Rabbit anti Cdk2 (pThr160) antibody
recognizes cyclin-dependent kinase 2 (CDK2) also known as cell division protein kinase 2 and p33 protein kinase when phosphorylated at threonine 160. CDK2 belongs to the serine/threonine protein kinase family and CDC2/CDKX sub family and plays a vital role as part of the cell cycle machinery. Threonine 160 and tyrosine 15 are two major phosphorylation sites. Dephosphorylation of threonine 14 and tryrosine 15 and phosphorylation of threonine 160 are required to activate CDK2 (Gu et al. 1992
- Target Species
- Species Cross-Reactivity
|Target Species||Cross Reactivity|
||Expected from Sequence
||Expected from Sequence
- N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Antiserum Preparation
- Antiserum to Cdk2 (pThr160) was raised by repeated immunization of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.02% Sodium Azide
- Phospho specific-peptide corresponding to residues surrounding threonine 160 of human Cdk2
- Store at -20oC
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
- 12 months from date of despatch
- Entrez Gene
- GO Terms
G1/S transition of mitotic cell cycle
S phase of mitotic cell cycle
G2 phase of mitotic cell cycle
G2/M transition of mitotic cell cycle
M/G1 transition of mitotic cell cycle
cyclin-dependent protein kinase holoenzyme complex
cyclin-dependent protein kinase activity
regulation of DNA replication
DNA damage response, signal transduction by p53 class mediator resulting in cell cycle arrest
traversing start control point of mitotic cell cycle
Ras protein signal transduction
positive regulation of cell proliferation
identical protein binding
anaphase-promoting complex-dependent proteasomal ubiquitin-dependent protein catabolic process
regulation of ubiquitin-protein ligase activity involved in mitotic cell cycle
- For research purposes only
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Applications of CDK2 (pThr160) antibody
|Immunohistology - Paraffin
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Copyright © 2020 Bio-Rad Antibodies (formerly AbD Serotec)
Secondary Antibodies Available
Useful Reagents Available
Product Specific References
Gu, Y. et al. (1992) Cell cycle regulation of CDK2 activity by phosphorylation of Thr160 and Tyr15.
EMBO J. 11 (11): 3995-4005.