CELF1 antibody
Rabbit anti CELF1
Rabbit anti human CELF1 antibody recognizes CUGBP Elav-like family member 1, also known as CUGBP1, CUG binding protein 1, bruno-like protein 2, deadenylation factor CUG-BP, or nuclear polyadenylated RNA-binding protein, 50-kDa. This antibody has been extensively validated for western blotting.
CELF1 Antibody with Control Lysate
Rabbit anti human CELF1 antibody recognizes CUGBP Elav-like family member 1, also known as CUGBP1, CUG binding protein 1, bruno-like protein 2, deadenylation factor CUG-BP, embryo deadenylation element binding protein or nuclear polyadenylated RNA-binding protein, 50-kDa.
- Product Type
- PrecisionAb™ Polyclonal
- Isotype
- Polyclonal IgG
- Format
- Purified
| Product Code | Applications | Datasheet | MSDS | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|---|
| VPA00386 | WB | 100 µl | ||||
| VPA00386KT | WB | 2 Westerns | ||||
| With Lysate | 400µg |
Rabbit anti Human CELF1 antibody recognizes the CUGBP Elav-like family member 1, also known as CUG binding protein 1, 50 kDa nuclear polyadenylated RNA-binding protein, bruno-like protein 2, embryo deadenylation element binding protein and nuclear polyadenylated RNA-binding protein, 50 kDa.
Members of the CELF/BRUNOL protein family contain two N-terminal RNA recognition motif (RRM) domains, one C-terminal RRM domain, and a divergent segment of 160-230 aa between the second and third RRM domains. Members of this protein family regulate pre-mRNA alternative splicing and may also be involved in mRNA editing, and translation. CELF1 may play a role in myotonic dystrophy type 1 (DM1) via interactions with the dystrophia myotonica-protein kinase (DMPK) gene. Alternative splicing results in multiple transcript variants encoding different isoforms (provided by RefSeq, Jul 2008).
Rabbit anti Human CELF1 antibody detects a band of 52 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.
Members of the CELF/BRUNOL protein family contain two N-terminal RNA recognition motif (RRM) domains, one C-terminal RRM domain, and a divergent segment of 160-230 aa between the second and third RRM domains. Members of this protein family regulate pre-mRNA alternative splicing and may also be involved in mRNA editing, and translation. CELF1 may play a role in myotonic dystrophy type 1 (DM1) via interactions with the dystrophia myotonica-protein kinase (DMPK) gene. Alternative splicing results in multiple transcript variants encoding different isoforms (provided by RefSeq, Jul 2008).
Rabbit anti Human CELF1 antibody detects a band of 52 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.
Product Details
- Target Species
- Human
- Western Blotting
- Anti CELF1 detects a band of approximately 52 kDa in HeLa cell lysate
- Species Cross-Reactivity
-
Target Species Cross Reactivity Mouse - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Product Form
- Purified IgG - liquid
- Preparation
- Rabbit polyclonal antibody purified by affinity chromatography
- Preparation
- 20μl Rabbit polyclonal antibody purified by affinity chromatography
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
- Immunogen
- KLH-conjugated synthetic peptide corresponding to aa 1-30 of human CELF1
- Instructions For Use
- Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.
Positive Control Lysate
- Lysate Composition
- 400μg HeLa lysate lyophilized in RIPA buffer
- Lysate Reconstitution
- - If using DDT reconstitute the lyophilized lysate with 190μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 10μl 2M DTT.
- If using BME reconstitute the lyophilized lysate with 180μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 20μl BME.
Heat at 95°C for 5 minutes. For 10 well mini gels load 25μl. For other gel and comb formats please refer to the PrecisionAb western blotting protocol.
Storage Information
- Storage
- Store undiluted at -20oC, avoiding repeated freeze thaw cycles
- Storage
- Antibody: Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage. - Shelf Life
- As supplied, 12 months from date of despatch
- Shelf Life
- As supplied, 12 months from date of despatch
More Information
- UniProt
- Q92879 Related reagents
- Entrez Gene
- CELF1 Related reagents
- GO Terms
- GO:0000166 nucleotide binding
- GO:0005515 protein binding
- GO:0000900 translation repressor activity, nucleic acid binding
- GO:0003729 mRNA binding
- GO:0030529 ribonucleoprotein complex
- GO:0005634 nucleus
- GO:0005737 cytoplasm
- GO:0006376 mRNA splice site selection
- GO:0007281 germ cell development
- GO:0009790 embryo development
- GO:0016246 RNA interference
- GO:0042835 BRE binding
- GO:0043484 regulation of RNA splicing
- Acknowledgements
- PrecisionAb™ is a trademark of Bio-Rad Laboratories
- Acknowledgements
- PrecisionAb™ is a trademark of Bio-Rad Laboratories
- Regulatory
- For research purposes only
Applications of CELF1 antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Western Blotting | 1/1000 | ||
| Western Blotting | 1/1000 |
PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
Copyright © 2018 Bio-Rad Antibodies (formerly AbD Serotec)
Secondary Antibodies Available
| Description | Product Code | Pack Size | Applications | List Price | Quantity |
|---|---|---|---|---|---|
| Goat anti Rabbit IgG (H/L):HRP | STAR208P | 2 ml | WB | ||
| Goat anti Rabbit IgG (H/L):HRP | STAR208P | 2 ml | WB |
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