Chk1 antibody

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Rabbit anti Chk1

Rabbit anti-human CHK1 antibody recognizes serine/threonine-protein kinase CHK1, also known as CHK1 checkpoint homolog, cell cycle checkpoint kinase or checkpoint kinase-1. This antibody has been extensively validated for western blotting using whole cell lysates.

Chk1 Antibody with Control Lysate

Product Type: PrecisionAb™ Polyclonal
Isotype: Polyclonal IgG
Format: Purified

Product Code	Applications	Pack Size
VPA00572	WB	100 µl
VPA00572KT	WB	2 Westerns
With Lysate		400µg

Summary
Secondary Antibodies

Rabbit anti Human Chk1 antibody recognizes Chk1 also known as checkpoint kinase-1, CHK1 checkpoint homolog or cell cycle checkpoint kinase .

The protein encoded by CHEK1 gene belongs to the Ser/Thr protein kinase family. It is required for checkpoint mediated cell cycle arrest in response to DNA damage or the presence of unreplicated DNA. This protein acts to integrate signals from ATM and ATR, two cell cycle proteins involved in DNA damage responses, that also associate with chromatin in meiotic prophase I. Phosphorylation of CDC25A protein phosphatase by this protein is required for cells to delay cell cycle progression in response to double-strand DNA breaks. Several alternatively spliced transcript variants have been found for CHEK1 (provided by RefSeq, Oct 2011).

Rabbit anti Human Chk1 antibody detects a band of 56 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.

Product Details

Target Species

Human

Western Blotting

Anti Chk1 detects a band of approximately 56 kDa in HeLa cell lysates.

Species Cross-Reactivity

Target Species	Cross Reactivity
Mouse
Rat

N.B. Antibody reactivity and working conditions may vary between species.

Product Form

Purified IgG - liquid.

Product Form

Purified IgG - liquid.

Preparation

Rabbit polyclonal antibody purified by affinity chromatography.

Preparation

20μl Rabbit polyclonal antibody purified by affinity chromatography.

Buffer Solution

Phosphate buffered saline.

Buffer Solution

Phosphate buffered saline.

Preservative Stabilisers

0.09% Sodium Azide (NaN₃).

Preservative Stabilisers

0.09% Sodium Azide (NaN₃).

Immunogen

KLH conjugated synthetic peptide corresponding to 258-287 amino acid from human Chk1

Instructions For Use

Please refer to the PrecisionAb western blotting protocol.

Positive Control Lysate

Lysate Composition: 400μg HeLa lysate lyophilized in RIPA buffer.
Lysate Reconstitution: - If using DDT reconstitute the lyophilized lysate with 190μl DI H₂O, add 200μl 2x Laemmli Sample Buffer and 10μl 2M DTT.
- If using BME reconstitute the lyophilized lysate with 180μl DI H₂O, add 200μl 2x Laemmli Sample Buffer and 20μl BME.
Heat at 95°C for 5 minutes. For 10 well mini gels load 25μl. For other gel and comb formats please refer to the PrecisionAb western blotting protocol.

Storage Information

Storage: Store undiluted at -20°C, avoiding repeated freeze thaw cycles.
Storage: Antibody: Store undiluted at -20°C, avoiding repeated freeze thaw cycles.

Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage.
Shelf Life: As supplied, 12 months from date of despatch.
Shelf Life: As supplied, 12 months from date of despatch.

More Information

UniProt: O14757 Related reagents
Entrez Gene: CHEK1 Related reagents
GO Terms: GO:0006260 DNA replication; GO:0000077 DNA damage checkpoint; GO:0000079 regulation of cyclin-dependent protein kinase activity; GO:0005515 protein binding; GO:0000794 condensed nuclear chromosome; GO:0004674 protein serine/threonine kinase activity; GO:0005524 ATP binding; GO:0005654 nucleoplasm; GO:0005815 microtubule organizing center; GO:0006281 DNA repair; GO:0006468 protein phosphorylation; GO:0007131 reciprocal meiotic recombination; GO:0007276 gamete generation; GO:0008285 negative regulation of cell proliferation; GO:0090399 replicative senescence
Acknowledgements: PrecisionAb™ is a trademark of Bio-Rad Laboratories.
Acknowledgements: PrecisionAb™ is a trademark of Bio-Rad Laboratories.
Regulatory: For research purposes only.

Applications of Chk1 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Verified	Min Dilution	Max Dilution
Western Blotting		1/1000
Western Blotting		1/1000

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.

Secondary Antibodies Available

Description	Product Code	Pack Size	Applications	List Price	Quantity
Goat anti Rabbit IgG (H/L):HRP	STAR208P	2 ml	WB
Goat anti Rabbit IgG (H/L):HRP	STAR208P	2 ml	WB

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