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Cerk antibody

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Western Blot Validated
Anti Cerk Antibody (PrecisionAb Polyclonal Antibody) gallery image 1
Western blot analysis of whole cell lysates probed with CERK antibody followed by detection with HRP conjugated Goat anti Rabbit (1/10,000, STAR208P) and visualized on the ChemiDoc MP with 10 second exposure. Arrow points to CERK (molecular weight 60 kDa)

Rabbit anti Cerk

Product Type
PrecisionAb Polyclonal
Isotype
Polyclonal IgG
Format
Purified
PrecisionAb Antibodies
Product Code Applications Pack Size List Price Quantity
VPA00259
Datasheet
WB
SDS
100 µl loader

Rabbit anti Human CERK antibody recognizes CERK, also known as ceramide kinase, acylsphingosine kinase, lipid kinase 4 and lipid kinase LK4.

CERK converts ceramide to ceramide 1-phosphate (C1P), a sphingolipid metabolite. Both CERK and C1P have been implicated in various cellular processes, including proliferation, apoptosis, phagocytosis, and inflammation (Kim et al. 2006).

Rabbit anti Human CERK antibody detects a band of 60 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.

Product Details

Target Species
Human
Western Blotting
Anti CERK detects a band of approximately 60 kDa in HepG2 cell lysate
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Ig fraction - liquid
Preparation
Rabbit Ig fraction prepared by ammonium sulphate precipitation
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Immunogen
KLH-conjugated synthetic peptide corresponding to aa 487-516 of human CERK

Storage Information

Storage
Store undiluted at -20oC, avoiding repeated freeze thaw cycles
Guarantee
12 months from date of despatch

More Information

UniProt
Q8TCT0
Entrez Gene
CERK
GO Terms
GO:0000287 magnesium ion binding
GO:0000299 integral to membrane of membrane fraction
GO:0001729 ceramide kinase activity
GO:0016020 membrane
GO:0004143 diacylglycerol kinase activity
GO:0005524 ATP binding
GO:0005634 nucleus
GO:0006672 ceramide metabolic process
GO:0007205 activation of protein kinase C activity by G-protein coupled receptor protein signaling pathway
Acknowledgements
PrecisionAb is a trademark of Bio-Rad Laboratories.
Regulatory
For research purposes only

Applications of Cerk antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000

The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.
Copyright © 2020 Bio-Rad Antibodies (formerly AbD Serotec)

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Goat anti Rabbit IgG (H/L):HRP STAR208P WB 2 ml

A Precise Solution to Reproducible Western Blots - PrecisionAb Antibodies
Request a different product with the Cerk specificity