Cortactin antibody

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Rabbit anti Cortactin

Product Type
PrecisionAb™ Polyclonal
Isotype
Polyclonal IgG
Format
Purified
Product Code Applications Pack Size List Price Quantity
VPA00430
Datasheet
WB
MSDS
100 µl loader

Rabbit anti Human cortactin antibody recognizes the Src substrate cortactin, also known as amplaxin, ems1 sequence (mammary tumor and squamous cell carcinoma-associated (p80/85 src substrate) or oncogene EMS1.

CTTN is overexpressed in breast cancer and squamous cell carcinomas of the head and neck (Patel et al. 1996). Cortactin is localized in the cytoplasm and in areas of the cell-substratum contacts. CTTN has two roles: (1) regulating the interactions between components of adherens-type junctions and (2) organizing the cytoskeleton and cell adhesion structures of epithelia and carcinoma cells. During apoptosis, the encoded protein is degraded in a caspase dependent manner. The aberrant regulation of CTTN contributes to tumor cell invasion and metastasis (Li et al. 2001). Three splice variants that encode different isoforms have been identified for CTTN (provided by RefSeq, May 2010).

Rabbit anti Human cortactin antibody detects a band of 80 kDa and has been extensively validated for western blotting using whole cell lysates.

Product Details

Target Species
Human
Western Blotting
Anti cortactin detects a band of approximately 80 kDa in A431 cell lysates.
Species Cross-Reactivity
Target SpeciesCross Reactivity
Rat
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid.
Preparation
Rabbit polyclonal antibody purified by affinity chromatography.
Buffer Solution
Phosphate buffered saline.
Preservative Stabilisers
0.09% Sodium Azide (NaN3).
Immunogen
KLH conjugated synthetic peptide corresponding to amino acid 273-302 of human cortactin

Storage Information

Storage
Store undiluted at -20°C, avoiding repeated freeze thaw cycles.
Guarantee
As supplied, 12 months from date of despatch.

More Information

UniProt
Q14247 Related reagents
Entrez Gene
CTTN Related reagents
GO Terms
GO:0005515 protein binding
GO:0001726 ruffle
GO:0005625 soluble fraction
GO:0005856 cytoskeleton
GO:0005938 cell cortex
GO:0030027 lamellipodium
Acknowledgements
PrecisionAb is a trademark of Bio-Rad Laboratories
Regulatory
For research purposes only.

Applications of Cortactin antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000

The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Goat anti Rabbit IgG (H/L):HRP STAR208P WB 2 ml

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