Chk1 (pSer280) antibody
Rabbit anti Chk1 (pSer280) antibody recognizes Chk1 also known as checkpoint kinase-1, CHK1 checkpoint homolog or cell cycle checkpoint kinase, when phosphorylated at serine 280.
Chk1 is a serine/threonine protein kinase that plays a crucial role in cell cycle control. Chk1 is involved in triggering cell cycle arrest, DNA repair and apoptosis (Bartek and Lukas 2003).
Chk1 plays a critical role in preventing aberrant cells from progressing through the cell cycle (Thompson and Eastman 2013). Chk1 is phosphorylated at serine 280 by p90 RSK (Li et al. 2012) resulting in nuclear accumulation of Chk1.
Chk1 is a serine/threonine protein kinase that plays a crucial role in cell cycle control. Chk1 is involved in triggering cell cycle arrest, DNA repair and apoptosis (Bartek and Lukas 2003).
Chk1 plays a critical role in preventing aberrant cells from progressing through the cell cycle (Thompson and Eastman 2013). Chk1 is phosphorylated at serine 280 by p90 RSK (Li et al. 2012) resulting in nuclear accumulation of Chk1.
Product Details
- Target Species
- Human
- Product Form
- Purified IgG - liquid
- Antiserum Preparation
- Antiserum to Chk1 (pSer280) was raised by repeated immunization of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.02% Sodium Azide
50% Glycerol - Immunogen
- Phospho specific-peptide corresponding to residues surrounding serine 280 of human Chk1
Storage Information
- Storage
- Store at -20oC
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Shelf Life
- 12 months from date of despatch
More Information
- UniProt
- O14757 Related reagents
- Entrez Gene
- CHEK1 Related reagents
- GO Terms
- GO:0006260 DNA replication
- GO:0000077 DNA damage checkpoint
- GO:0000079 regulation of cyclin-dependent protein kinase activity
- GO:0005515 protein binding
- GO:0000794 condensed nuclear chromosome
- GO:0004674 protein serine/threonine kinase activity
- GO:0005524 ATP binding
- GO:0005654 nucleoplasm
- GO:0005815 microtubule organizing center
- GO:0006281 DNA repair
- GO:0006468 protein phosphorylation
- GO:0007131 reciprocal meiotic recombination
- GO:0007276 gamete generation
- GO:0008285 negative regulation of cell proliferation
- GO:0090399 replicative senescence
- Regulatory
- For research purposes only
Applications of Chk1 (pSer280) antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Western Blotting | 1/500 | 1/2000 |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Western Blotting
- Due to the presence of phosphoproteins in milk, the use of milk based blocking reagents is not recommended. 1% BSA in PBS or TBS Tween should be used instead.
Copyright © 2018 Bio-Rad Antibodies (formerly AbD Serotec)
Secondary Antibodies Available
| Description | Product Code | Pack Size | Applications | List Price | Quantity |
|---|---|---|---|---|---|
| Sheep anti Rabbit IgG:Biotin | 2AB02B | 1 ml | C E P WB | ||
| Goat anti Rabbit IgG (Fc):Biotin | STAR121B | 1 mg | E WB | ||
| Goat anti Rabbit IgG (Fc):FITC | STAR121F | 1 mg | F | ||
| Goat anti Rabbit IgG (Fc):HRP | STAR121P | 1 mg | E WB | ||
| Goat anti Rabbit IgG (H/L):HRP | STAR124P | 1 mg | C E WB | ||
| Sheep anti Rabbit IgG:FITC | STAR34B | 1 mg | C F | ||
| Sheep anti Rabbit IgG:RPE | STAR35A | 1 ml | F | ||
| Sheep anti Rabbit IgG:DyLight®488 | STAR36D488GA | 0.1 mg | F IF WB | ||
| Sheep anti Rabbit IgG:DyLight®549 | STAR36D549GA | 0.1 mg | F IF | ||
| Sheep anti Rabbit IgG:DyLight®649 | STAR36D649GA | 0.1 mg | F IF | ||
| Sheep anti Rabbit IgG:DyLight®680 | STAR36D680GA | 0.1 mg | F IF WB | ||
| Sheep anti Rabbit IgG:Dylight®800 | STAR36D800GA | 0.1 mg | F IF WB |
Useful Reagents Available
| Description | Product Code | Pack Size | Applications | List Price | Quantity |
|---|---|---|---|---|---|
| TidyBlot™ Western Blot Detection Reagent:HRP | STAR209P | 0.5 ml | WB* |
Product Specific References
Further Reading
-
Bartek, J. & Lukas, J. (2003) Chk1 and Chk2 kinases in checkpoint control and cancer.
Cancer Cell. 3 (5): 421-9. -
Thompson, R. & Eastman, A. (2013) The cancer therapeutic potential of Chk1 inhibitors: how mechanistic studies impact on clinical trial design.
Br J Clin Pharmacol. 76 (3): 358-69. -
Li, P. et al. (2012) P90 RSK arranges Chk1 in the nucleus for monitoring of genomic integrity during cell proliferation.
Mol Biol Cell. 23 (8): 1582-92.
