Rabbit anti Chk1 (pSer280) antibody
recognizes Chk1 also known as checkpoint kinase-1, CHK1 checkpoint homolog or cell cycle checkpoint kinase, when phosphorylated at serine 280.
Chk1 is a serine/threonine protein kinase that plays a crucial role in cell cycle control. Chk1 is involved in triggering cell cycle arrest, DNA repair and apoptosis (Bartek and Lukas 2003
Chk1 plays a critical role in preventing aberrant cells from progressing through the cell cycle (Thompson and Eastman 2013
). Chk1 is phosphorylated at serine 280 by p90 RSK (Li et al. 2012
) resulting in nuclear accumulation of Chk1.
- Target Species
- Product Form
- Purified IgG - liquid
- Antiserum Preparation
- Antiserum to Chk1 (pSer280) was raised by repeated immunization of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.02% Sodium Azide
- Phospho specific-peptide corresponding to residues surrounding serine 280 of human Chk1
- Store at -20oC
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
- 12 months from date of despatch
- Entrez Gene
- GO Terms
DNA damage checkpoint
regulation of cyclin-dependent protein kinase activity
condensed nuclear chromosome
protein serine/threonine kinase activity
microtubule organizing center
reciprocal meiotic recombination
negative regulation of cell proliferation
- For research purposes only
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Applications of Chk1 (pSer280) antibody
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Copyright © 2021 Bio-Rad Antibodies (formerly AbD Serotec)
Secondary Antibodies Available
Useful Reagents Available
Product Specific References
Bartek, J. & Lukas, J. (2003) Chk1 and Chk2 kinases in checkpoint control and cancer.
Cancer Cell. 3 (5): 421-9.
Thompson, R. & Eastman, A. (2013) The cancer therapeutic potential of Chk1 inhibitors: how mechanistic studies impact on clinical trial design.
Br J Clin Pharmacol. 76 (3): 358-69.
Li, P. et al. (2012) P90 RSK arranges Chk1 in the nucleus for monitoring of genomic integrity during cell proliferation.
Mol Biol Cell. 23 (8): 1582-92.
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