CDC42 antibody

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Rabbit anti CDC42

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
AHP2295 P WB 50 µg
Rabbit anti CDC42 antibody recognizes cell division control protein 42 homolog (CDC42) also known as G25K GTP-binding protein. CDC42 is a small GTPase that plays a role in mediating cell migration, fillopodia formation and cell polarization. Based on sequence homology, this antibody may react with Rac protein family members.

Product Details

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse
Rat Expected from Sequence
Bovine Expected from Sequence
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
30% Glycerol
0.5% Bovine Serum Albumin
0.01% Thiomersal
Immunogen
Synthetic peptides surrounding amino acid 144 of human CDC42
Approx. Protein Concentrations
IgG concentration 0.2 mg/ml

Storage Information

Storage
Store at -20oC
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
12 months from date of despatch

More Information

UniProt
P60953 Related reagents
Entrez Gene
CDC42 Related reagents
GO Terms
GO:0005886 plasma membrane
GO:0005515 protein binding
GO:0003924 GTPase activity
GO:0030036 actin cytoskeleton organization
GO:0007596 blood coagulation
GO:0005829 cytosol
GO:0005525 GTP binding
GO:0007163 establishment or maintenance of cell polarity
GO:0007173 epidermal growth factor receptor signaling pathway
GO:0007264 small GTPase mediated signal transduction
GO:0007411 axon guidance
GO:0030175 filopodium
GO:0030225 macrophage differentiation
GO:0031274 positive regulation of pseudopodium assembly
GO:0031295 T cell costimulation
GO:0031333 negative regulation of protein complex assembly
GO:0031996 thioesterase binding
GO:0042059 negative regulation of epidermal growth factor receptor signaling pathway
GO:0042692 muscle cell differentiation
GO:0051056 regulation of small GTPase mediated signal transduction
GO:0051149 positive regulation of muscle cell differentiation
Regulatory
For research purposes only

Applications of CDC42 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Immunohistology - Paraffin 10 20 ug/ml
Western Blotting 0.5 4.0 ug/ml
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Western Blotting
Rabbit anti CDC42 antibody recognizes a band of approximately 21 kDa in 3T3 and Jurkat cell lysates

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Sheep anti Rabbit IgG:Biotin 2AB02B 1 ml C E P WB
Goat anti Rabbit IgG (Fc):Biotin STAR121B 1 mg E WB
Goat anti Rabbit IgG (Fc):FITC STAR121F 1 mg F
Goat anti Rabbit IgG (Fc):HRP STAR121P 1 mg E WB
Goat anti Rabbit IgG (H/L):HRP STAR124P 1 mg C E WB
Sheep anti Rabbit IgG:FITC STAR34B 1 mg C F
Sheep anti Rabbit IgG:RPE STAR35A 1 ml F
Sheep anti Rabbit IgG:DyLight®488 STAR36D488GA 0.1 mg F IF WB
Sheep anti Rabbit IgG:DyLight®549 STAR36D549GA 0.1 mg F IF
Sheep anti Rabbit IgG:DyLight®649 STAR36D649GA 0.1 mg F IF
Sheep anti Rabbit IgG:DyLight®680 STAR36D680GA 0.1 mg F IF WB
Sheep anti Rabbit IgG:Dylight®800 STAR36D800GA 0.1 mg F IF WB