Cerk antibody

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Anti Cerk Antibody (PrecisionAb Polyclonal Antibody) gallery image 1 — Western blot analysis of whole cell lysates probed with CERK antibody followed by detection with HRP conjugated Goat anti Rabbit (1/10,000, STAR208P) and visualized on the ChemiDoc MP with 10 second exposure. Arrow points to CERK (molecular weight 60 kDa)

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Rabbit anti Cerk

Product Type: PrecisionAb Polyclonal
Isotype: Polyclonal IgG
Format: Purified
Specificity: Cerk

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VPA00259 Datasheet Datasheet SDS SDS	WB	100 µl		Log in	Get Quote
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Rabbit anti Human CERK antibody recognizes CERK, also known as ceramide kinase, acylsphingosine kinase, lipid kinase 4 and lipid kinase LK4.

CERK converts ceramide to ceramide 1-phosphate (C1P), a sphingolipid metabolite. Both CERK and C1P have been implicated in various cellular processes, including proliferation, apoptosis, phagocytosis, and inflammation (Kim et al. 2006).

Rabbit anti Human CERK antibody detects a band of 60 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.

Target Species: Human
Western Blotting: Anti CERK detects a band of approximately 60 kDa in HepG2 cell lysate
Species Cross-Reactivity: Target Species Cross Reactivity

Mouse; N.B. Antibody reactivity and working conditions may vary between species.
Product Form: Ig fraction - liquid
Preparation: Rabbit Ig fraction prepared by ammonium sulphate precipitation
Buffer Solution: Phosphate buffered saline
Preservative Stabilisers: 0.09% Sodium Azide (NaN₃)
Immunogen: KLH-conjugated synthetic peptide corresponding to aa 487-516 of human CERK
Regulatory: For research purposes only
Guarantee: 12 months from date of despatch
Acknowledgements: PrecisionAb is a trademark of Bio-Rad Laboratories.

Target Species	Cross Reactivity
Mouse

Store undiluted at -20^oC, avoiding repeated freeze thaw cycles

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Verified	Min Dilution	Max Dilution
Western Blotting		1/1000

The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.

Description	Product Code	Applications	Pack Size	Your Price
Goat anti Rabbit IgG (H/L):HRP	STAR208P	WB	2 ml	Log in	Get Quote
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Description	Goat anti Rabbit IgG (H/L):HRP

UniProt: Q8TCT0
Entrez Gene: CERK
GO Terms: GO:0000287 magnesium ion binding; GO:0000299 integral to membrane of membrane fraction; GO:0001729 ceramide kinase activity; GO:0016020 membrane; GO:0004143 diacylglycerol kinase activity; GO:0005524 ATP binding; GO:0005634 nucleus; GO:0006672 ceramide metabolic process; GO:0007205 activation of protein kinase C activity by G-protein coupled receptor protein signaling pathway; View More GO Terms