Collagen I antibody

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Goat anti Human Collagen I:Biotin

Goat anti Human Collagen I

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Product Code Applications Pack Size List Price Quantity
131008
Datasheet
E
SDS
0.2 mg loader

131001
Datasheet
C E
SDS
0.2 mg loader

Goat anti Human collagen I antibody recognizes human type 1 collagen.

Goat anti Human collagen I antibody has been cross-adsorbed against collagen types II, III, IV, V and VI. Results from ELISA demonstrate <10% cross-reactivity with other collagen types.

Product Details

Target Species
Human
Product Form
Purified IgG conjugated to Biotin - liquid
Product Form
Purified IgG - liquid
Antiserum Preparation
Antisera to human type 1 collagen were raised by repeated immunisations of goats with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
Buffer Solution
Phosphate buffered saline
Buffer Solution
Borate buffered saline
Preservative Stabilisers
0.1% Sodium Azide (NaN3)
Preservative Stabilisers
None present
Immunogen
Human type 1 collagen.
Approx. Protein Concentrations
Ig concentration 0.4 mg/ml
Approx. Protein Concentrations
Ig concentration 0.4 mg/ml

Storage Information

Storage
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Storage
Store at -20oC only.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Guarantee
12 months from date of despatch
Guarantee
Guaranteed until date of expiry. Please see product label.

More Information

UniProt
P02452
P08123
Entrez Gene
COL1A1
COL1A2
Regulatory
For research purposes only

Applications of Collagen I antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 1/200 1/2000
ELISA
Immunohistology - Frozen 1/10 1/20
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Rabbit anti Goat IgG (Fc):FITC STAR122F F 1 mg loader
Rabbit anti Goat IgG (Fc):HRP STAR122P C E WB 1 mg loader

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