Cortisol antibody

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Donkey anti Cortisol

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
2330-5000 E R 1 ml
Donkey anti Cortisol antibody recognizes the glucocorticoid hormone cortisol, produced in the adrenal cortex and involved in responses to stress.

The following cross reactivities have been observed:

Cortisol100%
Prednisolone38%
11-Deoxycortisol2.5%
17a-OHP0.4%
Corticosterone<0.1%
Cortisone23%

Product Details

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Broad Expected from Sequence
Fish Expected from Sequence
Rainbow Trout
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein G.
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Immunogen
Cortisol-3-(O-carboxymethyl) oxime-BSA
Approx. Protein Concentrations
5.0 mg/ml

Storage Information

Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.

More Information

Regulatory
For research purposes only

Applications of Cortisol antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 1/100
Radioimmunoassays 1/100
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using the appropriate negative/positive controls.

Product Specific References

References for Cortisol antibody

  1. Johansen, I.B. et al. (2011) Cortisol response to stress is associated with myocardial remodeling in salmonid fishes.
    J Exp Biol. 214: 1313-21.
  2. Vindas, M.A. et al. (2014) Frustrative reward omission increases aggressive behaviour of inferior fighters.
    Proc Biol Sci. 281: 20140300.
  3. Sørensen, C. et al. (2011) Cortisol reduces cell proliferation in the telencephalon of rainbow trout (Oncorhynchus mykiss).
    Physiol Behav. 102: 518-23.
  4. Sørensen, C. et al. (2012) Social stress reduces forebrain cell proliferation in rainbow trout (Oncorhynchus mykiss).
    Behav Brain Res. 227 (2): 311-8.