Collagen I/III antibody
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Rabbit anti Human Collagen I/III
- Product Type
- Polyclonal Antibody
- Isotype
- Polyclonal IgG
- Specificity
- Collagen I/III
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Rabbit anti Human collagen I/III antibody recognizes human collagen types I and III, showing minimum cross reactivity with the following:
This antibody has been cross adsorbed against human plasma proteins. Human plasma proteins do not interfere with the binding of Rabbit anti Human collagen I/III antibody (2150-2210) to collagen. |
- Target Species
- Human
- Product Form
- Purified IgG - lyophilized
- Reconstitution
- Reconstitute with 0.5 ml distilled water
Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution. For long term storage the addition of 0.09% sodium azide is recommended. - Antiserum Preparation
- Antiserum to human collagen type I and III was raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
- Buffer Solution
- 0.15M Sodium Chloride
0.1M Sodium Phosphate - Preservative Stabilisers
- 1% Mannitol
1% Dextran - Immunogen
- Collagen types I and III from human placenta.
- Approx. Protein Concentrations
- IgG concentration 0.1 mg/ml after reconstitution.
- Regulatory
- For research purposes only
- Guarantee
- Guaranteed until date of expiry. Please see product label.
Prior to reconstitution store at +4oC.
After reconstitution store at -20oC.
Storage in frost-free freezers is not recommended. This product should be stored undiluted.
Avoid repeated freezing and thawing as this may denature the antibody.
Should this product contain a precipitate we recommend microcentrifugation before use.
After reconstitution store at -20oC.
Storage in frost-free freezers is not recommended. This product should be stored undiluted.
Avoid repeated freezing and thawing as this may denature the antibody.
Should this product contain a precipitate we recommend microcentrifugation before use.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA |  |
1/4000 | |
| Immunofluorescence | |
1/20 | |
| Immunohistology - Frozen | |
1/20 |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using the appropriate negative/positive controls.
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