Chk1 antibody
Rabbit anti Chk1
Rabbit anti-human CHK1 antibody recognizes serine/threonine-protein kinase CHK1, also known as CHK1 checkpoint homolog, cell cycle checkpoint kinase or checkpoint kinase-1. This antibody has been extensively validated for western blotting using whole cell lysates.
Chk1 Antibody with Control Lysate
Rabbit anti-human CHK1 antibody recognizes serine/threonine-protein kinase CHK1, also known as CHK1 checkpoint homolog, cell cycle checkpoint kinase or checkpoint kinase-1. This antibody has been extensively validated for western blotting using whole cell lysates.
- Product Type
- PrecisionAb™ Polyclonal
- Isotype
- Polyclonal IgG
- Format
- Purified
| Product Code | Applications | Datasheet | MSDS | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|---|
| VPA00572 | WB | 100 µl | ||||
| VPA00572KT | WB | 2 Westerns | ||||
| With Lysate | 400µg |
Rabbit anti Human Chk1 antibody recognizes Chk1 also known as checkpoint kinase-1, CHK1 checkpoint homolog or cell cycle checkpoint kinase .
The protein encoded by CHEK1 gene belongs to the Ser/Thr protein kinase family. It is required for checkpoint mediated cell cycle arrest in response to DNA damage or the presence of unreplicated DNA. This protein acts to integrate signals from ATM and ATR, two cell cycle proteins involved in DNA damage responses, that also associate with chromatin in meiotic prophase I. Phosphorylation of CDC25A protein phosphatase by this protein is required for cells to delay cell cycle progression in response to double-strand DNA breaks. Several alternatively spliced transcript variants have been found for CHEK1 (provided by RefSeq, Oct 2011).
Rabbit anti Human Chk1 antibody detects a band of 56 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.
The protein encoded by CHEK1 gene belongs to the Ser/Thr protein kinase family. It is required for checkpoint mediated cell cycle arrest in response to DNA damage or the presence of unreplicated DNA. This protein acts to integrate signals from ATM and ATR, two cell cycle proteins involved in DNA damage responses, that also associate with chromatin in meiotic prophase I. Phosphorylation of CDC25A protein phosphatase by this protein is required for cells to delay cell cycle progression in response to double-strand DNA breaks. Several alternatively spliced transcript variants have been found for CHEK1 (provided by RefSeq, Oct 2011).
Rabbit anti Human Chk1 antibody detects a band of 56 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.
Product Details
- Target Species
- Human
- Western Blotting
- Anti Chk1 detects a band of approximately 56 kDa in HeLa cell lysates
- Species Cross-Reactivity
-
Target Species Cross Reactivity Mouse Rat - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Product Form
- Purified IgG - liquid
- Preparation
- Rabbit polyclonal antibody purified by affinity chromatography
- Preparation
- 20μl Rabbit polyclonal antibody purified by affinity chromatography
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
- Immunogen
- KLH conjugated synthetic peptide corresponding to 258-287 amino acid from human Chk1
- Instructions For Use
- Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.
Positive Control Lysate
- Lysate Composition
- 400μg HeLa lysate lyophilized in RIPA buffer.
- Lysate Reconstitution
- - If using DDT reconstitute the lyophilized lysate with 190μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 10μl 2M DTT.
- If using BME reconstitute the lyophilized lysate with 180μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 20μl BME.
Heat at 95°C for 5 minutes. For 10 well mini gels load 25μl. For other gel and comb formats please refer to the PrecisionAb western blotting protocol.
Storage Information
- Storage
- Store undiluted at -20oC, avoiding repeated freeze thaw cycles
- Storage
- Antibody: Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage. - Shelf Life
- As supplied, 12 months from date of despatch
- Shelf Life
- As supplied, 12 months from date of despatch
More Information
- UniProt
- O14757 Related reagents
- Entrez Gene
- CHEK1 Related reagents
- GO Terms
- GO:0006260 DNA replication
- GO:0000077 DNA damage checkpoint
- GO:0000079 regulation of cyclin-dependent protein kinase activity
- GO:0005515 protein binding
- GO:0000794 condensed nuclear chromosome
- GO:0004674 protein serine/threonine kinase activity
- GO:0005524 ATP binding
- GO:0005654 nucleoplasm
- GO:0005815 microtubule organizing center
- GO:0006281 DNA repair
- GO:0006468 protein phosphorylation
- GO:0007131 reciprocal meiotic recombination
- GO:0007276 gamete generation
- GO:0008285 negative regulation of cell proliferation
- GO:0090399 replicative senescence
- Acknowledgements
- PrecisionAb™ is a trademark of Bio-Rad Laboratories
- Acknowledgements
- PrecisionAb™ is a trademark of Bio-Rad Laboratories
- Regulatory
- For research purposes only
Applications of Chk1 antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Western Blotting | 1/1000 | ||
| Western Blotting | 1/1000 |
PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
Copyright © 2018 Bio-Rad Antibodies (formerly AbD Serotec)
Secondary Antibodies Available
| Description | Product Code | Pack Size | Applications | List Price | Quantity |
|---|---|---|---|---|---|
| Goat anti Rabbit IgG (H/L):HRP | STAR208P | 2 ml | WB | ||
| Goat anti Rabbit IgG (H/L):HRP | STAR208P | 2 ml | WB |
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