Rabbit anti Human Cdk9 antibody recognizes a ~43 kDa member of the cyclin-dependent protein kinase (CDK) family, CDK9, previously named PITALRE. CDK9 is located primarily in the nucleus but unlike most cdc2-like kinases it does not appear to be cell cycle-regulated. Rabbit anti Human Cdk9 antibody does not cross react with other cyclin dependent kinases.
- Target Species
- Species Cross-Reactivity
|Target Species||Cross Reactivity|
- N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Serum - liquid
- Antiserum Preparation
- Antisera to human Cdk9 were raised by repeated immunisations of rabbits with highly purified peptide antigen. Whole serum is supplied delipidated.
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.01% Sodium Azide (NaN3)
- Multiple synthetic peptides corresponding to the C-terminal and N-terminal domains of human CDK9
- Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
- 18 months from date of despatch.
- Entrez Gene
- GO Terms
cyclin-dependent protein kinase activity
transcription initiation from RNA polymerase II promoter
transcription elongation from RNA polymerase II promoter
transcription elongation factor complex
RNA polymerase II carboxy-terminal domain kinase activity
positive regulation of viral transcription
- For research purposes only
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Applications of Cdk9 antibody
|Immunohistology - Paraffin
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
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Secondary Antibodies Available
Useful Reagents Available
Product Specific References
References for Cdk9 antibody
Graña X et al. (1994) PITALRE, a nuclear CDC2-related protein kinase that phosphorylates the retinoblastoma protein in vitro.
Proc Natl Acad Sci U S A. 91 (9): 3834-8.
Mayol, X. et al. (1993) Cloning of a new member of the retinoblastoma gene family (pRb2) which binds to the E1A transforming domain.
Oncogene. 8 (9): 2561-6.
de Falco, G. & Giordano, A. (1998) CDK9 (PITALRE): a multifunctional cdc2-related kinase.
J Cell Physiol. 177 (4): 501-6.