Complement Factor H antibody

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Anti Complement Factor H Antibody (PrecisionAb Polyclonal Antibody) gallery image 1 — Western blot analysis of human plasma samples probed with complement factor H antibody followed by detection with HRP conjugated Donkey anti Sheep/Goat IgG (1/10,000, STAR88P) and visualized on the ChemiDoc MP with 60 second exposure. Arrow points to complement factor H (molecular weight 155 kDa)

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Goat anti Complement Factor H

Product Type: PrecisionAb Polyclonal
Isotype: Polyclonal IgG
Format: Purified
Specificity: Complement Factor H

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Goat anti Human complement factor H antibody recognizes complement factor H, also known as H factor 1.

CFH, the gene encoding complement factor H, is a member of the regulator of complement activation (RCA) gene cluster. Complement factor H contains twenty short consensus repeat (SCR) domains, is secreted into the bloodstream and has an essential role in the regulation of complement activation, restricting this innate defense mechanism to microbial infections. Mutations in complement factor H have been associated with hemolytic-uremic syndrome (HUS) and chronic hypocomplementemic nephropathy. Alternate transcriptional splice variants, encoding different isoforms, have been characterized (provided by RefSeq, Oct 2011).

Goat anti Human complement factor H antibody detects a band of 155 kDa. The antibody has been extensively validated for western blotting using human plasma samples.

Target Species: Human
Western Blotting: Anti complement factor H detects a band of approximately 155 kDa in human plasma samples.
Product Form: Purified IgG - liquid
Preparation: Goat polyclonal antibody purified by affinity chromatography.
Buffer Solution: TRIS buffered saline.
Preservative Stabilisers: 0.02% Sodium Azide (NaN₃) 0.5% BSA
Immunogen: Peptide with the sequence C-HLVPDRKKDQYK, from the internal region of the protein sequence
Regulatory: For research purposes only.
Guarantee: 12 months from date of despatch.
Acknowledgements: PrecisionAb is a trademark of Bio-Rad Laboratories.

Store undiluted at -20°C, avoiding repeated freeze thaw cycles.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Verified	Min Dilution	Max Dilution
Western Blotting		1/1000

The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.

UniProt: P08603
Entrez Gene: CFH