Cdk9 antibody
No associated image currently available
Rabbit anti Human Cdk9 antibody recognizes a ~43 kDa member of the cyclin-dependent protein kinase (CDK) family, CDK9, previously named PITALRE. CDK9 is located primarily in the nucleus but unlike most cdc2-like kinases it does not appear to be cell cycle-regulated. Rabbit anti Human Cdk9 antibody does not cross react with other cyclin dependent kinases.
Product Details
- Target Species
- Human
- Species Cross-Reactivity
-
Target Species Cross Reactivity Mouse Rat - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Serum - liquid
- Antiserum Preparation
- Antisera to human Cdk9 were raised by repeated immunisations of rabbits with highly purified peptide antigen. Whole serum is supplied delipidated.
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.01% Sodium Azide (NaN3)
- Immunogen
- Multiple synthetic peptides corresponding to the C-terminal and N-terminal domains of human CDK9
Storage Information
- Storage
- This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. - Guarantee
- 12 months from date of despatch
More Information
- UniProt
- P50750
- Entrez Gene
- CDK9
- GO Terms
- GO:0005515 protein binding
- GO:0003677 DNA binding
- GO:0004693 cyclin-dependent protein kinase activity
- GO:0005524 ATP binding
- GO:0006367 transcription initiation from RNA polymerase II promoter
- GO:0006368 transcription elongation from RNA polymerase II promoter
- GO:0006468 protein phosphorylation
- GO:0008023 transcription elongation factor complex
- GO:0008283 cell proliferation
- GO:0008353 RNA polymerase II carboxy-terminal domain kinase activity
- GO:0016032 viral reproduction
- GO:0050434 positive regulation of viral transcription
- Regulatory
- For research purposes only
Applications of Cdk9 antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA | 1/10,000 | 1/50,000 | |
| Immunohistology - Paraffin | 1/200 | 1/1000 | |
| Immunoprecipitation | |||
| Western Blotting | 1/500 | 1/3000 |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Copyright © 2021 Bio-Rad Antibodies (formerly AbD Serotec)
Secondary Antibodies Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| Goat anti Rabbit IgG (Fc):Biotin | STAR121B | E WB | 1 mg |
|
|
| Goat anti Rabbit IgG (Fc):FITC | STAR121F | F | 1 mg |
|
|
| Goat anti Rabbit IgG (Fc):HRP | STAR121P | E WB | 1 mg |
|
|
| Goat anti Rabbit IgG (H/L):HRP | STAR124P | C E WB | 1 mg |
|
|
| Sheep anti Rabbit IgG:FITC | STAR34B | C F | 1 mg |
|
|
| Sheep anti Rabbit IgG:RPE | STAR35A | F | 1 ml |
|
|
| Sheep anti Rabbit IgG:DyLight®488 | STAR36D488GA | F IF WB | 0.1 mg | ||
| Sheep anti Rabbit IgG:DyLight®680 | STAR36D680GA | F IF WB | 0.1 mg | ||
| Sheep anti Rabbit IgG:Dylight®800 | STAR36D800GA | F IF WB | 0.1 mg |
Useful Reagents Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| TidyBlot Western Blot Detection Reagent:HRP | STAR209P | WB* | 0.5 ml |
Product Specific References
References for Cdk9 antibody
-
Graña X et al. (1994) PITALRE, a nuclear CDC2-related protein kinase that phosphorylates the retinoblastoma protein in vitro.
Proc Natl Acad Sci U S A. 91 (9): 3834-8. -
Mayol, X. et al. (1993) Cloning of a new member of the retinoblastoma gene family (pRb2) which binds to the E1A transforming domain.
Oncogene. 8 (9): 2561-6. -
de Falco, G. & Giordano, A. (1998) CDK9 (PITALRE): a multifunctional cdc2-related kinase.
J Cell Physiol. 177 (4): 501-6.
Fluorescent Spectraviewer
Watch the Tool Tutorial Video ▸How to Use the Spectraviewer
Watch the Tool Tutorial Video ▸- Start by selecting the application you are interested in, with the option to select an instrument from the drop down menu or create a customized instrument
- Select the fluorophores or fluorescent proteins you want to include in your panel to check compatibility
- Select the lasers and filters you wish to include
- Select combined or multi-laser view to visualize the spectra
