Collagen V antibody

Rabbit anti Human Collagen V

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Specificity
Collagen V

Product Code Applications Pack Size List Price Your Price Qty
2150-0180
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C E IF P * 0.5 ml loader
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Rabbit anti Human collagen V atibody recognizes human collagen V and demonstrates th following cross reactivities as demonstrated by ELISA:
Human collagen type V 100%
Human collagen type IV <5%
Human collagen type I & III <3%
Human plasma proteins <0.5%

Target Species
Human
Product Form
Purified IgG - lyophilized
Reconstitution
Reconstitute with 0.5 ml distilled water containing 0.1% bovine serum albumin
Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution. For long term storage the addition of 0.09% sodium azide is recommended.
Preparation
Purified IgG prepared by affinity chromatography
Buffer Solution
0.15M Sodium Chloride
0.1M Sodium Phosphate
Preservative Stabilisers
1% Mannitol
1% Dextran
Immunogen
Native human collagen V from placenta.
Approx. Protein Concentrations
IgG concentration 0.1 mg/ml after reconstitution
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

Prior to reconstitution store at +4oC.
After reconstitution store at -20oC.
Storage in frost-free freezers is not recommended. This product should be stored undiluted.
Avoid repeated freezing and thawing as this may denature the antibody.
Should this product contain a precipitate we recommend microcentrifugation before use.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 1/1500
Immunofluorescence
Immunohistology - Frozen 1/20
Immunohistology - Paraffin 1 1/20
  1. 1This product requires protein digestion pre-treatment of paraffin sections. 1 mg/ml pepsin in 0.5M acetic acid for 2 hours at 37ºC and multiple buffer washes is recommended.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using the appropriate negative/positive controls.

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References for Collagen V antibody

  1. Evans, M. J. et al. (2002) Fibroblast growth factor-2 during postnatal development of the tracheal basement membrane
    Am J Physiol Lung Cell Mol Physiol 283: 1263 - 1270
  2. Mohan, H. et al. (2010) Extracellular matrix in multiple sclerosis lesions: Fibrillar collagens, biglycan and decorin are upregulated and associated with infiltrating immune cells.
    Brain Pathol. 20: 966-75.

RRID
AB_620311
UniProt
P20908
P05997
P25940
Entrez Gene
COL5A2
COL5A1
COL5A3
GO Terms
GO:0007155 cell adhesion
GO:0005178 integrin binding
GO:0005588 collagen type V
GO:0007411 axon guidance
GO:0008201 heparin binding
GO:0030199 collagen fibril organization
GO:0032964 collagen biosynthetic process
GO:0035313 wound healing, spreading of epidermal cells
GO:0043206 fibril organization
GO:0043394 proteoglycan binding
GO:0043588 skin development
GO:0045112 integrin biosynthetic process
GO:0048407 platelet-derived growth factor binding
GO:0048592 eye morphogenesis
GO:0005201 extracellular matrix structural constituent
GO:0005518 collagen binding
View more products with COLLAGEN specificity

Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

View all Anti-Human Products