Cebpa antibody

Rabbit anti Cebpa

Product Type
PrecisionAb Polyclonal
Isotype
Polyclonal IgG
Format
Purified
Specificity
Cebpa

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VPA00700
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Rabbit anti Human CEBPA antibody recognizes the CCAAT/enhancer-binding protein alpha, also known as CCAAT/enhancer-binding protein alpha, CEBP, or C/EBP-alpha.

The protein encoded by the CEBPA intronless gene is a bZIP transcription factor which can bind as a homodimer to certain promoters and enhancers. It can also form heterodimers with the related proteins CEBP-beta and CEBP-gamma. The encoded protein has been shown to bind to the promoter and modulate the expression of the gene encoding leptin, a protein that plays an important role in body weight homeostasis. Also, the encoded protein can interact with CDK2 and CDK4, thereby inhibiting these kinases and causing growth arrest in cultured cells (provided by RefSeq, Jul 2008).

Rabbit anti Human CEBPA antibody detects a band of 42 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.
PrecisionAb Antibodies

Target Species
Human
Western Blotting
Rabbit anti CEBPA detects a band of approximately 42 kDa in THP-1 cell lysates.
Product Form
Purified IgG - liquid
Preparation
Rabbit polyclonal antibody purified by affinity chromatography.
Buffer Solution
TRIS buffered glycine.
Preservative Stabilisers
0.01% Thiomersal
<50% Glycerol
Immunogen
Carrier-protein conjugated synthetic peptide encompassing part of the N-terminal region of human CEBPA
Regulatory
For research purposes only.
Guarantee
12 months from date of despatch.
Acknowledgements
PrecisionAb is a trademark of Bio-Rad Laboratories.

Store undiluted at -20°C, avoiding repeated freeze thaw cycles.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000
The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.

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UniProt
P49715
Entrez Gene
CEBPA
GO Terms
GO:0003705 sequence-specific enhancer binding RNA polymerase II transcription factor activity
GO:0006091 generation of precursor metabolites and energy
GO:0008134 transcription factor binding
GO:0010843 promoter binding
GO:0019221 cytokine-mediated signaling pathway
GO:0044419 interspecies interaction between organisms
GO:0045945 positive regulation of transcription from RNA polymerase III promoter
View more products with CEBPA specificity

Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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