CREB Binding Protein antibody

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Rabbit anti CREB Binding Protein

Product Type
PrecisionAb Polyclonal
Isotype
Polyclonal IgG
Format
Purified
Product Code Applications Pack Size List Price Quantity
VPA00303
Datasheet
WB
MSDS
100 µl loader

Rabbit anti Human CREB binding protein antibody recognizes CREB binding protein, also known as CREBBP and CBP.

CREB binding protein is ubiquitously expressed and is involved in the transcriptional co-activation of many different transcription factors. First isolated as a nuclear protein that binds to cAMP-response element binding protein (CREB), CREB binding protein is now known to play critical roles in embryonic development, growth control, and homeostasis by coupling chromatin remodeling to transcription factor recognition. The protein encoded by the CREB binding protein gene has intrinsic histone acetyltransferase activity and also acts as a scaffold to stabilize additional protein interactions with the transcription complex. This protein acetylates both histone and non-histone proteins. This protein shares regions of very high sequence similarity with protein p300 in its bromodomain, cysteine-histidine-rich regions, and histone acetyltransferase domain. Mutations in the CREB binding protein gene cause Rubinstein-Taybi syndrome (RTS). Chromosomal translocations involving the CREB binding protein gene have been associated with acute myeloid leukemia. Alternative splicing results in multiple transcript variants encoding different isoforms (provided by RefSeq, Feb 2009).

Rabbit anti Human CREB binding protein antibody detects a band of 300 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.

Product Details

Target Species
Human
Western Blotting
Anti CREB binding protein detects a band of approximately 300 kDa in HEK293 cell lysate
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse
Rat
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Preparation
Rabbit polyclonal antibody purified by affinity chromatography
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
2% Sucrose
Immunogen
Synthetic peptide corresponding to the N-terminal region of human CREB binding protein

Storage Information

Storage
Store undiluted at -20oC, avoiding repeated freeze thaw cycles
Guarantee
As supplied, 12 months from date of despatch

More Information

UniProt
Q92793 Related reagents
Entrez Gene
CREBBP Related reagents
GO Terms
GO:0001666 response to hypoxia
GO:0002039 p53 binding
GO:0003700 sequence-specific DNA binding transcription factor activity
GO:0003713 transcription coactivator activity
GO:0004402 histone acetyltransferase activity
GO:0004871 signal transducer activity
GO:0005737 cytoplasm
GO:0006461 protein complex assembly
GO:0008270 zinc ion binding
GO:0016573 histone acetylation
GO:0016604 nuclear body
GO:0018076 N-terminal peptidyl-lysine acetylation
GO:0042592 homeostatic process
GO:0044255 cellular lipid metabolic process
GO:0044419 interspecies interaction between organisms
GO:0051577 MyoD binding
Acknowledgements
PrecisionAb is a trademark of Bio-Rad Laboratories.
Regulatory
For research purposes only

Applications of CREB Binding Protein antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000

The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Goat anti Rabbit IgG (H/L):HRP STAR208P WB 2 ml

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