Clathrin Light Chain A/B antibody

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Goat anti Clathrin Light Chain A/B

Product Type
PrecisionAb Polyclonal
Isotype
Polyclonal IgG
Format
Purified
Product Code Applications Pack Size List Price Quantity
VPA00138
Datasheet
WB
MSDS
100 µl

Goat anti Human clathrin light chain A/B antibody recognizes the clathrin light chain A and B

Clathrin is a large, soluble protein composed of heavy and light chains. It functions as the main structural component of the lattice-type cytoplasmic face of coated pits and vesicles which entrap specific macromolecules during receptor-mediated endocytosis. CTLA encodes one of two clathrin light chain proteins which are believed to function as regulatory elements, CTLB encodes the other. Alternative splicing results in multiple transcript variants. Related pseudogenes have been identified on chromosomes 8 and 12 (provided by RefSeq, May 2010).

Goat anti Human clathrin light chain A/B detects a band of 27 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.

Product Details

Target Species
Human
Western Blotting
Anti clathrin light chain A/B detects a band of approximately 27 kDa in HepG2 cell lysates.
Product Form
Purified IgG - liquid
Preparation
Goat polyclonal antibody purified by affinity chromatography.
Buffer Solution
TRIS buffered saline
Preservative Stabilisers
0.02% Sodium Azide (NaN3) 0.5 % BSA
Immunogen
Peptide with the sequence CDFNPKSSKQAKD, from the internal region of the protein sequence.

Storage Information

Storage
Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
Guarantee
12 months from date of despatch

More Information

UniProt
P09496
P09497
Entrez Gene
CLTA
CLTB
GO Terms
GO:0005829 cytosol
GO:0005198 structural molecule activity
GO:0006886 intracellular protein transport
GO:0006892 post-Golgi vesicle-mediated transport
GO:0007173 epidermal growth factor receptor signaling pathway
GO:0007411 axon guidance
GO:0030130 clathrin coat of trans-Golgi network vesicle
GO:0030132 clathrin coat of coated pit
GO:0042059 negative regulation of epidermal growth factor receptor signaling pathway
GO:0048011 nerve growth factor receptor signaling pathway
GO:0005515 protein binding
GO:0016192 vesicle-mediated transport
Acknowledgements
PrecisionAb is a trademark of Bio-Rad Laboratories.
Regulatory
For research purposes only

Applications of Clathrin Light Chain A/B antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000

The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Donkey anti Sheep/Goat IgG:HRP STAR88P C E P WB 1 ml loader