Complement Factor H antibody

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Sheep anti Human Complement Factor H

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
4400-9504 E ID 1 ml
Sheep anti Human complement Factor H antibody recognizes human Factor H, a cofactor involved in the regulation of the alternative complement pathway.In IEP a single arc is observed against human plasma. Identity has been confirmed by double diffusion against human plasma and a known anti-human Factor H antibody. Deficiency in factor H can lead to diseases such as Hemolytic-uremic syndrome and chronic hypocomplementemic nephropathy.

Sheep anti Human Complement Factor H antibody has been used successfully as a capture reagent for the measurement of murine factor H in mouse serum samples by sandwich ELISA in conjunction with Mouse anti Human Factor H antibody, clone OX-23 (MCA508G ) as a detection reagent (Daniels-Treffandier et al. 2016).

Product Details

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Goat
Guinea Pig
Bovine
Dog
Chicken
Horse
Rat
Rabbit
Mouse
Cat
Pig
Bovine
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Ig fraction - liquid
Preparation
Ig fraction prepared by ammonium sulphate precipitation and ion exchange chromatography.
Buffer Solution
Glycine buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
0.1% EACA
0.01% Benzamidine
1mM EDTA
Immunogen
Human Factor H, purified from plasma.

Storage Information

Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
Please see label for expiry date.

More Information

UniProt
P08603 Related reagents
Entrez Gene
CFH Related reagents
Regulatory
For research purposes only

Applications of Complement Factor H antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA
Immunodiffusion
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using the appropriate negative/positive controls.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Rabbit anti Sheep IgG (H/L):Biotin 5184-2304 1.5 ml C E F WB
Donkey anti Sheep/Goat IgG:DyLight®488 STAR88D488GA 0.1 mg F IF
Donkey anti Sheep/Goat IgG:DyLight®549 STAR88D549GA 0.1 mg F IF
Donkey anti Sheep/Goat IgG:DyLight®649 STAR88D649GA 0.1 mg F IF
Donkey anti Sheep/Goat IgG:FITC STAR88F 1 mg C
Donkey anti Sheep/Goat IgG:HRP STAR88P 1 ml C E P WB

Product Specific References

References for Complement Factor H antibody

  1. Daniels-Treffandier, H. et al. (2016) Impact of Reducing Complement Inhibitor Binding on the Immunogenicity of Native Neisseria meningitidis Outer Membrane Vesicles.
    PLoS One. 11 (2): e0148840.