CSK antibody

Goat anti Human CSK (C-Terminal)

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Specificity
CSK
Region
(C-TERMINAL)

Product Code Applications Pack Size List Price Your Price Qty
AHP733
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
E P * WB 0.1 mg
List Price Your Price
Search for Batch Specific Datasheets

Goat anti Human CSK antibody recognizes an epitope within the C-terminal (CT) region of CSK, also known as c-src tyrosine kinase, Tyrosine-protein kinase CSK, p50CSK or Protein-tyrosine kinase CYL. CSK is a cytoplasmic tyrosine kinase ubiquitously expressed as two mRNA species (Partanen et al. 1991), which down-regulates the activity of the c-src oncoprotein (Chong et al. 2005).

CSK is expressed in lung tissues and by macrophages and is highly conserved across species suggesting a role in important regulatory functions (Bräuninger et al. 1992).

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Rat
Mouse
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Antiserum Preparation
Antisera to human CSK (CT) were raised by repeated immunisations of goats with highly purified antigen. Purified IgG was prepared by affinity chromatography.
Buffer Solution
TRIS buffered saline
Preservative Stabilisers
<0.1%Sodium Azide (NaN3)
0.5%Bovine Serum Albumin
Immunogen
Peptide sequence EQLEHIKTHELHL corresponding to the C-terminal region of CSK (NP_004374.1).
Approx. Protein Concentrations
IgG concentration 0.5 mg/ml
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA >1/32,000
Immunohistology - Paraffin 1 0.1ug/ml 0.3ug/ml
Western Blotting 0.5ug/ml 2.0ug/ml
  1. 1This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.
    TRIS/EDTA buffer pH 9.0 is recommended for this purpose.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.

Histology Positive Control Tissue
Human Tonsil
Western Blotting
AHP733 detects a band of approximately 50kDa in Jurkat cell lysates.

Description Product Code Applications Pack Size List Price Your Price Quantity
Rabbit anti Goat IgG (Fc):HRP STAR122P C E WB 1 mg loader
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loader
Description Rabbit anti Goat IgG (Fc):HRP
Rabbit anti Goat IgG (Fc):FITC STAR122F F 1 mg loader
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loader
Description Rabbit anti Goat IgG (Fc):FITC

Description Product Code Applications Pack Size List Price Your Price Quantity
Antigen Retrieval Buffer, pH8.0 BUF025A P 500 ml
List Price Your Price
Description Antigen Retrieval Buffer, pH8.0

RRID
AB_2229819
UniProt
P41240
Entrez Gene
CSK
GO Terms
GO:0007596 blood coagulation
GO:0005829 cytosol
GO:0004715 non-membrane spanning protein tyrosine kinase activity
GO:0005524 ATP binding
GO:0005794 Golgi apparatus
GO:0005813 centrosome
GO:0006468 protein phosphorylation
GO:0007173 epidermal growth factor receptor signaling pathway
GO:0008022 protein C-terminus binding
GO:0031295 T cell costimulation
GO:0050852 T cell receptor signaling pathway
Request a different product with this specificity

Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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