CDC25A (pSer76) antibody
Rabbit anti Cdc25A (pSer76) antibody recognizes M-phase inducer phosphatase 1 also known as dual specificity phosphatase Cdc25A, when phosphorylated at serine 76.
Cdc25A belongs to the MPI phosphatase family and is involved in cell cycle progression. Phosphorylation of Cdc25A at serine 76 by STK38 contributes to the degradation of Cdc25A and DNA damage-induced checkpoint progression of cell cycle phase G2 to M (Fukasawa et al. 2015).
Cdc25A belongs to the MPI phosphatase family and is involved in cell cycle progression. Phosphorylation of Cdc25A at serine 76 by STK38 contributes to the degradation of Cdc25A and DNA damage-induced checkpoint progression of cell cycle phase G2 to M (Fukasawa et al. 2015).
Product Details
- Target Species
- Human
- Species Cross-Reactivity
-
Target Species Cross Reactivity Mouse Expected from Sequence Rat Expected from Sequence - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Antiserum Preparation
- Antiserum to Cdc25A (pSer76) was raised by repeated immunization of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.02% Sodium Azide
50% Glycerol - Immunogen
- Phospho specific-peptide corresponding to residues surrounding serine 76 of human Cdc25A
Storage Information
- Storage
- Store at -20oC
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Shelf Life
- 12 months from date of despatch
More Information
- UniProt
- P30304 Related reagents
- Entrez Gene
- CDC25A Related reagents
- GO Terms
- GO:0006260 DNA replication
- GO:0000075 cell cycle checkpoint
- GO:0000079 regulation of cyclin-dependent protein kinase activity
- GO:0000082 G1/S transition of mitotic cell cycle
- GO:0000084 S phase of mitotic cell cycle
- GO:0000086 G2/M transition of mitotic cell cycle
- GO:0005515 protein binding
- GO:0005829 cytosol
- GO:0004725 protein tyrosine phosphatase activity
- GO:0005654 nucleoplasm
- GO:0006470 protein dephosphorylation
- GO:0007067 mitosis
- GO:0008283 cell proliferation
- GO:0034644 cellular response to UV
- GO:0051301 cell division
- Regulatory
- For research purposes only
Applications of CDC25A (pSer76) antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Immunohistology - Paraffin | 1/50 | 1/100 | |
| Western Blotting | 1/500 | 1/2000 |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Western Blotting
- Due to the presence of phosphoproteins in milk, the use of milk based blocking reagents is not recommended. 1% BSA in PBS or TBS Tween should be used instead.
Copyright © 2018 Bio-Rad Antibodies (formerly AbD Serotec)
Secondary Antibodies Available
| Description | Product Code | Pack Size | Applications | List Price | Quantity |
|---|---|---|---|---|---|
| Sheep anti Rabbit IgG:Biotin | 2AB02B | 1 ml | C E P WB | ||
| Goat anti Rabbit IgG (Fc):Biotin | STAR121B | 1 mg | E WB | ||
| Goat anti Rabbit IgG (Fc):FITC | STAR121F | 1 mg | F | ||
| Goat anti Rabbit IgG (Fc):HRP | STAR121P | 1 mg | E WB | ||
| Goat anti Rabbit IgG (H/L):HRP | STAR124P | 1 mg | C E WB | ||
| Sheep anti Rabbit IgG:FITC | STAR34B | 1 mg | C F | ||
| Sheep anti Rabbit IgG:RPE | STAR35A | 1 ml | F | ||
| Sheep anti Rabbit IgG:DyLight®488 | STAR36D488GA | 0.1 mg | F IF WB | ||
| Sheep anti Rabbit IgG:DyLight®549 | STAR36D549GA | 0.1 mg | F IF | ||
| Sheep anti Rabbit IgG:DyLight®649 | STAR36D649GA | 0.1 mg | F IF | ||
| Sheep anti Rabbit IgG:DyLight®680 | STAR36D680GA | 0.1 mg | F IF WB | ||
| Sheep anti Rabbit IgG:Dylight®800 | STAR36D800GA | 0.1 mg | F IF WB |
Useful Reagents Available
| Description | Product Code | Pack Size | Applications | List Price | Quantity |
|---|---|---|---|---|---|
| TidyBlot™ Western Blot Detection Reagent:HRP | STAR209P | 0.5 ml | WB* |
Product Specific References
Further Reading
-
Fukasawa, T. et al. (2015) Serine-Threonine Kinase 38 regulates CDC25A stability and the DNA damage-induced G2/M checkpoint.
Cell Signal. 27 (8): 1569-75.
