In response to DNA damage and replication blocks, cell cycle progression is halted through the control of critical cell cycle regulators. The protein encoded by this gene is a cell cycle checkpoint regulator and putative tumor suppressor. It contains a forkhead-associated protein interaction domain essential for activation in response to DNA damage and is rapidly phosphorylated in response to replication blocks and DNA damage. When activated, the encoded protein is known to inhibit CDC25C phosphatase, preventing entry into mitosis, and has been shown to stabilize the tumor suppressor protein p53, leading to cell cycle arrest in G1. In addition, this protein interacts with and phosphorylates BRCA1, allowing BRCA1 to restore survival after DNA damage. Mutations in this gene have been linked with Li-Fraumeni syndrome, a highly penetrant familial cancer phenotype usually associated with inherited mutations in TP53. Also, mutations in this gene are thought to confer a predisposition to sarcomas, breast cancer, and brain tumors. This nuclear protein is a member of the CDS1 subfamily of serine/threonine protein kinases. Several transcript variants encoding different isoforms have been found for this gene (provided by RefSeq, Apr 2012).
Rabbit anti Human CHK2 antibody detects a band of 62 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.
- Target Species
- Western Blotting
- Anti CHK2 antibody recognizes a band of approximately 62 kDa in HCT116 cell lysates
- Product Form
- Purified IgG - liquid
- Rabbit polyclonal antibody purified by affinity chromatography on immunogen
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
- KLH conjugated synthetic peptide corresponding to a portion of the N terminal region of human CHK2
- Store undiluted at -20oC, avoiding repeated freeze thaw cycles
- 12 months from date of despatch
- PrecisionAb is a trademark of Bio-Rad Laboratories
- For research purposes only
Applications of CHK2 antibody
|Application Name||Verified||Min Dilution||Max Dilution|
The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.
Secondary Antibodies Available
|Description||Product Code||Applications||Pack Size||List Price||Quantity|
|Goat anti Rabbit IgG (H/L):HRP||STAR208P||WB||2 ml|