CDK4 antibody

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Anti CDK4 Antibody (PrecisionAb Polyclonal Antibody) thumbnail image 1

Anti CDK4 Antibody (PrecisionAb Polyclonal Antibody) thumbnail image 2

Anti CDK4 Antibody (PrecisionAb Polyclonal Antibody) thumbnail image 3

Anti CDK4 Antibody (PrecisionAb Polyclonal Antibody) gallery image 1 — Western blot analysis of whole cell lysates probed with CDK4 antibody followed by detection with Goat anti Rabbit HRP conjugated (1/10,000, STAR208P) and visualized on the ChemiDoc MP with 33 second exposure. Arrow points to CDK4 (molecular weight 30 kDa)

Anti CDK4 Antibody (PrecisionAb Polyclonal Antibody) gallery image 2 — Western blot analysis of CDK4 knockout HeLa and wild type HeLa (WT) whole cell lysates probed with (A) Rabbit anti CDK4 antibody (**VPA00749**) followed by detection with HRP conjugated Goat anti Rabbit IgG (1/10,000 STAR208P) and (B) hFAB rhodamine anti-tubulin primary antibody (12004166) and visualized on the ChemiDoc MP with a (A) 475 second and (B) 30 second exposure.

Anti CDK4 Antibody (PrecisionAb Polyclonal Antibody) gallery image 3 — Normalized signal intensity of CDK4 from CRISPR knockout and wild type lysates. Signal intensity was normalized to the tubulin signal

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Rabbit anti CDK4

Product Type: PrecisionAb Polyclonal
Isotype: Polyclonal IgG
Format: Purified
Specificity: CDK4

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Product Code Applications Pack Size List Price Your Price Qty

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VPA00749 Datasheet Datasheet SDS SDS	WB	100 µl		Log in	Get Quote
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Rabbit anti Human CDK4 antibody recognizes cyclin-dependent kinase 4, also known as cell division protein kinase 4.

The protein encoded by CDK4 is a member of the Ser/Thr protein kinase family. This protein is highly similar to the gene products of S. cerevisiae cdc28 and S. pombe cdc2. It is a catalytic subunit of the protein kinase complex that is important for cell cycle G1 phase progression. The activity of this kinase is restricted to the G1-S phase, which is controlled by the regulatory subunits D-type cyclins and CDK inhibitor p16(INK4a). This kinase was shown to be responsible for the phosphorylation of retinoblastoma gene product (Rb). Mutations in CDK4 as well as in its related proteins including D-type cyclins, p16(INK4a) and Rb were all found to be associated with tumorigenesis of a variety of cancers. Multiple polyadenylation sites of CDK4 have been reported (provided by RefSeq, Jul 2008).

Rabbit anti Human CDK4 antibody detects a band of 30 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.

Target Species: Human
Western Blotting: Anti CDK4 antibody recognizes a band of approximately 30 kDa in HEK293 cell lysates.
Product Form: Purified IgG - liquid
Preparation: Rabbit polyclonal antibody purified by affinity chromatography on immunogen.
Buffer Solution: Phosphate buffered saline.
Preservative Stabilisers: 0.09% Sodium Azide (NaN₃)
2% Sucrose
Immunogen: Synthetic peptide encompassing the center region of human CDK4
Approx. Protein Concentrations: IgG concentration 0.5 mg/ml.
Regulatory: For research purposes only.
Guarantee: 12 months from date of despatch.
Acknowledgements: PrecisionAb is a trademark of Bio-Rad Laboratories.

Store undiluted at -20°C, avoiding repeated freeze thaw cycles.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Verified	Min Dilution	Max Dilution
Western Blotting			1/1000

The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.

Description	Product Code	Applications	Pack Size	Your Price
Goat anti Rabbit IgG (H/L):HRP	STAR208P	WB	2 ml	Log in	Get Quote
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Description	Goat anti Rabbit IgG (H/L):HRP

UniProt: P11802
Entrez Gene: CDK4
GO Terms: GO:0000080 G1 phase of mitotic cell cycle; GO:0000082 G1/S transition of mitotic cell cycle; GO:0000084 S phase of mitotic cell cycle; GO:0005515 protein binding; GO:0000307 cyclin-dependent protein kinase holoenzyme complex; GO:0005829 cytosol; GO:0004693 cyclin-dependent protein kinase activity; GO:0005524 ATP binding; GO:0006468 protein phosphorylation; View More GO Terms; GO:0042493 response to drug; GO:0010468 regulation of gene expression; GO:0048146 positive regulation of fibroblast proliferation; GO:0051301 cell division