Chondroitin Sulphate Proteoglycan NG2 antibody
Rabbit anti chondroitin sulphate proteoglycan NG2 antibody recognizes chondroitin sulfate proteoglycan NG2 also known as chondroitin sulfate proteoglycan 4 (CSPG4) and melanoma chondroitin sulfate proteoglycan. It was first characterized in rat as a high-molecular-weight type 1 membrane proteoglycan (Stallcup 1981). CSPG4 is involved in cell attachment and migration (Karram et al. 2005). It regulates spreading of melanoma cells by recruiting and activating a signalling cascade through CDC42, ACK1 and p130cas (Eisenmann et al. 1999). It enhances FAK and ERK activation (Yang et al. 2004).
Product Details
- Target Species
- Human
- Product Form
- Purified IgG - liquid
- Antiserum Preparation
- Antiserum to chondroitin sulphate proteoglycan NG2 was raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.02% Sodium Azide
50% Glycerol - Immunogen
- Recombinant fusion protein containing a sequence corresponding to amino acids 1950-2210 of human chondroitin sulphate proteoglycan NG2
Storage Information
- Storage
- Store at -20oC
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Guarantee
- 12 months from date of despatch
More Information
- UniProt
- Q6UVK1
- Entrez Gene
- CSPG4
- GO Terms
- GO:0001525 angiogenesis
- GO:0004871 signal transducer activity
- GO:0035556 intracellular signal transduction
- GO:0005887 integral to plasma membrane
- GO:0009986 cell surface
- GO:0016324 apical plasma membrane
- GO:0019901 protein kinase binding
- GO:0030154 cell differentiation
- GO:0031258 lamellipodium membrane
- GO:0048771 tissue remodeling
- GO:0050731 positive regulation of peptidyl-tyrosine phosphorylation
- Regulatory
- For research purposes only
Applications of Chondroitin Sulphate Proteoglycan NG2 antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Western Blotting | 1/500 | 1/2000 |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Western Blotting
- Rabbit anti chondroitin sulphate proteoglycan NG2 antibody detects a band of approximately 320 kDa in HeLa and A375 cell lysates under reducing conditions
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Secondary Antibodies Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| Goat anti Rabbit IgG (Fc):Biotin | STAR121B | E WB | 1 mg |
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| Goat anti Rabbit IgG (Fc):FITC | STAR121F | F | 1 mg |
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| Goat anti Rabbit IgG (Fc):HRP | STAR121P | E WB | 1 mg |
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| Goat anti Rabbit IgG (H/L):HRP | STAR124P | C E WB | 1 mg |
|
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| Sheep anti Rabbit IgG:FITC | STAR34B | C F | 1 mg |
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| Sheep anti Rabbit IgG:RPE | STAR35A | F | 1 ml |
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