Collagen V antibody

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Rabbit anti Human Collagen V

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
2150-0180 C E IF P* 0.5 ml
Rabbit anti Human collagen V atibody recognizes human collagen V and demonstrates th following cross reactivities as demonstrated by ELISA:
Human collagen type V 100%
Human collagen type IV <5%
Human collagen type I & III <3%
Human plasma proteins <0.5%

Product Details

Target Species
Human
Product Form
Purified IgG - lyophilised
Reconstitution
Reconstitute with 0.5 ml distilled water containing 0.1% bovine serum albumin
Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution. For long term storage the addition of 0.09% sodium azide is recommended.
Preparation
Purified IgG prepared by affinity chromatography
Buffer Solution
Phosphate/borate buffered saline.
Preservative Stabilisers
1% Dextran
1% Mannitol
Immunogen
Native human collagen V from placenta.
Approx. Protein Concentrations
IgG concentration 0.1 mg/ml after reconstitution

Storage Information

Storage
Prior to reconstitution store at +4oC.
After reconstitution store at -20oC.
Storage in frost-free freezers is not recommended. This product should be stored undiluted.
Avoid repeated freezing and thawing as this may denature the antibody.
Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
12 months from date of reconstitution.

More Information

UniProt
P20908 Related reagents
P05997 Related reagents
P25940 Related reagents
Entrez Gene
COL5A2 Related reagents
COL5A1 Related reagents
COL5A3 Related reagents
GO Terms
GO:0007155 cell adhesion
GO:0005178 integrin binding
GO:0005588 collagen type V
GO:0007411 axon guidance
GO:0008201 heparin binding
GO:0030199 collagen fibril organization
GO:0032964 collagen biosynthetic process
GO:0035313 wound healing, spreading of epidermal cells
GO:0043206 fibril organization
GO:0043394 proteoglycan binding
GO:0043588 skin development
GO:0045112 integrin biosynthetic process
GO:0048407 platelet-derived growth factor binding
GO:0048592 eye morphogenesis
GO:0005201 extracellular matrix structural constituent
GO:0005518 collagen binding
Regulatory
For research purposes only

Applications of Collagen V antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 1/1500
Immunofluorescence
Immunohistology - Frozen 1/20
Immunohistology - Paraffin 1 1/20
  1. 1This product requires protein digestion pre-treatment of paraffin sections. 1 mg/ml pepsin in 0.5M acetic acid for 2 hours at 37ÂșC and multiple buffer washes is recommended.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using the appropriate negative/positive controls.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Sheep anti Rabbit IgG:Biotin 2AB02B 1 ml C E P WB
Goat anti Rabbit IgG (Fc):Biotin STAR121B 1 mg E WB
Goat anti Rabbit IgG (Fc):FITC STAR121F 1 mg F
Goat anti Rabbit IgG (Fc):HRP STAR121P 1 mg E WB
Goat anti Rabbit IgG (H/L):HRP STAR124P 1 mg C E WB
Sheep anti Rabbit IgG:FITC STAR34B 1 mg C F
Sheep anti Rabbit IgG:RPE STAR35A 1 ml F
Sheep anti Rabbit IgG:DyLight®488 STAR36D488GA 0.1 mg F IF WB
Sheep anti Rabbit IgG:DyLight®549 STAR36D549GA 0.1 mg F IF
Sheep anti Rabbit IgG:DyLight®649 STAR36D649GA 0.1 mg F IF
Sheep anti Rabbit IgG:DyLight®680 STAR36D680GA 0.1 mg F IF WB
Sheep anti Rabbit IgG:Dylight®800 STAR36D800GA 0.1 mg F IF WB

Useful Reagents Available

Description Product Code Pack Size Applications List Price Quantity
Antigen Retrieval Buffer, pH8.0 BUF025A 500 ml P
Antigen Retrieval Buffer, pH8.0 BUF025C 50 ml P

Product Specific References

References for Collagen V antibody

  1. Evans, M. J. et al. (2002) Fibroblast growth factor-2 during postnatal development of the tracheal basement membrane
    Am J Physiol Lung Cell Mol Physiol 283: 1263 - 1270
  2. Mohan, H. et al. (2010) Extracellular matrix in multiple sclerosis lesions: Fibrillar collagens, biglycan and decorin are upregulated and associated with infiltrating immune cells.
    Brain Pathol. 20: 966-75.