Centromere Protein F antibody

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Anti Centromere Protein F Antibody (PrecisionAb Polyclonal Antibody) gallery image 1 — Western blot analysis of whole cell lysates probed with centromere protein F antibody followed by detection with Goat anti Rabbit HRP conjugated (1/10,000, STAR208P) and visualized on the ChemiDoc MP with 15 second exposure. Arrow points to centromere protein F (molecular weight 350 kDa)

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Rabbit anti Centromere Protein F

Product Type: PrecisionAb Polyclonal
Isotype: Polyclonal IgG
Format: Purified
Specificity: Centromere Protein F

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Rabbit anti Human centromere protein F antibody recognizes centromere protein F, also known as CENP-F kinetochore protein, cell-cycle-dependent 350K nuclear protein, CENPF, hcp-1 and centromere protein F, 350/400 kDa (mitosin).

CENPF encodes a protein that associates with the centromere-kinetochore complex. The protein is a component of the nuclear matrix during the G2 phase of interphase. It localizes to the spindle midzone and the intracellular bridge in late anaphase and telophase, respectively, and is thought to be subsequently degraded. The localization of this protein suggests that it may play a role in chromosome segregation during mitotis. It is thought to form either a homodimer or heterodimer. Autoantibodies against this protein have been found in patients with cancer or graft versus host disease (provided by RefSeq, Jul 2008).

Rabbit anti Human centromere protein F antibody detects a band of 350 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.

Target Species: Human
Western Blotting: Anti centromere protein F antibody recognizes a band of approximately 350 kDa in HeLa cell lysates
Product Form: Purified IgG - liquid
Preparation: Rabbit polyclonal antibody purified by affinity chromatography on immunogen
Buffer Solution: Phosphate buffered saline
Preservative Stabilisers: 0.09% Sodium Azide
<50% Glycerol
Immunogen: Recombinant protein of human centromere protein F
Regulatory: For research purposes only
Guarantee: 12 months from date of despatch
Acknowledgements: PrecisionAb is a trademark of Bio-Rad Laboratories

Store undiluted at -20°C, avoiding repeated freeze thaw cycles

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Verified	Min Dilution	Max Dilution
Western Blotting			1/1000

The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.

Description	Product Code	Applications	Pack Size	Your Price
Goat anti Rabbit IgG (H/L):HRP	STAR208P	WB	2 ml	Log in	Get Quote
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Description	Goat anti Rabbit IgG (H/L):HRP

UniProt: P49454
Entrez Gene: CENPF
GO Terms: GO:0006260 DNA replication; GO:0000085 G2 phase of mitotic cell cycle; GO:0000236 mitotic prometaphase; GO:0000922 spindle pole; GO:0000940 condensed chromosome outer kinetochore; GO:0003682 chromatin binding; GO:0005829 cytosol; GO:0005635 nuclear envelope; GO:0007094 mitotic cell cycle spindle assembly checkpoint; View More GO Terms; GO:0007517 muscle organ development; GO:0008022 protein C-terminus binding; GO:0008134 transcription factor binding; GO:0008283 cell proliferation; GO:0042493 response to drug; GO:0010389 regulation of G2/M transition of mitotic cell cycle; GO:0015031 protein transport; GO:0016202 regulation of striated muscle tissue development; GO:0016363 nuclear matrix; GO:0016481 negative regulation of transcription; GO:0030154 cell differentiation; GO:0030496 midbody; GO:0042803 protein homodimerization activity; GO:0045502 dynein binding; GO:0048471 perinuclear region of cytoplasm; GO:0051301 cell division; GO:0051310 metaphase plate congression; GO:0051382 kinetochore assembly