CDKN2A antibody

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Anti Human CDKN2A (C-Terminal) Antibody gallery image 1 — Western blotting analysis of HeLa Lysate (35μg protein in RIPA buffer) using Goat anti Human CDKN2A antibody (**AHP1060**) at 1.0 μgml^-1. Primary incubation was 1 hour. Signal was detected by chemiluminescence.

Goat anti Human CDKN2A (C-Terminal)

Product Type: Polyclonal Antibody
Isotype: Polyclonal IgG

Product Code	Applications	Pack Size	Your Price	List Price	Quantity
AHP1060 Datasheet	E WB MSDS	0.1 mg	Log in		Get Quote

Goat anti Human CDKN2A antibody detects an epitope within the C-terminal (CT) region of cyclin-dependent kinase inhibitor 2A (CDKN2A). CDKN2A is a ~16 kDa protein belonging to the CDKN2 cyclin dependent kinase inhibitor family. It acts as a negative regulator of cell proliferation by interacting strongly with CDK4 and CDK6, thus inhibiting their ability to interact with cyclin D and to phosphorylate the retinoblastoma protein. CDKN2A is an important tumour suppressor and defects in its production results in tumour formation in a wide range of tissues, including cutaneous malignant melanoma and Li-Fraumeni syndrome.

Product Details

Target Species

Human

Product Form

Purified IgG - liquid

Antiserum Preparation

Antisera to CDKN2A (isoform 1) were raised by repeated immunisations of goat with highly purified antigen. Purified IgG prepared by affinity chromatography.

Buffer Solution

TRIS buffered saline

Preservative Stabilisers

0.02%	Sodium Azide (NaN₃)
0.5%	Bovine Serum Albumin

Immunogen

Peptide with sequence C-HARIDAAEGPSDIPD from C-terminal of CDKN2A.

Approx. Protein Concentrations

IgG concentration 0.5mg/ml

Storage Information

Storage: Store at +4^oC or at -20^oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted.
Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Guarantee: 18 months from date of despatch.

More Information

UniProt: Q8N726; P42771
Entrez Gene: CDKN2A; CDKN2A
GO Terms: GO:0000075 cell cycle checkpoint; GO:0000080 G1 phase of mitotic cell cycle; GO:0000082 G1/S transition of mitotic cell cycle; GO:0005515 protein binding; GO:0001953 negative regulation of cell-matrix adhesion; GO:0005829 cytosol; GO:0004861 cyclin-dependent protein kinase inhibitor activity; GO:0005634 nucleus; GO:0006917 induction of apoptosis; GO:0007050 cell cycle arrest; GO:0007265 Ras protein signal transduction; GO:0008285 negative regulation of cell proliferation; GO:0019901 protein kinase binding; GO:0030308 negative regulation of cell growth; GO:0032088 negative regulation of NF-kappaB transcription factor activity; GO:0034393 positive regulation of smooth muscle cell apoptosis; GO:0045736 negative regulation of cyclin-dependent protein kinase activity; GO:0051059 NF-kappaB binding; GO:0090399 replicative senescence; GO:2000111 positive regulation of macrophage apoptosis; GO:0000209 protein polyubiquitination; GO:0002039 p53 binding; GO:0003677 DNA binding; GO:0005654 nucleoplasm; GO:0005730 nucleolus; GO:0006309 DNA fragmentation involved in apoptotic nuclear change; GO:0006350 transcription; GO:0006364 rRNA processing; GO:0006469 negative regulation of protein kinase activity; GO:0006919 activation of caspase activity; GO:0008637 apoptotic mitochondrial changes; GO:0010389 regulation of G2/M transition of mitotic cell cycle; GO:0016563 transcription activator activity; GO:0050821 protein stabilization; GO:0030889 negative regulation of B cell proliferation; GO:0031648 protein destabilization; GO:0033088 negative regulation of immature T cell proliferation in thymus; GO:0043234 protein complex; GO:0043517 positive regulation of DNA damage response, signal transduction by p53 class mediator; GO:0045944 positive regulation of transcription from RNA polymerase II promoter; GO:0046825 regulation of protein export from nucleus; GO:0048103 somatic stem cell division; GO:0051444 negative regulation of ubiquitin-protein ligase activity; GO:0055105 ubiquitin-protein ligase inhibitor activity; GO:0070215 MDM2 binding; GO:0070534 protein K63-linked ubiquitination; GO:0071158 positive regulation of cell cycle arrest; GO:0090398 cellular senescence
Regulatory: For research purposes only

Applications of CDKN2A antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Verified	Min Dilution	Max Dilution
ELISA			1/32,000
Western Blotting		0.1	1.0ug/ml

Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

Western Blotting: AHP1060 detects a band of approximately 16-18kDa in A431 and Hela cell lysates.

Secondary Antibodies Available

Description	Product Code	Applications	Pack Size	Your Price	List Price	Quantity
Rabbit anti Goat IgG (Fc):FITC	STAR122F	F	1 mg	Log in		Get Quote
Rabbit anti Goat IgG (Fc):HRP	STAR122P	C E WB	1 mg	Log in		Get Quote

Product Specific References

References for CDKN2A antibody

Ruas, M. & Peters, G. (1998) The p16INK4a/CDKN2A tumor suppressor and its relatives.
Biochim Biophys Acta. 1378 (2): F115-77.
Piepkorn, M. (2000) Melanoma genetics: an update with focus on the CDKN2A(p16)/ARF tumor suppressors.
J Am Acad Dermatol. 42 (5 Pt 1): 705-22; quiz 723-6.

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