CREB1 antibody

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Anti CREB1 Antibody gallery image 1 — Western blot analysis of HEK 293T, HeLa, Jurkat and L02 cell lysates using Rabbit anti CREB1 antibody at a 1/3000 dilution. 3% non-fat dry milk in TBST was used for blocking

Anti CREB1 Antibody gallery image 2 — Western blot analysis of mouse and rat brain tissue lysates using Rabbit anti CREB1 antibody at a 1/3000 dilution. 3% non-fat dry milk in TBST was used for blocking

Rabbit anti CREB1

Product Type: Polyclonal Antibody
Isotype: Polyclonal IgG

Product Code	Applications	Pack Size	Your Price	List Price	Quantity
AHP2738 Datasheet	WB SDS	50 µl	Log in		Get Quote

Rabbit anti CREB1 antibody recognizes cyclin AMP-responsive element-binding protein 1, also known as CREB-1. It belongs to the family of transcription factors mediating gene expression in neuronal and non-neuronal cells (Lonze et al. 2002). CREB1 is activated in response to growth factor stimulation, electrical stimulation and synaptic activity (Shaywitz and Greenberg 1999). In mice, disruption of CREB1 function results in neurodegeneration (Mantamadiotis et al. 2002). CREB1 is overexpressed in gliomas and promotes gliomagenesis acting as a modulator of oncogenic microRNA-23a (Tan et al. 2012). CREB1, together with SETD2, contribute to cisplatin cytotoxicity regulating the ERK signalling pathway (Kim et al. 2018).

Product Details

Target Species: Human
Product Form: Purified IgG - liquid
Antiserum Preparation: Antiserum to CREB1 was raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
Buffer Solution: Phosphate buffered saline
Preservative Stabilisers: 0.02% Sodium Azide
50% Glycerol
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-341 of human CREB1

Storage Information

Storage: Store at -20^oC
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Guarantee: 12 months from date of despatch

More Information

UniProt: P16220
Entrez Gene: CREB1
GO Terms: GO:0002755 MyD88-dependent toll-like receptor signaling pathway; GO:0002756 MyD88-independent toll-like receptor signaling pathway; GO:0003712 transcription cofactor activity; GO:0006468 protein phosphorylation; GO:0007202 activation of phospholipase C activity; GO:0007268 synaptic transmission; GO:0007411 axon guidance; GO:0008063 Toll signaling pathway; GO:0010033 response to organic substance; GO:0048015 phosphatidylinositol-mediated signaling; GO:0034130 toll-like receptor 1 signaling pathway; GO:0034134 toll-like receptor 2 signaling pathway; GO:0034138 toll-like receptor 3 signaling pathway; GO:0034142 toll-like receptor 4 signaling pathway; GO:0044419 interspecies interaction between organisms; GO:0045087 innate immune response; GO:0046983 protein dimerization activity; GO:0048011 nerve growth factor receptor signaling pathway; GO:0051403 stress-activated MAPK cascade
Regulatory: For research purposes only

Applications of CREB1 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Verified	Min Dilution	Max Dilution
Western Blotting		1/500	1/3000

Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

Western Blotting: Rabbit anti CREB1 antibody detects a band of approximately 40 kDa in various cell ant tissue lysates under reducing conditions

Secondary Antibodies Available

Description	Product Code	Applications	Pack Size	Your Price	Quantity
Goat anti Rabbit IgG (Fc):Biotin	STAR121B	E WB	1 mg	Log in	Get Quote
Goat anti Rabbit IgG (Fc):FITC	STAR121F	F	1 mg	Log in	Get Quote
Goat anti Rabbit IgG (Fc):HRP	STAR121P	E WB	1 mg	Log in	Get Quote
Goat anti Rabbit IgG (H/L):HRP	STAR124P	C E WB	1 mg	Log in	Get Quote
Sheep anti Rabbit IgG:FITC	STAR34B	C F	1 mg	Log in	Get Quote
Sheep anti Rabbit IgG:RPE	STAR35A	F	1 ml	Log in	Get Quote

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