The CRKL gene encodes a protein kinase containing SH2 and SH3 (src homology) domains which has been shown to activate the RAS and JUN kinase signaling pathways and transform fibroblasts in a RAS-dependent fashion (Senechal et al. 1996). It is a substrate of the BCR-ABL tyrosine kinase, plays a role in fibroblast transformation by BCR-ABL, and may be oncogenic (provided by RefSeq, Jan 2009).
Rabbit anti Human CRKL antibody detects a band of 39 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.
- Target Species
- Western Blotting
- Anti CRKL detects a band of approximately 39 kDa in K562 cell lysates.
- Product Form
- Purified IgG - liquid.
- Rabbit polyclonal antibody purified by affinity chromatography.
- Buffer Solution
- Phosphate buffered saline.
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
- Synthetic peptide directed towards the middle region of human CRKL
- Store undiluted at -20°C, avoiding repeated freeze thaw cycles.
- 12 months from date of despatch
- Entrez Gene
- GO Terms
- GO:0005829 cytosol
- GO:0004713 protein tyrosine kinase activity
- GO:0004871 signal transducer activity
- GO:0005070 SH3/SH2 adaptor activity
- GO:0007254 JNK cascade
- GO:0007265 Ras protein signal transduction
- PrecisionAb is a trademark of Bio-Rad Laboratories
- For research purposes only.
Applications of CRKL antibody
|Application Name||Verified||Min Dilution||Max Dilution|
The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.
Secondary Antibodies Available
|Description||Product Code||Applications||Pack Size||List Price||Quantity|
|Goat anti Rabbit IgG (H/L):HRP||STAR208P||WB||2 ml|