Rabbit anti Human Cdk9 antibody recognizes a ~43 kDa member of the cyclin-dependent protein kinase (CDK) family, CDK9, previously named PITALRE. CDK9 is located primarily in the nucleus but unlike most cdc2-like kinases it does not appear to be cell cycle-regulated. Rabbit anti Human Cdk9 antibody does not cross react with other cyclin dependent kinases.
- Target Species
- Species Cross-Reactivity
|Target Species||Cross Reactivity|
- N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Serum - liquid
- Antiserum Preparation
- Antisera to human Cdk9 were raised by repeated immunisations of rabbits with highly purified peptide antigen. Whole serum is supplied delipidated.
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.01% Sodium Azide (NaN3)
- Multiple synthetic peptides corresponding to the C-terminal and N-terminal domains of human CDK9
- This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
- 12 months from date of despatch
- Entrez Gene
- GO Terms
cyclin-dependent protein kinase activity
transcription initiation from RNA polymerase II promoter
transcription elongation from RNA polymerase II promoter
transcription elongation factor complex
RNA polymerase II carboxy-terminal domain kinase activity
positive regulation of viral transcription
- For research purposes only
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Applications of Cdk9 antibody
|Immunohistology - Paraffin
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Copyright © 2021 Bio-Rad Antibodies (formerly AbD Serotec)
Secondary Antibodies Available
Useful Reagents Available
Product Specific References
References for Cdk9 antibody
Graña X et al. (1994) PITALRE, a nuclear CDC2-related protein kinase that phosphorylates the retinoblastoma protein in vitro.
Proc Natl Acad Sci U S A. 91 (9): 3834-8.
Mayol, X. et al. (1993) Cloning of a new member of the retinoblastoma gene family (pRb2) which binds to the E1A transforming domain.
Oncogene. 8 (9): 2561-6.
de Falco, G. & Giordano, A. (1998) CDK9 (PITALRE): a multifunctional cdc2-related kinase.
J Cell Physiol. 177 (4): 501-6.
How to Use the Spectraviewer
Watch the Tool Tutorial Video ▸
Start by selecting the application you are interested in, with the option to select an instrument from the drop down menu or create a customized instrument
Select the fluorophores or fluorescent proteins you want to include in your panel to check compatibility
Select the lasers and filters you wish to include
Select combined or multi-laser view to visualize the spectra