COX4I1 antibody

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Anti COX4I1 Antibody (PrecisionAb Polyclonal Antibody) thumbnail image 1

Anti COX4I1 Antibody (PrecisionAb Polyclonal Antibody) thumbnail image 2

Anti COX4I1 Antibody (PrecisionAb Polyclonal Antibody) thumbnail image 3

Anti COX4I1 Antibody (PrecisionAb Polyclonal Antibody) gallery image 1 — Western blot analysis of whole cell lysates probed with COX4I1 antibody (**VPA00544**) followed by detection with HRP conjugated Goat anti Rabbit IgG antibody (1/10,000, STAR208P) and visualized on the ChemiDoc MP with 7 second exposure. Arrow points to COX4I1 (molecular weight 17 kDa).

Anti COX4I1 Antibody (PrecisionAb Polyclonal Antibody) gallery image 2 — Western blot analysis of COX4I1 CRISPR knockout 293T (COX4I1 KO) and wild type 293T (WT) whole cell lysates probed with Rabbit anti COX4I1 antibody **(VPA00544**) followed by detection with HRP conjugated Goat anti Rabbit IgG (1/10,000 STA208P) and hFAB rhodamine anti-tubulin primary antibody (12004165) and visualized on the ChemiDoc MP with an 167 second (chemiluminescent channel) and 30 second (rhodamine channel) exposure.

Anti COX4I1 Antibody (PrecisionAb Polyclonal Antibody) gallery image 3 — Normalized signal intensity of COX4I1 from CRISPR knockout and wild type lysates. Signal intensity was normalized to the tubulin signal.

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Rabbit anti COX4I1

Product Type: PrecisionAb Polyclonal
Isotype: Polyclonal IgG
Format: Purified
Specificity: COX4I1

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Product Code Applications Pack Size List Price Your Price Qty

List Price	Your Price
VPA00544 Datasheet Datasheet SDS SDS	WB	100 µl		Log in	Get Quote
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Rabbit anti Human COX4I1 antibody recognizes COX4l1 also known as cytochrome c oxidase subunit 4 isoform 1, mitochondrial, COX IV-1, cytochrome c oxidase polypeptide IV or cytochrome c oxidase subunit 4 isoform 1.

Cytochrome c oxidase (COX) is the terminal enzyme of the mitochondrial respiratory chain. It is a multi-subunit enzyme complex that couples the transfer of electrons from cytochrome c to molecular oxygen and contributes to a proton electrochemical gradient across the inner mitochondrial membrane. The complex consists of 13 mitochondrial- and nuclear-encoded subunits. The mitochondrially-encoded subunits perform the electron transfer and proton pumping activities. The functions of the nuclear-encoded subunits are unknown but they may play a role in the regulation and assembly of the complex. COX4I1 encodes the nuclear-encoded subunit IV isoform 1 of the human mitochondrial respiratory chain enzyme. It is located at the 3' of the NOC4 (neighbor of COX4) gene in a head-to-head orientation, and shares a promoter with it (provided by RefSeq, Jul 2008).

Rabbit anti Human COX4l1 antibody detects a band of 17 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.

Target Species: Human
Western Blotting: Anti COX4l1 detects a band of approximately 17 kDa in Jurkat cell lysates.
Species Cross-Reactivity: Target Species Cross Reactivity

Mouse; N.B. Antibody reactivity and working conditions may vary between species.
Product Form: Purified IgG - liquid.
Preparation: Rabbit polyclonal antibody purified by affinity chromatography.
Buffer Solution: Phosphate buffered saline.
Preservative Stabilisers: 0.09% Sodium Azide (NaN₃).
Immunogen: KLH conjugated synthetic peptide corresponding to amino acid 25-54 of human COX4I1
Approx. Protein Concentrations: IgG concentration 0.5 mg/ml
Regulatory: For research purposes only.
Guarantee: 12 months from date of despatch.
Acknowledgements: PrecisionAb is a trademark of Bio-Rad Laboratories.

Target Species	Cross Reactivity
Mouse

Store undiluted at -20°C, avoiding repeated freeze thaw cycles.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Verified	Min Dilution	Max Dilution
Western Blotting		1/1000

The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.

Description	Product Code	Applications	Pack Size	Your Price
Goat anti Rabbit IgG (H/L):HRP	STAR208P	WB	2 ml	Log in	Get Quote
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Description	Goat anti Rabbit IgG (H/L):HRP

UniProt: P13073
Entrez Gene: COX4I1
GO Terms: GO:0005515 protein binding; GO:0004129 cytochrome-c oxidase activity; GO:0005634 nucleus; GO:0005743 mitochondrial inner membrane; GO:0022904 respiratory electron transport chain