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CDKN2A antibody

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Anti Human CDKN2A (C-Terminal) Antibody gallery image 1
Western blotting analysis of HeLa Lysate (35μg protein in RIPA buffer) using Goat anti Human CDKN2A antibody (AHP1060) at 1.0 μgml-1. Primary incubation was 1 hour. Signal was detected by chemiluminescence.

Goat anti Human CDKN2A (C-Terminal)

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Product Code Applications Pack Size List Price Quantity
AHP1060
Datasheet
E WB
SDS
0.1 mg

Goat anti Human CDKN2A antibody detects an epitope within the C-terminal (CT) region of cyclin-dependent kinase inhibitor 2A (CDKN2A). CDKN2A is a ~16 kDa protein belonging to the CDKN2 cyclin dependent kinase inhibitor family. It acts as a negative regulator of cell proliferation by interacting strongly with CDK4 and CDK6, thus inhibiting their ability to interact with cyclin D and to phosphorylate the retinoblastoma protein. CDKN2A is an important tumour suppressor and defects in its production results in tumour formation in a wide range of tissues, including cutaneous malignant melanoma and Li-Fraumeni syndrome.

Product Details

Target Species
Human
Product Form
Purified IgG - liquid
Antiserum Preparation
Antisera to CDKN2A (isoform 1) were raised by repeated immunisations of goat with highly purified antigen. Purified IgG prepared by affinity chromatography.
Buffer Solution
TRIS buffered saline
Preservative Stabilisers
0.02%Sodium Azide (NaN3)
0.5%Bovine Serum Albumin
Immunogen
Peptide with sequence C-HARIDAAEGPSDIPD from C-terminal of CDKN2A.
Approx. Protein Concentrations
IgG concentration 0.5mg/ml

Storage Information

Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted.
Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Guarantee
12 months from date of despatch

More Information

UniProt
Q8N726
P42771
Entrez Gene
CDKN2A
CDKN2A
GO Terms
GO:0000075 cell cycle checkpoint
GO:0000080 G1 phase of mitotic cell cycle
GO:0000082 G1/S transition of mitotic cell cycle
GO:0005515 protein binding
GO:0001953 negative regulation of cell-matrix adhesion
GO:0005829 cytosol
GO:0004861 cyclin-dependent protein kinase inhibitor activity
GO:0005634 nucleus
GO:0006917 induction of apoptosis
GO:0007050 cell cycle arrest
GO:0007265 Ras protein signal transduction
GO:0008285 negative regulation of cell proliferation
GO:0019901 protein kinase binding
GO:0030308 negative regulation of cell growth
GO:0032088 negative regulation of NF-kappaB transcription factor activity
GO:0034393 positive regulation of smooth muscle cell apoptosis
GO:0045736 negative regulation of cyclin-dependent protein kinase activity
GO:0051059 NF-kappaB binding
GO:0090399 replicative senescence
GO:2000111 positive regulation of macrophage apoptosis
GO:0000209 protein polyubiquitination
GO:0002039 p53 binding
GO:0003677 DNA binding
GO:0005654 nucleoplasm
GO:0005730 nucleolus
GO:0006309 DNA fragmentation involved in apoptotic nuclear change
GO:0006350 transcription
GO:0006364 rRNA processing
GO:0006469 negative regulation of protein kinase activity
GO:0006919 activation of caspase activity
GO:0008637 apoptotic mitochondrial changes
GO:0010389 regulation of G2/M transition of mitotic cell cycle
GO:0016563 transcription activator activity
GO:0050821 protein stabilization
GO:0030889 negative regulation of B cell proliferation
GO:0031648 protein destabilization
GO:0033088 negative regulation of immature T cell proliferation in thymus
GO:0043234 protein complex
GO:0043517 positive regulation of DNA damage response, signal transduction by p53 class mediator
GO:0045944 positive regulation of transcription from RNA polymerase II promoter
GO:0046825 regulation of protein export from nucleus
GO:0048103 somatic stem cell division
GO:0051444 negative regulation of ubiquitin-protein ligase activity
GO:0055105 ubiquitin-protein ligase inhibitor activity
GO:0070215 MDM2 binding
GO:0070534 protein K63-linked ubiquitination
GO:0071158 positive regulation of cell cycle arrest
GO:0090398 cellular senescence
Regulatory
For research purposes only

Applications of CDKN2A antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 1/32,000
Western Blotting 0.1 1.0ug/ml
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Western Blotting
AHP1060 detects a band of approximately 16-18kDa in A431 and Hela cell lysates.
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Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Rabbit anti Goat IgG (Fc):FITC STAR122F F 1 mg loader
Rabbit anti Goat IgG (Fc):HRP STAR122P C E WB 1 mg loader

Product Specific References

References for CDKN2A antibody

  1. Ruas, M. & Peters, G. (1998) The p16INK4a/CDKN2A tumor suppressor and its relatives.
    Biochim Biophys Acta. 1378 (2): F115-77.
  2. Piepkorn, M. (2000) Melanoma genetics: an update with focus on the CDKN2A(p16)/ARF tumor suppressors.
    J Am Acad Dermatol. 42 (5 Pt 1): 705-22; quiz 723-6.

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