Cebpa antibody

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Anti Cebpa Antibody (PrecisionAb Polyclonal Antibody) gallery image 1 — Western blot analysis of whole cell lysates probed with CEBPA antibody followed by detection with HRP conjugated Goat anti Rabbit IgG (1/10,000, STAR208P) and visualized on the ChemiDoc MP with 14 second exposure. Arrow points to CEBPA (molecular weight 42 kDa)

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Rabbit anti Cebpa

Product Type: PrecisionAb Polyclonal
Isotype: Polyclonal IgG
Format: Purified
Specificity: Cebpa

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Rabbit anti Human CEBPA antibody recognizes the CCAAT/enhancer-binding protein alpha, also known as CCAAT/enhancer-binding protein alpha, CEBP, or C/EBP-alpha.

The protein encoded by the CEBPA intronless gene is a bZIP transcription factor which can bind as a homodimer to certain promoters and enhancers. It can also form heterodimers with the related proteins CEBP-beta and CEBP-gamma. The encoded protein has been shown to bind to the promoter and modulate the expression of the gene encoding leptin, a protein that plays an important role in body weight homeostasis. Also, the encoded protein can interact with CDK2 and CDK4, thereby inhibiting these kinases and causing growth arrest in cultured cells (provided by RefSeq, Jul 2008).

Rabbit anti Human CEBPA antibody detects a band of 42 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.

Target Species: Human
Western Blotting: Rabbit anti CEBPA detects a band of approximately 42 kDa in THP-1 cell lysates.
Product Form: Purified IgG - liquid
Preparation: Rabbit polyclonal antibody purified by affinity chromatography.
Buffer Solution: TRIS buffered glycine.
Preservative Stabilisers: 0.01% Thiomersal
<50% Glycerol
Immunogen: Carrier-protein conjugated synthetic peptide encompassing part of the N-terminal region of human CEBPA
Regulatory: For research purposes only.
Guarantee: 12 months from date of despatch.
Acknowledgements: PrecisionAb is a trademark of Bio-Rad Laboratories.

Store undiluted at -20°C, avoiding repeated freeze thaw cycles.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Verified	Min Dilution	Max Dilution
Western Blotting			1/1000

The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.

Description	Product Code	Applications	Pack Size	Your Price
Goat anti Rabbit IgG (H/L):HRP	STAR208P	WB	2 ml	Log in	Get Quote
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Description	Goat anti Rabbit IgG (H/L):HRP

UniProt: P49715
Entrez Gene: CEBPA
GO Terms: GO:0003705 sequence-specific enhancer binding RNA polymerase II transcription factor activity; GO:0006091 generation of precursor metabolites and energy; GO:0008134 transcription factor binding; GO:0010843 promoter binding; GO:0019221 cytokine-mediated signaling pathway; GO:0044419 interspecies interaction between organisms; GO:0045945 positive regulation of transcription from RNA polymerase III promoter