CNTF antibody


CNTF mainly signals through binding to a receptor complex consisting of a CNTFRa subunit, two beta components, gp130 and LIFR, resulting ultimately in the activation of JAK/STAT signalling pathway, but CNTF has also been shown to bind to the receptor for interleukin-6 (IL-6R).
Product Details
- Target Species
- Human
- Product Form
- Purified IgG - lyophilised
- Reconstitution
- Reconstitute with 0.1ml distilled water
Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution. For long term storage the addition of 0.09% sodium azide is recommended.
N.B. For functional studies do not add sodium azide - Antiserum Preparation
- Antisera to human CNTF were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG prepared by affinity chromatography.
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- None present
- Carrier Free
- Yes
- Immunogen
- Recombinant human CNTF (PHP173)
- Approx. Protein Concentrations
- IgG concentration 1.0mg/ml
Storage Information
- Storage
- Prior to reconstitution store at -20°C.
After reconstitution store at -20°C.
This product should be stored undiluted. Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Guarantee
- Guaranteed for 3 months from the date of reconstitution or until the date of expiry, whichever comes first. Please see label for expiry date.
More Information
- UniProt
- P26441
- Entrez Gene
- CNTF
- GO Terms
- GO:0005127 ciliary neurotrophic factor receptor binding
- GO:0005138 interleukin-6 receptor binding
- GO:0005615 extracellular space
- GO:0040007 growth
- GO:0008083 growth factor activity
- GO:0042517 positive regulation of tyrosine phosphorylation of Stat3 protein
- GO:0070120 ciliary neurotrophic factor-mediated signaling pathway
- Regulatory
- For research purposes only
Applications of CNTF antibody
Application Name | Verified | Min Dilution | Max Dilution |
---|---|---|---|
ELISA | 0.5 | 2.0ug/ml | |
Functional Assays | 7.0 | 11.0ug/ml | |
Immunohistology - Paraffin | 0.25ug/ml | ||
Western Blotting | 0.1 | 0.2ug/ml |
- ELISA
- This purified human CNTF antibody may be used in an indirect ELISA with recombinant human CNTF (PHP173) as the standard.
- Western Blotting
- This purified human CNTF antibody may be used in Western Blotting applications under either reducing or non-reducing conditions with recombinant human CNTF (PHP173) as the positive control.
Secondary Antibodies Available
Description | Product Code | Applications | Pack Size | List Price | Quantity |
---|---|---|---|---|---|
Goat anti Rabbit IgG (Fc):Biotin | STAR121B | E WB | 1 mg |
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Goat anti Rabbit IgG (Fc):FITC | STAR121F | F | 1 mg |
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Goat anti Rabbit IgG (Fc):HRP | STAR121P | E WB | 1 mg |
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Goat anti Rabbit IgG (H/L):HRP | STAR124P | C E WB | 1 mg |
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Sheep anti Rabbit IgG:FITC | STAR34B | C F | 1 mg |
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Sheep anti Rabbit IgG:RPE | STAR35A | F | 1 ml |
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Sheep anti Rabbit IgG:DyLight®488 | STAR36D488GA | F IF WB | 0.1 mg | ||
Sheep anti Rabbit IgG:DyLight®680 | STAR36D680GA | F IF WB | 0.1 mg | ||
Sheep anti Rabbit IgG:Dylight®800 | STAR36D800GA | F IF WB | 0.1 mg |
Useful Reagents Available
Description | Product Code | Applications | Pack Size | List Price | Quantity |
---|---|---|---|---|---|
Antigen Retrieval Buffer, pH8.0 | BUF025A | P | 500 ml | ||
Antigen Retrieval Buffer, pH8.0 | BUF025C | P | 50 ml | ||
TidyBlot Western Blot Detection Reagent:HRP | STAR209P | WB* | 0.5 ml |
Product Specific References
Further Reading
-
Vergara, C. & Ramirez, B. (2004) CNTF, a pleiotropic cytokine: emphasis on its myotrophic role.
Brain Res Rev. 47 (1-3): 161-73.
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