Cerk antibody
Rabbit anti Cerk
Rabbit anti human CERK antibody recognizes ceramide kinase, also known as acylsphingosine kinase or lipid kinase 4. This antibody has been extensively validated for western blotting using whole cell lysates.
Cerk Antibody with Control Lysate
Rabbit anti human CERK antibody recognizes ceramide kinase, also known as acylsphingosine kinase or lipid kinase 4. This antibody has been extensively validated for western blotting using whole cell lysates.
- Product Type
- PrecisionAb™ Polyclonal
- Isotype
- Polyclonal IgG
| Product Code | Applications | Datasheet | MSDS | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|---|
| VPA00259 | WB | 100 µl | ||||
| VPA00259KT | WB | 2 Westerns | ||||
| With Lysate | 400µg |
Rabbit anti Human CERK antibody recognizes CERK, also known as ceramide kinase, acylsphingosine kinase, lipid kinase 4 and lipid kinase LK4.
CERK converts ceramide to ceramide 1-phosphate (C1P), a sphingolipid metabolite. Both CERK and C1P have been implicated in various cellular processes, including proliferation, apoptosis, phagocytosis, and inflammation (Kim et al. 2006. PubMed 16488390, OMIM, Mar 2008).
Rabbit anti Human CERK antibody detects a band of 60 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.
CERK converts ceramide to ceramide 1-phosphate (C1P), a sphingolipid metabolite. Both CERK and C1P have been implicated in various cellular processes, including proliferation, apoptosis, phagocytosis, and inflammation (Kim et al. 2006. PubMed 16488390, OMIM, Mar 2008).
Rabbit anti Human CERK antibody detects a band of 60 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.
Product Details
- Target Species
- Human
- Species Cross-Reactivity
-
Target Species Cross Reactivity Mouse - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Ig fraction - liquid
- Product Form
- Ig fraction - liquid
- Preparation
- Rabbit Ig fraction prepared by ammonium sulphate precipitation
- Preparation
- 20μl Rabbit Ig fraction prepared by ammonium sulphate precipitation
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
- Immunogen
- KLH-conjugated synthetic peptide corresponding to aa 487-516 of human CERK
Storage Information
- Storage
- Store undiluted at -20oC, avoiding repeated freeze thaw cycles
- Storage
- Antibody: Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage. - Shelf Life
- As supplied, 12 months from date of despatch
- Shelf Life
- As supplied, 12 months from date of despatch
More Information
- UniProt
- Q8TCT0 Related reagents
- Entrez Gene
- CERK Related reagents
- GO Terms
- GO:0000287 magnesium ion binding
- GO:0000299 integral to membrane of membrane fraction
- GO:0001729 ceramide kinase activity
- GO:0016020 membrane
- GO:0004143 diacylglycerol kinase activity
- GO:0005524 ATP binding
- GO:0005634 nucleus
- GO:0006672 ceramide metabolic process
- GO:0007205 activation of protein kinase C activity by G-protein coupled receptor protein signaling pathway
- Acknowledgements
- PrecisionAb™ is a trademark of Bio-Rad Laboratories
- Acknowledgements
- PrecisionAb™ is a trademark of Bio-Rad Laboratories
- Regulatory
- For research purposes only
Applications of Cerk antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Western Blotting | 1/1000 | ||
| Western Blotting | 1/1000 |
PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
- Western Blotting
- Anti CERK detects a band of approximately 60 kDa in HepG2 cell lysate
- Western Blotting
- Anti CERK detects a band of approximately 60 kDa in HepG2 cell lysate
- Instructions For Use
- Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.
- Instructions For Use
- Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.
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Secondary Antibodies Available
| Description | Product Code | Pack Size | Applications | List Price | Quantity |
|---|---|---|---|---|---|
| Goat anti Rabbit IgG (H/L):HRP | STAR208P | 2 ml | WB | ||
| Goat anti Rabbit IgG (H/L):HRP | STAR208P | 2 ml | WB |
