Creatine Phosphokinase antibody

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Goat anti Human Creatine Phosphokinase (MM)

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
2400-1804G E WB 0.5 ml
Goat anti Human creatine phosphokinase MM antibody recognizes creatine phosphokinase, also known as creatine kinase (CK), is an enzyme expressed by various tissues and cell types. CK catalyses the conversion of creatine and consumes adenosine triphosphate (ATP) to create phosphocreatine and adenosine diphosphate (ADP).

In cells, the cytosolic CK enzymes consist of two subunits, which can be either B (brain type) or M (muscle type). There are three different isoenzymes: CKMM, CKBB and CKMB.

Goat anti Human Creatine Phosphokinase (MM) antibody recognizes the CKMM isoenzyme and was found to react ~50% with CKMB when tested in ELISA.

Product Details

Target Species
Human
Product Form
Purified IgG - liquid
Antiserum Preparation
Antisera to human creatine phosphokinase were raised by repeated immunisations of goats with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography on protein G.
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09%Sodium Azide (NaN3)
Immunogen
CKMM from skeletal muscle
Approx. Protein Concentrations
IgG concentration 5.0 mg/ml

Storage Information

Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.

More Information

UniProt
P06732 Related reagents
Entrez Gene
CKM Related reagents
GO Terms
GO:0004111 creatine kinase activity
GO:0005829 cytosol
GO:0005524 ATP binding
GO:0006600 creatine metabolic process
Regulatory
For research purposes only

Applications of Creatine Phosphokinase antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA
Western Blotting 1/500 1/1000
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using the appropriate negative/positive controls.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Rabbit anti Goat IgG (Fc):FITC STAR122F 1 mg F
Rabbit anti Goat IgG (Fc):HRP STAR122P 1 mg C E WB

Product Specific References

References for Creatine Phosphokinase antibody

  1. Ponticos, M. et al. (1998) Dual regulation of the AMP-activated protein kinase provides a novel mechanism for the control of creatine kinase in skeletal muscle.
    EMBO J. 17 (6): 1688-99.