CNTF antibody

Product Code Applications Pack Size List Price Your Price Qty
AHP1277
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
E FN P WB 0.1 mg loader
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loader

Rabbit anti Human CTNF antibody recognizes human CNTF (ciliary neurotrophic factor), a 200 amino acid homodimeric neurotrophic and differentiation factor of the CNTF family, which supports the survival and differentiation of several motor neuron types within the nervous system, but also affects other target tissues, including skeletal muscle (Vergara & Ramirez 2005), glial cells, hepatocytes and embryonic stem cells.

CNTF mainly signals through binding to a receptor complex consisting of a CNTFRa subunit, two beta components, gp130 and LIFR, resulting ultimately in the activation of JAK/STAT signalling pathway, but CNTF has also been shown to bind to the receptor for interleukin-6 (IL-6R).

Target Species
Human
Product Form
Purified IgG - lyophilized
Reconstitution
Reconstitute with 0.1ml distilled water
Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution. For long term storage the addition of 0.09% sodium azide is recommended.
N.B. For functional studies do not add sodium azide
Antiserum Preparation
Antisera to human CNTF were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG prepared by affinity chromatography.
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
None present
Carrier Free
Yes
Immunogen
Recombinant human CNTF (PHP173)
Approx. Protein Concentrations
IgG concentration 1.0mg/ml
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

Prior to reconstitution store at -20°C.
After reconstitution store at -20°C.

This product should be stored undiluted. Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 0.5 2.0ug/ml
Functional Assays 7.0 11.0ug/ml
Immunohistology - Paraffin 0.25ug/ml
Western Blotting 0.1 0.2ug/ml
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
ELISA
This purified human CNTF antibody may be used in an indirect ELISA with recombinant human CNTF (PHP173) as the standard.
Western Blotting
This purified human CNTF antibody may be used in Western Blotting applications under either reducing or non-reducing conditions with recombinant human CNTF (PHP173) as the positive control.

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Further Reading

  1. Vergara, C. & Ramirez, B. (2004) CNTF, a pleiotropic cytokine: emphasis on its myotrophic role.
    Brain Res Rev. 47 (1-3): 161-73.

Synonyms
Ciliary Neurotrophic Factor
RRID
AB_2292055
UniProt
P26441
Entrez Gene
CNTF
GO Terms
GO:0005127 ciliary neurotrophic factor receptor binding
GO:0005138 interleukin-6 receptor binding
GO:0005615 extracellular space
GO:0040007 growth
GO:0008083 growth factor activity
GO:0042517 positive regulation of tyrosine phosphorylation of Stat3 protein
GO:0070120 ciliary neurotrophic factor-mediated signaling pathway

AHP1277

149045 170505

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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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