CRKL antibody

Goat anti CRKL

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Specificity
CRKL

Product Code Applications Pack Size List Price Your Price Qty
AHP693
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
WB 0.1 mg
List Price Your Price
Search for Batch Specific Datasheets

Goat anti Human CRKL antibody recognizes CRKL, a ~33 kDa adaptor protein shown to activate the RAS and JUN kinase signalling pathways and transform fibroblasts in a RAS-dependant manner.

CRKL is a substitute of BCR-ABL tyrosine kinase.

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Antiserum Preparation
Antisera to CRKL were raised by repeated immunisations of goats with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
Buffer Solution
TRIS buffered saline
Preservative Stabilisers
<0.1% Sodium Azide (NaN3)
0.5% Bovine Serum Albumin
Immunogen
Synthetic peptide sequence KIFDPQNPDENE derived from C-terminus of CRKL.
Approx. Protein Concentrations
IgG concentration 0.5 mg/ml
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/200 1/500
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Western Blotting
AHP693 detects a band of approximately 33kDa in mouse kidney cell lysates. Overnight incubation with primary antibody is recommended.

Description Product Code Applications Pack Size List Price Your Price Quantity
Rabbit anti Goat IgG (Fc):HRP STAR122P C E WB 1 mg loader
List Price Your Price
loader
Description Rabbit anti Goat IgG (Fc):HRP
Rabbit anti Goat IgG (Fc):FITC STAR122F F 1 mg loader
List Price Your Price
loader
Description Rabbit anti Goat IgG (Fc):FITC

Further Reading

  1. Senechal, K. et al. (1996) The CRKL adaptor protein transforms fibroblasts and functions in transformation by the BCR-ABL oncogene.
    J Biol Chem. 271 (38): 23255-61.

RRID
AB_2085056
UniProt
P46109
Entrez Gene
CRKL
GO Terms
GO:0005829 cytosol
GO:0004713 protein tyrosine kinase activity
GO:0004871 signal transducer activity
GO:0005070 SH3/SH2 adaptor activity
GO:0007254 JNK cascade
GO:0007265 Ras protein signal transduction
View more products with CRKL specificity

Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

View all Anti-Human Products