Cdk9 antibody

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Rabbit anti Cdk9

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
AHP315 E IP P WB 0.1 ml
Rabbit anti Human Cdk9 antibody recognizes a ~43 kDa member of the cyclin-dependent protein kinase (CDK) family, CDK9, previously named PITALRE. CDK9 is located primarily in the nucleus but unlike most cdc2-like kinases it does not appear to be cell cycle-regulated. Rabbit anti Human Cdk9 antibody does not cross react with other cyclin dependent kinases.

Product Details

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse
Rat
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Serum - liquid
Antiserum Preparation
Antisera to human Cdk9 were raised by repeated immunisations of rabbits with highly purified peptide antigen. Whole serum is supplied delipidated.
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.01% Sodium Azide (NaN3)
Immunogen
Multiple synthetic peptides corresponding to the C-terminal and N-terminal domains of human CDK9

Storage Information

Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.

More Information

UniProt
P50750 Related reagents
Entrez Gene
CDK9 Related reagents
GO Terms
GO:0005515 protein binding
GO:0003677 DNA binding
GO:0004693 cyclin-dependent protein kinase activity
GO:0005524 ATP binding
GO:0006367 transcription initiation from RNA polymerase II promoter
GO:0006368 transcription elongation from RNA polymerase II promoter
GO:0006468 protein phosphorylation
GO:0008023 transcription elongation factor complex
GO:0008283 cell proliferation
GO:0008353 RNA polymerase II carboxy-terminal domain kinase activity
GO:0016032 viral reproduction
GO:0050434 positive regulation of viral transcription
Regulatory
For research purposes only

Applications of Cdk9 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 1/10,000 1/50,000
Immunohistology - Paraffin 1/200 1/1000
Immunoprecipitation
Western Blotting 1/500 1/3000
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Sheep anti Rabbit IgG:Biotin 2AB02B 1 ml C E P WB
Goat anti Rabbit IgG (Fc):Biotin STAR121B 1 mg E WB
Goat anti Rabbit IgG (Fc):FITC STAR121F 1 mg F
Goat anti Rabbit IgG (Fc):HRP STAR121P 1 mg E WB
Goat anti Rabbit IgG (H/L):HRP STAR124P 1 mg C E WB
Sheep anti Rabbit IgG:FITC STAR34B 1 mg C F
Sheep anti Rabbit IgG:RPE STAR35A 1 ml F
Sheep anti Rabbit IgG:DyLight®488 STAR36D488GA 0.1 mg F IF WB
Sheep anti Rabbit IgG:DyLight®549 STAR36D549GA 0.1 mg F IF
Sheep anti Rabbit IgG:DyLight®649 STAR36D649GA 0.1 mg F IF
Sheep anti Rabbit IgG:DyLight®680 STAR36D680GA 0.1 mg F IF WB
Sheep anti Rabbit IgG:Dylight®800 STAR36D800GA 0.1 mg F IF WB

Useful Reagents Available

Description Product Code Pack Size Applications List Price Quantity
TidyBlot™ Western Blot Detection Reagent:HRP STAR209P 0.5 ml WB*

Product Specific References

References for Cdk9 antibody

  1. Graña X et al. (1994) PITALRE, a nuclear CDC2-related protein kinase that phosphorylates the retinoblastoma protein in vitro.
    Proc Natl Acad Sci U S A. 91 (9): 3834-8.
  2. Mayol, X. et al. (1993) Cloning of a new member of the retinoblastoma gene family (pRb2) which binds to the E1A transforming domain.
    Oncogene. 8 (9): 2561-6.
  3. de Falco, G. & Giordano, A. (1998) CDK9 (PITALRE): a multifunctional cdc2-related kinase.
    J Cell Physiol. 177 (4): 501-6.