CRKL antibody
Goat anti Human CRKL antibody recognizes CRKL, a ~33 kDa adaptor protein shown to activate the RAS and JUN kinase signalling pathways and transform fibroblasts in a RAS-dependant manner.
CRKL is a substitute of BCR-ABL tyrosine kinase.
CRKL is a substitute of BCR-ABL tyrosine kinase.
Product Details
- Target Species
- Human
- Species Cross-Reactivity
-
Target Species Cross Reactivity Mouse - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Antiserum Preparation
- Antisera to CRKL were raised by repeated immunisations of goats with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
- Buffer Solution
- TRIS buffered saline
- Preservative Stabilisers
0.02% Sodium Azide 0.5% Bovine Serum Albumin - Immunogen
- Synthetic peptide sequence KIFDPQNPDENE derived from C-terminus of CRKL.
- Approx. Protein Concentrations
- IgG concentration 0.5 mg/ml
Storage Information
- Storage
- This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. - Guarantee
- 12 months from date of despatch
More Information
- UniProt
- P46109
- Entrez Gene
- CRKL
- GO Terms
- GO:0005829 cytosol
- GO:0004713 protein tyrosine kinase activity
- GO:0004871 signal transducer activity
- GO:0005070 SH3/SH2 adaptor activity
- GO:0007254 JNK cascade
- GO:0007265 Ras protein signal transduction
- Regulatory
- For research purposes only
Applications of CRKL antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Western Blotting | 1/200 | 1/500 |
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
- Western Blotting
- AHP693 detects a band of approximately 33kDa in mouse kidney cell lysates. Overnight incubation with primary antibody is recommended.
Copyright © 2021 Bio-Rad Antibodies (formerly AbD Serotec)
Secondary Antibodies Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| Rabbit anti Goat IgG (Fc):FITC | STAR122F | F | 1 mg |
|
|
| Rabbit anti Goat IgG (Fc):HRP | STAR122P | C E WB | 1 mg |
|
Product Specific References
Further Reading
-
Senechal, K. et al. (1996) The CRKL adaptor protein transforms fibroblasts and functions in transformation by the BCR-ABL oncogene.
J Biol Chem. 271 (38): 23255-61.
Fluorescent Spectraviewer
Watch the Tool Tutorial Video ▸How to Use the Spectraviewer
Watch the Tool Tutorial Video ▸- Start by selecting the application you are interested in, with the option to select an instrument from the drop down menu or create a customized instrument
- Select the fluorophores or fluorescent proteins you want to include in your panel to check compatibility
- Select the lasers and filters you wish to include
- Select combined or multi-laser view to visualize the spectra
