Cholesterol 24-Hydroxylase antibody

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Goat anti Cholesterol 24-Hydroxylase (C-Terminal)

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
AHP1254 E WB 0.1 ml
Goat anti Cholesterol 24 hydroxylase antibody recognizes cholesterol 24-hydroxylase (CYP46A1), a ~58 kDa member of the Cytochrome P450 family, expressed in the brain. CYP46A1 converts cholesterol into the biologically active oxysterol and 24(S)-hydroxycholesterol, in the first step of the enzymatic degradation of cholesterol in the brain. CYP46A1 is also thought to play a role in the metabolism of neurosteroids and drugs that can cross the blood-brain barrier and are targeted to the central nervous system.

P450 enzymes are divided into two groups: steroidogenic and xenobiotic. The latter group is comprised of three families 1, 2 and 3. The xenobiotic p450's are involved in most oxidative drug metabolism.

Product Details

Target Species
Human
Product Form
Serum - liquid
Antiserum Preparation
Antisera to cholesterol 24-hydroxylase (CT) were raised by repeated immunisations of goats with highly purified antigen.
Preservative Stabilisers
0.09%Sodium Azide (NaN3)
Immunogen
Synthetic peptide corresponding to amino acid sequence 254-270 of human cholesterol 24-hydroxylase protein.

Storage Information

Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.

More Information

UniProt
Q9Y6A2 Related reagents
Entrez Gene
CYP46A1 Related reagents
GO Terms
GO:0009055 electron carrier activity
GO:0016021 integral to membrane
GO:0005789 endoplasmic reticulum membrane
GO:0005792 microsome
GO:0055114 oxidation-reduction process
GO:0006699 bile acid biosynthetic process
GO:0006707 cholesterol catabolic process
GO:0006805 xenobiotic metabolic process
GO:0007399 nervous system development
GO:0008395 steroid hydroxylase activity
GO:0020037 heme binding
GO:0033781 cholesterol 24-hydroxylase activity
Regulatory
For research purposes only

Applications of Cholesterol 24-Hydroxylase antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 1/15000
Western Blotting 1/3000
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Western Blotting
AHP1254 detects a band of approximately 58kDa in human Alzheimer diseased brain lysates.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Rabbit anti Goat IgG (Fc):FITC STAR122F 1 mg F
Rabbit anti Goat IgG (Fc):HRP STAR122P 1 mg C E WB

Product Specific References

References for Cholesterol 24-Hydroxylase antibody

  1. Pikuleva, I.A. (2006) Cytochrome P450s and cholesterol homeostasis.
    Pharmacol Ther. 112 (3): 761-73.
  2. Mast, N. et al. (2003) Broad substrate specificity of human cytochrome P450 46A1 which initiates cholesterol degradation in the brain.
    Biochemistry. 42 (48): 14284-92.
  3. Ng, P.S. et al. (2003) Production of inhibitory polyclonal antibodies against cytochrome P450s.
    Drug Metab Pharmacokinet. 18 (3): 163-72.