CRKL antibody

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Anti CRKL Antibody gallery image 1 — Western blotting analysis of K562 lysate (35μg protein in RIPA buffer) probed with Goat anti Human CRKL antibody (**AHP693**) at 0.01μgml^-1. Primary incubation was 1 hour. Signal was detected by chemiluminescence.

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Goat anti CRKL

Product Type: Polyclonal Antibody
Isotype: Polyclonal IgG
Specificity: CRKL

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Applications
Storage Information
Batch/Lot Specific Datasheets

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Product Code Applications Pack Size List Price Your Price Qty

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AHP693 Datasheet Datasheet SDS SDS	WB	0.1 mg		Log in	Get Quote
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Goat anti Human CRKL antibody recognizes CRKL, a ~33 kDa adaptor protein shown to activate the RAS and JUN kinase signalling pathways and transform fibroblasts in a RAS-dependant manner.

CRKL is a substitute of BCR-ABL tyrosine kinase.

Target Species: Human
Species Cross-Reactivity: Target Species Cross Reactivity

Mouse; N.B. Antibody reactivity and working conditions may vary between species.
Product Form: Purified IgG - liquid
Antiserum Preparation: Antisera to CRKL were raised by repeated immunisations of goats with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
Buffer Solution: TRIS buffered saline
Preservative Stabilisers: <0.1% Sodium Azide (NaN₃)
0.5% Bovine Serum Albumin
Immunogen: Synthetic peptide sequence KIFDPQNPDENE derived from C-terminus of CRKL.
Approx. Protein Concentrations: IgG concentration 0.5 mg/ml
Regulatory: For research purposes only
Guarantee: 12 months from date of despatch

Target Species	Cross Reactivity
Mouse

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Verified	Min Dilution	Max Dilution
Western Blotting		1/200	1/500

Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.

Western Blotting: AHP693 detects a band of approximately 33kDa in mouse kidney cell lysates. Overnight incubation with primary antibody is recommended.

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