Collagen II antibody
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Rabbit anti Human collagen II antibody recognizes human Collagen type II, an extracellular matrix protein found mainly in cartilaginous tissue. The antibody reacts with both native and heat denatured forms and shows minimal cross reactivity with Collagen type I, III, IV and V, as demonstrated by competitive ELISA.
Product Details
- Target Species
- Human
- Product Form
- Purified IgG - lyophilised
- Reconstitution
- Reconstitute with 0.5ml distilled water
Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution. For long term storage the addition of 0.09% sodium azide and 1% BSA is recommended. - Preparation
- Purified IgG prepared by affinity chromatography on immobilized human Collagen type II
- Buffer Solution
- Phosphate/Borate buffered saline
- Preservative Stabilisers
- 1% Dextran
1% Mannitol - Immunogen
- Human Collagen type II from cartilage.
- Approx. Protein Concentrations
- IgG concentration 0.1 mg/ml after reconstitution
Storage Information
- Storage
- Prior to reconstitution store at +4oC.
After reconstitution store at -20oC.
Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. - Guarantee
- 12 months from date of despatch
More Information
Applications of Collagen II antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA | 1/500 | 1/2000 | |
| Immunohistology - Frozen | 1/10 | 1/50 |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using the appropriate negative/positive controls.
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Secondary Antibodies Available
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| Goat anti Rabbit IgG (H/L):HRP | STAR124P | C E WB | 1 mg |
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| Sheep anti Rabbit IgG:FITC | STAR34B | C F | 1 mg |
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| Sheep anti Rabbit IgG:RPE | STAR35A | F | 1 ml |
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| Sheep anti Rabbit IgG:DyLight®488 | STAR36D488GA | F IF WB | 0.1 mg | ||
| Sheep anti Rabbit IgG:DyLight®680 | STAR36D680GA | F IF WB | 0.1 mg | ||
| Sheep anti Rabbit IgG:Dylight®800 | STAR36D800GA | F IF WB | 0.1 mg |
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