Clathrin Light Chain A/B antibody

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Goat anti Clathrin Light Chain A/B

Clathrin light chain A/B antibody (CTLA + CTLB) recognizes the clathrin light chain A and B, which detects a band of 27 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.

Clathrin Light Chain A/B Antibody with Control Lysate

Clathrin light chain A/B antibody (CTLA + CTLB) recognizes the clathrin light chain A and B, which detects a band of 27 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.

Product Type
PrecisionAb™ Polyclonal
Isotype
Polyclonal IgG
Format
Purified

Select your package type:

Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
VPA00138 WB 100 µl
VPA00138KT WB 2 Westerns
With Lysate 400µg
Goat anti Human clathrin light chain A/B antibody recognizes the clathrin light chain A and B

Clathrin is a large, soluble protein composed of heavy and light chains. It functions as the main structural component of the lattice-type cytoplasmic face of coated pits and vesicles which entrap specific macromolecules during receptor-mediated endocytosis. CTLA encodes one of two clathrin light chain proteins which are believed to function as regulatory elements, CTLB encodes the other. Alternative splicing results in multiple transcript variants. Related pseudogenes have been identified on chromosomes 8 and 12 (provided by RefSeq, May 2010).

Goat anti Human clathrin light chain A/B detects a band of 27 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.

Product Details

Target Species
Human
Western Blotting
Anti clathrin light chain A/B detects a band of approximately 27 kDa in HepG2 cell lysates.
Product Form
Purified IgG - liquid
Product Form
Purified IgG - liquid
Preparation
Goat polyclonal antibody purified by affinity chromatography.
Preparation
20μl Goat polyclonal antibody purified by affinity chromatography
Buffer Solution
TRIS buffered saline
Buffer Solution
TRIS buffered saline
Preservative Stabilisers
0.02% Sodium Azide (NaN3) 0.5 % BSA
Preservative Stabilisers
0.02% Sodium Azide (NaN3) 0.5 % BSA
Immunogen
Peptide with the sequence CDFNPKSSKQAKD, from the internal region of the protein sequence.
Instructions For Use
Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.

Positive Control Lysate

Lysate Composition
400μg HepG2 lysate lyophilized in RIPA buffer.
Lysate Reconstitution
- If using DDT reconstitute the lyophilized lysate with 190μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 10μl 2M DTT.
- If using BME reconstitute the lyophilized lysate with 180μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 20μl BME.
Heat at 95°C for 5 minutes. For 10 well mini gels load 25μl. For other gel and comb formats please refer to the PrecisionAb western blotting protocol.

Storage Information

Storage
Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
Storage
Antibody: Store undiluted at -20oC, avoiding repeated freeze thaw cycles.

Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage.
Shelf Life
As supplied, 12 months from date of despatch.
Shelf Life
As supplied, 12 months from date of despatch.

More Information

UniProt
P09496 Related reagents
P09497 Related reagents
Entrez Gene
CLTA Related reagents
CLTB Related reagents
GO Terms
GO:0005829 cytosol
GO:0005198 structural molecule activity
GO:0006886 intracellular protein transport
GO:0006892 post-Golgi vesicle-mediated transport
GO:0007173 epidermal growth factor receptor signaling pathway
GO:0007411 axon guidance
GO:0030130 clathrin coat of trans-Golgi network vesicle
GO:0030132 clathrin coat of coated pit
GO:0042059 negative regulation of epidermal growth factor receptor signaling pathway
GO:0048011 nerve growth factor receptor signaling pathway
GO:0005515 protein binding
GO:0016192 vesicle-mediated transport
Acknowledgements
PrecisionAb™ is a trademark of Bio-Rad Laboratories.
Acknowledgements
PrecisionAb™ is a trademark of Bio-Rad Laboratories.
Regulatory
For research purposes only

Applications of Clathrin Light Chain A/B antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000
Western Blotting 1/1000

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Donkey anti Sheep/Goat IgG:HRP STAR88P 1 ml C E P WB
Donkey anti Sheep/Goat IgG:HRP STAR88P 1 ml C E P WB

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