CREB Binding Protein antibody
Rabbit anti CREB Binding Protein
Rabbit anti human CREB binding protein antibody recognizes the CREB-binding protein, also known as CREBBP or CBP. This antibody has been extensively validated for western blotting using whole cell lysates.
CREB Binding Protein Antibody with Control Lysate
Rabbit anti human CREB binding protein antibody recognizes the CREB-binding protein, also known as CREBBP or CBP. This antibody has been extensively validated for western blotting using whole cell lysates.
- Product Type
- PrecisionAb™ Polyclonal
- Isotype
- Polyclonal IgG
- Format
- Purified
| Product Code | Applications | Datasheet | MSDS | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|---|
| VPA00303 | WB | 100 µl | ||||
| VPA00303KT | WB | 2 Westerns | ||||
| With Lysate | 400µg |
Rabbit anti Human CREB binding protein antibody recognizes CREB binding protein, also known as CREBBP and CBP.
CREB binding protein is ubiquitously expressed and is involved in the transcriptional co-activation of many different transcription factors. First isolated as a nuclear protein that binds to cAMP-response element binding protein (CREB), CREB binding protein is now known to play critical roles in embryonic development, growth control, and homeostasis by coupling chromatin remodeling to transcription factor recognition. The protein encoded by the CREB binding protein gene has intrinsic histone acetyltransferase activity and also acts as a scaffold to stabilize additional protein interactions with the transcription complex. This protein acetylates both histone and non-histone proteins. This protein shares regions of very high sequence similarity with protein p300 in its bromodomain, cysteine-histidine-rich regions, and histone acetyltransferase domain. Mutations in the CREB binding protein gene cause Rubinstein-Taybi syndrome (RTS). Chromosomal translocations involving the CREB binding protein gene have been associated with acute myeloid leukemia. Alternative splicing results in multiple transcript variants encoding different isoforms (provided by RefSeq, Feb 2009).
Rabbit anti Human CREB binding protein antibody detects a band of 300 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.
CREB binding protein is ubiquitously expressed and is involved in the transcriptional co-activation of many different transcription factors. First isolated as a nuclear protein that binds to cAMP-response element binding protein (CREB), CREB binding protein is now known to play critical roles in embryonic development, growth control, and homeostasis by coupling chromatin remodeling to transcription factor recognition. The protein encoded by the CREB binding protein gene has intrinsic histone acetyltransferase activity and also acts as a scaffold to stabilize additional protein interactions with the transcription complex. This protein acetylates both histone and non-histone proteins. This protein shares regions of very high sequence similarity with protein p300 in its bromodomain, cysteine-histidine-rich regions, and histone acetyltransferase domain. Mutations in the CREB binding protein gene cause Rubinstein-Taybi syndrome (RTS). Chromosomal translocations involving the CREB binding protein gene have been associated with acute myeloid leukemia. Alternative splicing results in multiple transcript variants encoding different isoforms (provided by RefSeq, Feb 2009).
Rabbit anti Human CREB binding protein antibody detects a band of 300 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.
Product Details
- Target Species
- Human
- Western Blotting
- Anti CREB binding protein detects a band of approximately 300 kDa in HEK293 cell lysate
- Species Cross-Reactivity
-
Target Species Cross Reactivity Mouse Rat - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Product Form
- Purified IgG - liquid
- Preparation
- Rabbit polyclonal antibody purified by affinity chromatography
- Preparation
- 20μl Rabbit polyclonal antibody purified by affinity chromatography
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
2% Sucrose - Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
2% Sucrose - Immunogen
- Synthetic peptide corresponding to the N-terminal region of human CREB binding protein
- Instructions For Use
- Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.
Positive Control Lysate
- Lysate Composition
- 400μg HEK293 lysate lyophilized in RIPA buffer
- Lysate Reconstitution
- - If using DDT reconstitute the lyophilized lysate with 190μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 10μl 2M DTT.
- If using BME reconstitute the lyophilized lysate with 180μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 20μl BME.
Heat at 95°C for 5 minutes. For 10 well mini gels load 25μl. For other gel and comb formats please refer to the PrecisionAb western blotting protocol.
Storage Information
- Storage
- Store undiluted at -20oC, avoiding repeated freeze thaw cycles
- Storage
- Antibody: Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage. - Shelf Life
- As supplied, 12 months from date of despatch
- Shelf Life
- As supplied, 12 months from date of despatch
More Information
- UniProt
- Q92793 Related reagents
- Entrez Gene
- CREBBP Related reagents
- GO Terms
- GO:0001666 response to hypoxia
- GO:0002039 p53 binding
- GO:0003700 sequence-specific DNA binding transcription factor activity
- GO:0003713 transcription coactivator activity
- GO:0004402 histone acetyltransferase activity
- GO:0004871 signal transducer activity
- GO:0005737 cytoplasm
- GO:0006461 protein complex assembly
- GO:0008270 zinc ion binding
- GO:0016573 histone acetylation
- GO:0016604 nuclear body
- GO:0018076 N-terminal peptidyl-lysine acetylation
- GO:0042592 homeostatic process
- GO:0044255 cellular lipid metabolic process
- GO:0044419 interspecies interaction between organisms
- GO:0051577 MyoD binding
- Acknowledgements
- PrecisionAb™ is a trademark of Bio-Rad Laboratories
- Acknowledgements
- PrecisionAb™ is a trademark of Bio-Rad Laboratories
- Regulatory
- For research purposes only
Applications of CREB Binding Protein antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Western Blotting | 1/1000 | ||
| Western Blotting | 1/1000 |
PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
Copyright © 2018 Bio-Rad Antibodies (formerly AbD Serotec)
Secondary Antibodies Available
| Description | Product Code | Pack Size | Applications | List Price | Quantity |
|---|---|---|---|---|---|
| Goat anti Rabbit IgG (H/L):HRP | STAR208P | 2 ml | WB | ||
| Goat anti Rabbit IgG (H/L):HRP | STAR208P | 2 ml | WB |
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