CPSF3 antibody

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Rabbit anti CPSF3

Product Type
PrecisionAb Polyclonal
Isotype
Polyclonal IgG
Format
Purified
Product Code Applications Pack Size List Price Quantity
VPA00581
Datasheet
WB
SDS
100 µl loader

Rabbit anti Human CPSF3 antibody recognizes CPSF3 also known as cleavage and polyadenylation specificity factor subunit 3, also known as mRNA 3′-end-processing endonuclease CPSF-73 or cleavage and polyadenylation specificity factor 73 kDa subunit.

CPSF3 gene encodes a member of the metallo-beta-lactamase family. The encoded protein is a 73 kDa subunit of the cleavage and polyadenylation specificity factor and functions as an endonuclease that recognizes the pre-mRNA 3′-cleavage site AAUAAA prior to polyadenylation. It also cleaves after the pre-mRNA sequence ACCCA during histone 3′-end pre-mRNA processing (provided by RefSeq, Oct 2012).

Rabbit anti Human CPSF3 antibody detects a band of 77 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.

Product Details

Target Species
Human
Western Blotting
Anti CPSF3 detects a band of approximately 77 kDa in HeLa cell lysates.
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid.
Preparation
Rabbit polyclonal antibody purified by affinity chromatography.
Buffer Solution
Phosphate buffered saline.
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
2% Sucrose.
Immunogen
Synthetic peptide directed towards the C terminal region of human CPSF3

Storage Information

Storage
Store undiluted at -20°C, avoiding repeated freeze thaw cycles.
Guarantee
12 months from date of despatch

More Information

UniProt
Q9UKF6
Entrez Gene
CPSF3
GO Terms
GO:0005515 protein binding
GO:0000398 nuclear mRNA splicing, via spliceosome
GO:0003723 RNA binding
GO:0030529 ribonucleoprotein complex
GO:0004521 endoribonuclease activity
GO:0046872 metal ion binding
GO:0005847 mRNA cleavage and polyadenylation specificity factor complex
GO:0006369 termination of RNA polymerase II transcription
GO:0006378 mRNA polyadenylation
GO:0006379 mRNA cleavage
GO:0006398 histone mRNA 3'-end processing
GO:0006406 mRNA export from nucleus
GO:0008409 5'-3' exonuclease activity
Acknowledgements
PrecisionAb is a trademark of Bio-Rad Laboratories
Regulatory
For research purposes only.

Applications of CPSF3 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000

The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Goat anti Rabbit IgG (H/L):HRP STAR208P WB 2 ml