Chondroitin Sulphate Proteoglycan NG2 antibody

Rabbit anti Chondroitin Sulphate Proteoglycan NG2

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Specificity
Chondroitin Sulphate Proteoglycan NG2

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AHP2741
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SDS Safety Datasheet SDS
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Rabbit anti chondroitin sulphate proteoglycan NG2 antibody recognizes chondroitin sulfate proteoglycan NG2 also known as chondroitin sulfate proteoglycan 4 (CSPG4) and melanoma chondroitin sulfate proteoglycan. It was first characterized in rat as a high-molecular-weight type 1 membrane proteoglycan (Stallcup 1981). CSPG4 is involved in cell attachment and migration (Karram et al. 2005). It regulates spreading of melanoma cells by recruiting and activating a signalling cascade through CDC42, ACK1 and p130cas (Eisenmann et al. 1999). It enhances FAK and ERK activation (Yang et al. 2004).

Target Species
Human
Product Form
Purified IgG - liquid
Antiserum Preparation
Antiserum to chondroitin sulphate proteoglycan NG2 was raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.02% Sodium Azide
50% Glycerol
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1950-2210 of human chondroitin sulphate proteoglycan NG2
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

Store at -20oC
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/500 1/2000
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Western Blotting
Rabbit anti chondroitin sulphate proteoglycan NG2 antibody detects a band of approximately 320 kDa in HeLa and A375 cell lysates under reducing conditions

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UniProt
Q6UVK1
Entrez Gene
CSPG4
GO Terms
GO:0001525 angiogenesis
GO:0004871 signal transducer activity
GO:0035556 intracellular signal transduction
GO:0005887 integral to plasma membrane
GO:0009986 cell surface
GO:0016324 apical plasma membrane
GO:0019901 protein kinase binding
GO:0030154 cell differentiation
GO:0031258 lamellipodium membrane
GO:0048771 tissue remodeling
GO:0050731 positive regulation of peptidyl-tyrosine phosphorylation
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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