CHRNA5 antibody

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Rabbit anti CHRNA5

Product Type
PrecisionAb Polyclonal
Isotype
Polyclonal IgG
Format
Purified
Product Code Applications Pack Size List Price Quantity
VPA00527
Datasheet
WB
MSDS
100 µl loader

Rabbit anti Human CHRNA5 antibody recognizes CHRNA5 also known as neuronal acetylcholine receptor subunit alpha-5, Cholinergic receptor, neuronal nicotinic, alpha polypeptide-5, or neuronal nicotinic acetylcholine receptor, alpha5 subunit.

The protein encoded by the CHRNA5 gene is a nicotinic acetylcholine receptor subunit and a member of a superfamily of ligand-gated ion channels that mediate fast signal transmission at synapses. These receptors are thought to be heteropentamers composed of separate but similar subunits. Defects in CHRNA5 have been linked to susceptibility to lung cancer type 2 (LNCR2) (provided by RefSeq, Jun 2010).

Rabbit anti Human CHRNA5 antibody detects a band of 53 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.

Product Details

Target Species
Human
Western Blotting
Anti CHRNA5 detects a band of approximately 53 kDa in A431 cell lysates.
Species Cross-Reactivity
Target SpeciesCross Reactivity
Rat
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid.
Preparation
Rabbit polyclonal antibody prepared by affinity chromatography on Protein A.
Buffer Solution
Phosphate buffered saline.
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
2% Sucrose.
Immunogen
Synthetic peptide directed towards the middle region of human CHRNA5

Storage Information

Storage
Store undiluted at -20°C, avoiding repeated freeze thaw cycles
Guarantee
12 months from date of despatch

More Information

UniProt
P30532
Entrez Gene
CHRNA5
GO Terms
GO:0004889 nicotinic acetylcholine-activated cation-selective channel activity
GO:0005892 nicotinic acetylcholine-gated receptor-channel complex
GO:0015464 acetylcholine receptor activity
GO:0030054 cell junction
GO:0035095 behavioral response to nicotine
GO:0045211 postsynaptic membrane
Acknowledgements
PrecisionAb is a trademark of Bio-Rad Laboratories
Regulatory
For research purposes only.

Applications of CHRNA5 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000

The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Goat anti Rabbit IgG (H/L):HRP STAR208P WB 2 ml