Collagen I/III antibody
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Rabbit anti Human collagen I/III antibody recognizes human collagen types I and III, showing minimum cross reactivity with the following:
This antibody has been cross adsorbed against human plasma proteins. Human plasma proteins do not interfere with the binding of Rabbit anti Human collagen I/III antibody (2150-2210) to collagen.
| Human collagen type IV | >3% |
| Human collagen type V | >2% |
This antibody has been cross adsorbed against human plasma proteins. Human plasma proteins do not interfere with the binding of Rabbit anti Human collagen I/III antibody (2150-2210) to collagen.
Product Details
- Target Species
- Human
- Product Form
- Purified IgG - lyophilised
- Reconstitution
- Reconstitute with 0.5 ml distilled water
Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution. For long term storage the addition of 0.09% sodium azide is recommended. - Antiserum Preparation
- Antiserum to human collagen type I and III was raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
- Buffer Solution
- 0.15M Sodium Chloride
0.1M Sodium Phosphate - Preservative Stabilisers
- 1% Mannitol
1% Dextran - Immunogen
- Collagen types I and III from human placenta.
- Approx. Protein Concentrations
- IgG concentration 0.1 mg/ml after reconstitution.
Storage Information
- Storage
- Prior to reconstitution store at +4oC.
After reconstitution store at -20oC.
Storage in frost-free freezers is not recommended. This product should be stored undiluted.
Avoid repeated freezing and thawing as this may denature the antibody.
Should this product contain a precipitate we recommend microcentrifugation before use. - Guarantee
- Guaranteed until date of expiry. Please see product label.
More Information
Applications of Collagen I/III antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA | 1/4000 | ||
| Immunofluorescence | 1/20 | ||
| Immunohistology - Frozen | 1/20 |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using the appropriate negative/positive controls.
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Secondary Antibodies Available
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| Goat anti Rabbit IgG (Fc):Biotin | STAR121B | E WB | 1 mg |
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| Goat anti Rabbit IgG (H/L):HRP | STAR124P | C E WB | 1 mg |
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| Sheep anti Rabbit IgG:FITC | STAR34B | C F | 1 mg |
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| Sheep anti Rabbit IgG:RPE | STAR35A | F | 1 ml |
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| Sheep anti Rabbit IgG:DyLight®488 | STAR36D488GA | F IF WB | 0.1 mg | ||
| Sheep anti Rabbit IgG:DyLight®680 | STAR36D680GA | F IF WB | 0.1 mg | ||
| Sheep anti Rabbit IgG:Dylight®800 | STAR36D800GA | F IF WB | 0.1 mg |
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