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CRKL antibody

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Anti CRKL Antibody gallery image 1
Western blotting analysis of K562 lysate (35µg protein in RIPA buffer) probed with Goat anti Human CRKL antibody (AHP693) at 0.01μgml-1. Primary incubation was 1 hour. Signal was detected by chemiluminescence

Goat anti CRKL

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Product Code Applications Pack Size List Price Quantity
AHP693
Datasheet
WB
MSDS
0.1 mg

Goat anti Human CRKL antibody recognizes CRKL, a ~33 kDa adaptor protein shown to activate the RAS and JUN kinase signalling pathways and transform fibroblasts in a RAS-dependant manner.

CRKL is a substitute of BCR-ABL tyrosine kinase.

Product Details

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Antiserum Preparation
Antisera to CRKL were raised by repeated immunisations of goats with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
Buffer Solution
TRIS buffered saline
Preservative Stabilisers
0.02%Sodium Azide
0.5%Bovine Serum Albumin
Immunogen
Synthetic peptide sequence KIFDPQNPDENE derived from C-terminus of CRKL.
Approx. Protein Concentrations
IgG concentration 0.5 mg/ml

Storage Information

Storage
Store at +4oC or at -20oC if preferred.

This product should be stored undiluted.

Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Guarantee
18 months from date of despatch.

More Information

UniProt
P46109 Related reagents
Entrez Gene
CRKL Related reagents
GO Terms
GO:0005829 cytosol
GO:0004713 protein tyrosine kinase activity
GO:0004871 signal transducer activity
GO:0005070 SH3/SH2 adaptor activity
GO:0007254 JNK cascade
GO:0007265 Ras protein signal transduction
Regulatory
For research purposes only

Applications of CRKL antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/200 1/500
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Western Blotting
AHP693 detects a band of approximately 33kDa in mouse kidney cell lysates. Overnight incubation with primary antibody is recommended.
Copyright © 2019 Bio-Rad Antibodies (formerly AbD Serotec)

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Rabbit anti Goat IgG (Fc):FITC STAR122F F 1 mg loader
Rabbit anti Goat IgG (Fc):HRP STAR122P C E WB 1 mg loader

Product Specific References

Further Reading

  1. Senechal, K. et al. (1996) The CRKL adaptor protein transforms fibroblasts and functions in transformation by the BCR-ABL oncogene.
    J Biol Chem. 271 (38): 23255-61.
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