Rabbit anti Cdc25A (pSer76) antibody
recognizes M-phase inducer phosphatase 1 also known as dual specificity phosphatase Cdc25A, when phosphorylated at serine 76.
Cdc25A belongs to the MPI phosphatase family and is involved in cell cycle progression. Phosphorylation of Cdc25A at serine 76 by STK38 contributes to the degradation of Cdc25A and DNA damage-induced checkpoint progression of cell cycle phase G2 to M (Fukasawa et al. 2015
- Target Species
- Species Cross-Reactivity
|Target Species||Cross Reactivity|
||Expected from Sequence
||Expected from Sequence
- N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Antiserum Preparation
- Antiserum to Cdc25A (pSer76) was raised by repeated immunization of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.02% Sodium Azide
- Phospho specific-peptide corresponding to residues surrounding serine 76 of human Cdc25A
- Store at -20oC
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
- 12 months from date of despatch
- Entrez Gene
- GO Terms
cell cycle checkpoint
regulation of cyclin-dependent protein kinase activity
G1/S transition of mitotic cell cycle
S phase of mitotic cell cycle
G2/M transition of mitotic cell cycle
protein tyrosine phosphatase activity
cellular response to UV
- For research purposes only
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Applications of CDC25A (pSer76) antibody
|Immunohistology - Paraffin
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Copyright © 2019 Bio-Rad Antibodies (formerly AbD Serotec)
Secondary Antibodies Available
Useful Reagents Available
Product Specific References
Fukasawa, T. et al. (2015) Serine-Threonine Kinase 38 regulates CDC25A stability and the DNA damage-induced G2/M checkpoint.
Cell Signal. 27 (8): 1569-75.