CRTC2 antibody

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Goat anti CRTC2

CETC2 antibody recognizes CREB-regulated transcription coactivator 2, also known as transducer of regulated cAMP response element-binding protein 2, TORC-2 or transducer of CREB protein 2. The antibody has been extensively validated for western blotting using whole cell lysates.

CRTC2 Antibody with Control Lysate

CETC2 antibody recognizes CREB-regulated transcription coactivator 2, also known as transducer of regulated cAMP response element-binding protein 2, TORC-2 or transducer of CREB protein 2. The antibody has been extensively validated for western blotting using whole cell lysates.

Product Type
PrecisionAb™ Polyclonal
Isotype
Polyclonal IgG
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
VPA00131 WB 100 µl
VPA00131KT WB 2 Westerns
With Lysate 400µg
Goat anti Human CRTC2 antibody recognizes the CREB-regulated transcription coactivator 2, also known as transducer of regulated cAMP response element-binding protein 2 (TORC-2) or transducer of CREB protein 2.

CRTC2 is a member of the transducers of regulated cAMP response element-binding protein activity family of transcription coactivators. These proteins promote the transcription of genes targeted by the cAMP response element-binding protein, and therefore play an important role in many cellular processes. Under basal conditions CRTC2 is phosphorylated by AMP-activated protein kinase or the salt-inducible kinases and is sequestered in the cytoplasm. Upon activation by elevated cAMP or calcium, CRTC2 translocates to the nucleus and increases target gene expression. Single nucleotide polymorphisms in the CRTC2 gene may increase the risk of type 2 diabetes. A pseudogene of CRTC2 is located on the long arm of chromosome 5 (provided by RefSeq, Dec 2010).

Goat anti Human CRTC2 antibody detects a band of 80 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.

Product Details

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse
Rat
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Product Form
Purified IgG - liquid
Preparation
Goat polyclonal antibody purified by affinity chromatography
Preparation
20μl Goat polyclonal antibody purified by affinity chromatography
Buffer Solution
TRIS buffered saline
Buffer Solution
TRIS buffered saline
Preservative Stabilisers
0.02% Sodium Azide (NaN3) 0.5 % BSA
Preservative Stabilisers
0.02% Sodium Azide (NaN3) 0.5 % BSA
Immunogen
Peptide with the sequence C-DPAVEDSFRSDRLQ, from the C Terminus of the protein sequence

Storage Information

Storage
Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
Storage
Antibody: Store undiluted at -20oC, avoiding repeated freeze thaw cycles.

Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage.
Shelf Life
As supplied, 12 months from date of despatch
Shelf Life
As supplied, 12 months from date of despatch.

More Information

UniProt
Q53ET0 Related reagents
Entrez Gene
CRTC2 Related reagents
GO Terms
GO:0005515 protein binding
GO:0005634 nucleus
GO:0005737 cytoplasm
GO:0006350 transcription
GO:0044419 interspecies interaction between organisms
GO:0045449 regulation of transcription
Acknowledgements
PrecisionAb™ is a trademark of Bio-Rad Laboratories.
Acknowledgements
PrecisionAb™ is a trademark of Bio-Rad Laboratories.
Regulatory
For research purposes only

Applications of CRTC2 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000
Western Blotting 1/1000

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
Western Blotting
Anti CRTC2 detects a band of approximately 80 kDa in MCF7 cell lysates.
Western Blotting
Anti CRTC2 detects a band of approximately 80 kDa in MCF7 cell lysates.
Instructions For Use
Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.
Instructions For Use
Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Donkey anti Sheep/Goat IgG:HRP STAR88P 1 ml C E P WB
Donkey anti Sheep/Goat IgG:HRP STAR88P 1 ml C E P WB