Centromere Protein F antibody

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Rabbit anti Centromere Protein F

Centromere protein F antibody recognizes centromere protein F, also known as CENP-F kinetochore protein, CENPF, hcp-1, or 350/400 kDa (mitosin).

Centromere Protein F Antibody with Control Lysate

Centromere protein F antibody recognizes centromere protein F, also known as CENP-F kinetochore protein, CENPF, hcp-1, or 350/400 kDa (mitosin).

Product Type
PrecisionAb™ Polyclonal
Isotype
Polyclonal IgG
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
VPA00837 WB 100 µl
VPA00837KT WB 2 Westerns
With Lysate 400µg
Rabbit anti Human centromere protein F antibody recognizes centromere protein F, also known as CENP-F kinetochore protein, cell-cycle-dependent 350K nuclear protein, CENPF, hcp-1 and centromere protein F, 350/400 kDa (mitosin).

CENPF encodes a protein that associates with the centromere-kinetochore complex. The protein is a component of the nuclear matrix during the G2 phase of interphase. It localizes to the spindle midzone and the intracellular bridge in late anaphase and telophase, respectively, and is thought to be subsequently degraded. The localization of this protein suggests that it may play a role in chromosome segregation during mitotis. It is thought to form either a homodimer or heterodimer. Autoantibodies against this protein have been found in patients with cancer or graft versus host disease (provided by RefSeq, Jul 2008).

Rabbit anti Human centromere protein F antibody detects a band of 350 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.

Product Details

Target Species
Human
Product Form
Purified IgG - liquid
Product Form
Purified IgG - liquid
Preparation
Rabbit polyclonal antibody purified by affinity chromatography on immunogen
Preparation
20μl Rabbit polyclonal antibody purified by affinity chromatography on immunogen
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide
<50% Glycerol
Preservative Stabilisers
0.09% Sodium Azide
<50% Glycerol
Immunogen
Recombinant protein of human centromere protein F

Storage Information

Storage
Store undiluted at -20°C, avoiding repeated freeze thaw cycles
Storage
Antibody: Store undiluted at -20oC, avoiding repeated freeze thaw cycles.

Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage.
Shelf Life
As supplied, 12 months from date of despatch
Shelf Life
As supplied, 12 months from date of despatch.

More Information

UniProt
P49454 Related reagents
Entrez Gene
CENPF Related reagents
GO Terms
GO:0006260 DNA replication
GO:0000085 G2 phase of mitotic cell cycle
GO:0000236 mitotic prometaphase
GO:0000922 spindle pole
GO:0000940 condensed chromosome outer kinetochore
GO:0003682 chromatin binding
GO:0005829 cytosol
GO:0005635 nuclear envelope
GO:0007094 mitotic cell cycle spindle assembly checkpoint
GO:0007517 muscle organ development
GO:0008022 protein C-terminus binding
GO:0008134 transcription factor binding
GO:0008283 cell proliferation
GO:0042493 response to drug
GO:0010389 regulation of G2/M transition of mitotic cell cycle
GO:0015031 protein transport
GO:0016202 regulation of striated muscle tissue development
GO:0016363 nuclear matrix
GO:0016481 negative regulation of transcription
GO:0030154 cell differentiation
GO:0030496 midbody
GO:0042803 protein homodimerization activity
GO:0045502 dynein binding
GO:0048471 perinuclear region of cytoplasm
GO:0051301 cell division
GO:0051310 metaphase plate congression
GO:0051382 kinetochore assembly
Acknowledgements
PrecisionAb™ is a trademark of Bio-Rad Laboratories
Acknowledgements
PrecisionAb™ is a trademark of Bio-Rad Laboratories.
Regulatory
For research purposes only

Applications of Centromere Protein F antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000
Western Blotting 1/1000
PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
Western Blotting
Anti centromere protein F antibody recognizes a band of approximately 350 kDa in HeLa cell lysates
Western Blotting
Anti centromere protein F antibody recognizes a band of approximately 350 kDa in HeLa cell lysates
Instructions For Use
Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.
Instructions For Use
Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Goat anti Rabbit IgG (H/L):HRP STAR208P 2 ml WB
Goat anti Rabbit IgG (H/L):HRP STAR208P 2 ml WB