Chk2 (pSer516) antibody

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Rabbit anti Chk2 (pSer516)

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
AHP2574 WB 50 µl
Rabbit anti Chk2 (pSer516) antibody recognizes Chk2, also known as serine/threonine-protein kinase Chk2, CHK2 checkpoint homolog, Cds1 homolog (hCds1) and checkpoint kinase 2, when phosphorylated at serine 516. Chk2 is a serine/threonine-protein kinase involved in cell cycle arrest, activation of DNA repair and apoptosis.

Activated Chk2 phosphorylates and inhibits Cdc25C phosphatase, stabilizes the tumor suppressor protein p53 leading to cell cycle arrest in G1. In addition, Chk2 can phosphorylate BRCA1, activating cell survival pathways.

Chk2 is a putative tumor suppressor protein, with mutations of the Chk2 gene linked to the familial cancer Li-Fraumeni syndrome. Autophosphorylation of Chk2 at serine 516 is important for full activation of Chk2 (Wu and Chen 2003).

Product Details

Target Species
Human
Product Form
Purified IgG - liquid
Antiserum Preparation
Antiserum to Chk2 (pSer516) was raised by repeated immunization of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.02% Sodium Azide
50% Glycerol
Immunogen
Phospho specific-peptide corresponding to residues surrounding serine 516 of human Chk2

Storage Information

Storage
Store at -20oC
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
12 months from date of despatch

More Information

UniProt
O96017 Related reagents
Entrez Gene
CHEK2 Related reagents
Regulatory
For research purposes only

Applications of Chk2 (pSer516) antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/500 1/2000
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Sheep anti Rabbit IgG:Biotin 2AB02B 1 ml C E P WB
Goat anti Rabbit IgG (Fc):Biotin STAR121B 1 mg E WB
Goat anti Rabbit IgG (Fc):FITC STAR121F 1 mg F
Goat anti Rabbit IgG (Fc):HRP STAR121P 1 mg E WB
Goat anti Rabbit IgG (H/L):HRP STAR124P 1 mg C E WB
Sheep anti Rabbit IgG:FITC STAR34B 1 mg C F
Sheep anti Rabbit IgG:RPE STAR35A 1 ml F
Sheep anti Rabbit IgG:DyLight®488 STAR36D488GA 0.1 mg F IF WB
Sheep anti Rabbit IgG:DyLight®549 STAR36D549GA 0.1 mg F IF
Sheep anti Rabbit IgG:DyLight®649 STAR36D649GA 0.1 mg F IF
Sheep anti Rabbit IgG:DyLight®680 STAR36D680GA 0.1 mg F IF WB
Sheep anti Rabbit IgG:Dylight®800 STAR36D800GA 0.1 mg F IF WB

Useful Reagents Available

Description Product Code Pack Size Applications List Price Quantity
TidyBlot Western Blot Detection Reagent:HRP STAR209P 0.5 ml WB*

Product Specific References

Further Reading

  1. Wu, X. & Chen, J. (2003) Autophosphorylation of checkpoint kinase 2 at serine 516 is required for radiation-induced apoptosis.
    J Biol Chem. 278 (38): 36163-8.