Collagen I antibody

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Anti Human Collagen I Antibody gallery image 1 — Sandwich ELISA analysis of human ferritin using Mouse anti Human collagen I/II/III antibody, clone NLI/22 (2150-2203) as a capture reagent and biotinylated Mouse anti Human collagen I antibody, (**131008**) as a detection reagent with native human collagen (2150-0030) as antigen. Detection is by HRP conjugated streptavidin and substrate. Microtitre plate is read at O.D. 450 nm on the Bio-Rad iMark Microplate Absorbance Reader (Bio-Rad Catalog 168-1135). Normal serum (green) and plasma (orange) samples are included diluted 1:4.

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Goat anti Human Collagen I:Biotin

Product Type: Polyclonal Antibody
Isotype: Polyclonal IgG
Specificity: Collagen I

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Product Code Applications Pack Size List Price Your Price Qty

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131008 Datasheet Datasheet SDS SDS	E	0.2 mg		Log in	Get Quote
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Goat anti Human collagen I antibody recognizes human type 1 collagen.

Goat anti Human collagen I antibody has been cross-adsorbed against collagen types II, III, IV, V and VI. Results from ELISA demonstrate <10% cross-reactivity with other collagen types.

Target Species: Human
Product Form: Purified IgG conjugated to Biotin - liquid
Antiserum Preparation: Antisera to human type 1 collagen were raised by repeated immunisations of goats with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
Buffer Solution: Phosphate buffered saline
Preservative Stabilisers: <0.1% Sodium Azide (NaN₃)
Immunogen: Human type 1 collagen.
Approx. Protein Concentrations: Ig concentration 0.4 mg/ml
Regulatory: For research purposes only
Guarantee: Guaranteed for 12 months from the date of despatch or until the date of expiry, whichever comes first. Please see label for expiry date.

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Verified	Min Dilution	Max Dilution
ELISA		1/200	1/2000

Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.