Chk1 (pSer280) antibody

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Rabbit anti Chk1 (pSer280)

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
AHP2573 WB 50 µl
Rabbit anti Chk1 (pSer280) antibody recognizes Chk1 also known as checkpoint kinase-1, CHK1 checkpoint homolog or cell cycle checkpoint kinase, when phosphorylated at serine 280.

Chk1 is a serine/threonine protein kinase that plays a crucial role in cell cycle control. Chk1 is involved in triggering cell cycle arrest, DNA repair and apoptosis (Bartek and Lukas 2003).

Chk1 plays a critical role in preventing aberrant cells from progressing through the cell cycle (Thompson and Eastman 2013). Chk1 is phosphorylated at serine 280 by p90 RSK (Li et al. 2012) resulting in nuclear accumulation of Chk1.

Product Details

Target Species
Human
Product Form
Purified IgG - liquid
Antiserum Preparation
Antiserum to Chk1 (pSer280) was raised by repeated immunization of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.02% Sodium Azide
50% Glycerol
Immunogen
Phospho specific-peptide corresponding to residues surrounding serine 280 of human Chk1

Storage Information

Storage
Store at -20oC
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
12 months from date of despatch

More Information

UniProt
O14757 Related reagents
Entrez Gene
CHEK1 Related reagents
GO Terms
GO:0006260 DNA replication
GO:0000077 DNA damage checkpoint
GO:0000079 regulation of cyclin-dependent protein kinase activity
GO:0005515 protein binding
GO:0000794 condensed nuclear chromosome
GO:0004674 protein serine/threonine kinase activity
GO:0005524 ATP binding
GO:0005654 nucleoplasm
GO:0005815 microtubule organizing center
GO:0006281 DNA repair
GO:0006468 protein phosphorylation
GO:0007131 reciprocal meiotic recombination
GO:0007276 gamete generation
GO:0008285 negative regulation of cell proliferation
GO:0090399 replicative senescence
Regulatory
For research purposes only

Applications of Chk1 (pSer280) antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/500 1/2000
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Western Blotting
Due to the presence of phosphoproteins in milk, the use of milk based blocking reagents is not recommended. 1% BSA in PBS or TBS Tween should be used instead.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Sheep anti Rabbit IgG:Biotin 2AB02B 1 ml C E P WB
Goat anti Rabbit IgG (Fc):Biotin STAR121B 1 mg E WB
Goat anti Rabbit IgG (Fc):FITC STAR121F 1 mg F
Goat anti Rabbit IgG (Fc):HRP STAR121P 1 mg E WB
Goat anti Rabbit IgG (H/L):HRP STAR124P 1 mg C E WB
Sheep anti Rabbit IgG:FITC STAR34B 1 mg C F
Sheep anti Rabbit IgG:RPE STAR35A 1 ml F
Sheep anti Rabbit IgG:DyLight®488 STAR36D488GA 0.1 mg F IF WB
Sheep anti Rabbit IgG:DyLight®549 STAR36D549GA 0.1 mg F IF
Sheep anti Rabbit IgG:DyLight®649 STAR36D649GA 0.1 mg F IF
Sheep anti Rabbit IgG:DyLight®680 STAR36D680GA 0.1 mg F IF WB
Sheep anti Rabbit IgG:Dylight®800 STAR36D800GA 0.1 mg F IF WB

Useful Reagents Available

Description Product Code Pack Size Applications List Price Quantity
TidyBlot™ Western Blot Detection Reagent:HRP STAR209P 0.5 ml WB*

Product Specific References

Further Reading

  1. Bartek, J. & Lukas, J. (2003) Chk1 and Chk2 kinases in checkpoint control and cancer.
    Cancer Cell. 3 (5): 421-9.
  2. Thompson, R. & Eastman, A. (2013) The cancer therapeutic potential of Chk1 inhibitors: how mechanistic studies impact on clinical trial design.
    Br J Clin Pharmacol. 76 (3): 358-69.
  3. Li, P. et al. (2012) P90 RSK arranges Chk1 in the nucleus for monitoring of genomic integrity during cell proliferation.
    Mol Biol Cell. 23 (8): 1582-92.