CDK2 (pThr160) antibody

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Rabbit anti CDK2 (pThr160)

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
AHP2570 P WB 50 µl
Rabbit anti Cdk2 (pThr160) antibody recognizes cyclin-dependent kinase 2 (CDK2) also known as cell division protein kinase 2 and p33 protein kinase when phosphorylated at threonine 160. CDK2 belongs to the serine/threonine protein kinase family and CDC2/CDKX sub family and plays a vital role as part of the cell cycle machinery. Threonine 160 and tyrosine 15 are two major phosphorylation sites. Dephosphorylation of threonine 14 and tryrosine 15 and phosphorylation of threonine 160 are required to activate CDK2 (Gu et al. 1992).

Product Details

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse Expected from Sequence
Rat Expected from Sequence
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Antiserum Preparation
Antiserum to Cdk2 (pThr160) was raised by repeated immunization of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.02% Sodium Azide
50% Glycerol
Immunogen
Phospho specific-peptide corresponding to residues surrounding threonine 160 of human Cdk2

Storage Information

Storage
Store at -20oC
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
12 months from date of despatch

More Information

UniProt
P24941 Related reagents
Entrez Gene
CDK2 Related reagents
GO Terms
GO:0006260 DNA replication
GO:0000082 G1/S transition of mitotic cell cycle
GO:0000084 S phase of mitotic cell cycle
GO:0000085 G2 phase of mitotic cell cycle
GO:0000086 G2/M transition of mitotic cell cycle
GO:0000216 M/G1 transition of mitotic cell cycle
GO:0000307 cyclin-dependent protein kinase holoenzyme complex
GO:0007596 blood coagulation
GO:0005829 cytosol
GO:0004693 cyclin-dependent protein kinase activity
GO:0005524 ATP binding
GO:0006275 regulation of DNA replication
GO:0006977 DNA damage response, signal transduction by p53 class mediator resulting in cell cycle arrest
GO:0007067 mitosis
GO:0007089 traversing start control point of mitotic cell cycle
GO:0007265 Ras protein signal transduction
GO:0008284 positive regulation of cell proliferation
GO:0042802 identical protein binding
GO:0031145 anaphase-promoting complex-dependent proteasomal ubiquitin-dependent protein catabolic process
GO:0051301 cell division
GO:0051439 regulation of ubiquitin-protein ligase activity involved in mitotic cell cycle
Regulatory
For research purposes only

Applications of CDK2 (pThr160) antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Immunohistology - Paraffin 1/50 1/100
Western Blotting 1/500 1/2000
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Western Blotting
Due to the presence of phosphoproteins in milk, the use of milk based blocking reagents is not recommended. 1% BSA in PBS or TBS Tween should be used instead.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Sheep anti Rabbit IgG:Biotin 2AB02B 1 ml C E P WB
Goat anti Rabbit IgG (Fc):Biotin STAR121B 1 mg E WB
Goat anti Rabbit IgG (Fc):FITC STAR121F 1 mg F
Goat anti Rabbit IgG (Fc):HRP STAR121P 1 mg E WB
Goat anti Rabbit IgG (H/L):HRP STAR124P 1 mg C E WB
Sheep anti Rabbit IgG:FITC STAR34B 1 mg C F
Sheep anti Rabbit IgG:RPE STAR35A 1 ml F
Sheep anti Rabbit IgG:DyLight®488 STAR36D488GA 0.1 mg F IF WB
Sheep anti Rabbit IgG:DyLight®549 STAR36D549GA 0.1 mg F IF
Sheep anti Rabbit IgG:DyLight®649 STAR36D649GA 0.1 mg F IF
Sheep anti Rabbit IgG:DyLight®680 STAR36D680GA 0.1 mg F IF WB
Sheep anti Rabbit IgG:Dylight®800 STAR36D800GA 0.1 mg F IF WB

Useful Reagents Available

Description Product Code Pack Size Applications List Price Quantity
TidyBlot™ Western Blot Detection Reagent:HRP STAR209P 0.5 ml WB*

Product Specific References

Further Reading

  1. Gu, Y. et al. (1992) Cell cycle regulation of CDK2 activity by phosphorylation of Thr160 and Tyr15.
    EMBO J. 11 (11): 3995-4005.