Cdk5 antibody

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Rabbit anti Cdk5 (C-Terminal)

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
AHP1270 E IP WB 50 µl
Rabbit anti Cdk5 antibody detects human cyclin dependent kinase 5 (Cdk5), a proline-directed serine/threonine protein kinase that unlike most Cdks does not function in cell cycle control, and is not regulated by cyclins. Instead, it requires the association p35 or p39 for its kinase activity, resulting in a master regulator of neuronal functions, with roles in neuronal survival and migration, neurotransmitter release and axon guidance.

Cdk5 is ubiquitously expressed, but expression and activity is highest in post mitotic neurones, where p35 and p39 are highly expressed. Deregulation of this kinase is neurotoxic and might contribute to the pathogenesis of Alzheimer's disease and other neurodegenerative disorders.

Product Details

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse
Rat
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Serum - liquid
Antiserum Preparation
Antisera to Cdk5 were raised by repeated immunisations of rabbits with highly purified antigen.
Preservative Stabilisers
0.01%Sodium Azide (NaN3)
Immunogen
Peptide corresponding to C-terminus of human Cdk5 conjugated to Keyhole Limpet Hemocyanin (KLH).

Storage Information

Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.

More Information

UniProt
Q00535 Related reagents
Entrez Gene
CDK5 Related reagents
GO Terms
GO:0016020 membrane
GO:0007596 blood coagulation
GO:0005829 cytosol
GO:0004693 cyclin-dependent protein kinase activity
GO:0043125 ErbB-3 class receptor binding
GO:0005176 ErbB-2 class receptor binding
GO:0005524 ATP binding
GO:0009790 embryo development
GO:0008283 cell proliferation
GO:0030027 lamellipodium
GO:0030424 axon
GO:0030425 dendrite
GO:0030426 growth cone
GO:0030549 acetylcholine receptor activator activity
GO:0031594 neuromuscular junction
GO:0043025 neuronal cell body
GO:0043525 positive regulation of neuron apoptosis
GO:0050321 tau-protein kinase activity
GO:0051301 cell division
Regulatory
For research purposes only

Applications of Cdk5 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 1/500 1/2000
Immunoprecipitation
Western Blotting
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Sheep anti Rabbit IgG:Biotin 2AB02B 1 ml C E P WB
Goat anti Rabbit IgG (Fc):Biotin STAR121B 1 mg E WB
Goat anti Rabbit IgG (Fc):FITC STAR121F 1 mg F
Goat anti Rabbit IgG (Fc):HRP STAR121P 1 mg E WB
Goat anti Rabbit IgG (H/L):HRP STAR124P 1 mg C E WB
Sheep anti Rabbit IgG:FITC STAR34B 1 mg C F
Sheep anti Rabbit IgG:RPE STAR35A 1 ml F
Sheep anti Rabbit IgG:DyLight®488 STAR36D488GA 0.1 mg F IF WB
Sheep anti Rabbit IgG:DyLight®549 STAR36D549GA 0.1 mg F IF
Sheep anti Rabbit IgG:DyLight®649 STAR36D649GA 0.1 mg F IF
Sheep anti Rabbit IgG:DyLight®680 STAR36D680GA 0.1 mg F IF WB
Sheep anti Rabbit IgG:Dylight®800 STAR36D800GA 0.1 mg F IF WB

Useful Reagents Available

Description Product Code Pack Size Applications List Price Quantity
TidyBlot™ Western Blot Detection Reagent:HRP STAR209P 0.5 ml WB*

Product Specific References

Further Reading

  1. Cheung, Z.H. et al. (2006) Synaptic roles of Cdk5: implications in higher cognitive functions and neurodegenerative diseases.
    Neuron. 50 (1): 13-8.
  2. Pareek, T.K. & Kulkarni, A.B. (2006) Cdk5: a new player in pain signaling.
    Cell Cycle. 5 (6): 585-8.
  3. Cruz, J.C. & Tsai, L.H. (2004) Cdk5 deregulation in the pathogenesis of Alzheimer's disease.
    Trends Mol Med. 10 (9): 452-8.
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