5 mg/ml

Storage Information

Storage
Store at +4oC or at -20oC if preferred.

This product should be stored undiluted.

Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.

More Information

UniProt
P41240 Related reagents
Entrez Gene
CSK Related reagents
GO Terms
GO:0007596 blood coagulation
GO:0005829 cytosol
GO:0004715 non-membrane spanning protein tyrosine kinase activity
GO:0005524 ATP binding
GO:0005794 Golgi apparatus
GO:0005813 centrosome
GO:0006468 protein phosphorylation
GO:0007173 epidermal growth factor receptor signaling pathway
GO:0008022 protein C-terminus binding
GO:0031295 T cell costimulation
GO:0050852 T cell receptor signaling pathway
Regulatory
For research purposes only

Applications of CSK antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA >1/32,000
Immunohistology - Paraffin 1 0.1ug/ml 0.3ug/ml
Western Blotting 0.5ug/ml 2.0ug/ml
  1. 1This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.
    TRIS/EDTA buffer pH 9.0 is recommended for this purpose.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.

Histology Positive Control Tissue
Human Tonsil
Western Blotting
AHP733 detects a band of approximately 50kDa in Jurkat cell lysates.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Rabbit anti Goat IgG (Fc):FITC STAR122F 1 mg F
Rabbit anti Goat IgG (Fc):HRP STAR122P 1 mg C E WB

Useful Reagents Available

Description Product Code Pack Size Applications List Price Quantity
Antigen Retrieval Buffer, pH8.0 BUF025A 500 ml P
Antigen Retrieval Buffer, pH8.0 BUF025C 50 ml P
Histar Detection System STAR3000B 150 Tests C P