Rabbit anti Chk2 (pSer516) antibody
recognizes Chk2, also known as serine/threonine-protein kinase Chk2, CHK2 checkpoint homolog, Cds1 homolog (hCds1) and checkpoint kinase 2, when phosphorylated at serine 516. Chk2 is a serine/threonine-protein kinase involved in cell cycle arrest, activation of DNA repair and apoptosis.
Activated Chk2 phosphorylates and inhibits Cdc25C phosphatase, stabilizes the tumor suppressor protein p53 leading to cell cycle arrest in G1. In addition, Chk2 can phosphorylate BRCA1, activating cell survival pathways.
Chk2 is a putative tumor suppressor protein, with mutations of the Chk2 gene linked to the familial cancer Li-Fraumeni syndrome. Autophosphorylation of Chk2 at serine 516 is important for full activation of Chk2 (Wu and Chen 2003
- Target Species
- Product Form
- Purified IgG - liquid
- Antiserum Preparation
- Antiserum to Chk2 (pSer516) was raised by repeated immunization of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.02% Sodium Azide
- Phospho specific-peptide corresponding to residues surrounding serine 516 of human Chk2
- Store at -20oC
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
- 12 months from date of despatch
- Entrez Gene
- For research purposes only
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Applications of Chk2 (pSer516) antibody
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Copyright © 2021 Bio-Rad Antibodies (formerly AbD Serotec)
Secondary Antibodies Available
Useful Reagents Available
Product Specific References
Wu, X. & Chen, J. (2003) Autophosphorylation of checkpoint kinase 2 at serine 516 is required for radiation-induced apoptosis.
J Biol Chem. 278 (38): 36163-8.
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