CNTF antibody

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Rabbit anti Human CNTF

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
AHP1277 E FN P WB 0.1 mg
Rabbit anti Human CTNF antibody recognizes human CNTF (ciliary neurotrophic factor), a 200 amino acid homodimeric neurotrophic and differentiation factor of the CNTF family, which supports the survival and differentiation of several motor neuron types within the nervous system, but also affects other target tissues, including skeletal muscle (Vergara & Ramirez 2005), glial cells, hepatocytes and embryonic stem cells.

CNTF mainly signals through binding to a receptor complex consisting of a CNTFRa subunit, two beta components, gp130 and LIFR, resulting ultimately in the activation of JAK/STAT signalling pathway, but CNTF has also been shown to bind to the receptor for interleukin-6 (IL-6R).

Product Details

Target Species
Human
Product Form
Purified IgG - lyophilised
Reconstitution
Reconstitute with 0.1ml distilled water
Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution. For long term storage the addition of 0.09% sodium azide is recommended.
N.B. For functional studies do not add sodium azide
Antiserum Preparation
Antisera to human CNTF were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG prepared by affinity chromatography.
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
None present
Immunogen
Recombinant human CNTF (PHP173)
Approx. Protein Concentrations
IgG concentration 1.0mg/ml

Storage Information

Storage
Prior to reconstitution store at +4oC.
After reconstitution store at -20oC.
Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
12 months from date of reconstitution.

More Information

UniProt
P26441 Related reagents
Entrez Gene
CNTF Related reagents
GO Terms
GO:0005127 ciliary neurotrophic factor receptor binding
GO:0005138 interleukin-6 receptor binding
GO:0005615 extracellular space
GO:0040007 growth
GO:0008083 growth factor activity
GO:0042517 positive regulation of tyrosine phosphorylation of Stat3 protein
GO:0070120 ciliary neurotrophic factor-mediated signaling pathway
Regulatory
For research purposes only

Applications of CNTF antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 0.5 2.0ug/ml
Functional Assays 7.0 11.0ug/ml
Immunohistology - Paraffin 0.25ug/ml
Western Blotting 0.1 0.2ug/ml
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
ELISA
This purified human CNTF antibody may be used in an indirect ELISA or as a capture reagent in a sandwich ELISA with recombinant human CNTF (PHP173) as the standard and biotinylated human CNTF antibody (AHP1277B) as the detection reagent.
Western Blotting
This purified human CNTF antibody may be used in Western Blotting applications under either reducing or non-reducing conditions with recombinant human CNTF (PHP173) as the positive control.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Sheep anti Rabbit IgG:Biotin 2AB02B 1 ml C E P WB
Goat anti Rabbit IgG (Fc):Biotin STAR121B 1 mg E WB
Goat anti Rabbit IgG (Fc):FITC STAR121F 1 mg F
Goat anti Rabbit IgG (Fc):HRP STAR121P 1 mg E WB
Goat anti Rabbit IgG (H/L):HRP STAR124P 1 mg C E WB
Sheep anti Rabbit IgG:FITC STAR34B 1 mg C F
Sheep anti Rabbit IgG:RPE STAR35A 1 ml F
Sheep anti Rabbit IgG:DyLight®488 STAR36D488GA 0.1 mg F IF WB
Sheep anti Rabbit IgG:DyLight®549 STAR36D549GA 0.1 mg F IF
Sheep anti Rabbit IgG:DyLight®649 STAR36D649GA 0.1 mg F IF
Sheep anti Rabbit IgG:DyLight®680 STAR36D680GA 0.1 mg F IF WB
Sheep anti Rabbit IgG:Dylight®800 STAR36D800GA 0.1 mg F IF WB

Useful Reagents Available

Description Product Code Pack Size Applications List Price Quantity
Antigen Retrieval Buffer, pH8.0 BUF025A 500 ml P
Antigen Retrieval Buffer, pH8.0 BUF025C 50 ml P
TidyBlot™ Western Blot Detection Reagent:HRP STAR209P 0.5 ml WB*

Product Specific References

Further Reading

  1. Vergara, C. & Ramirez, B. (2004) CNTF, a pleiotropic cytokine: emphasis on its myotrophic role.
    Brain Res Rev. 47 (1-3): 161-73.