CDC25A (pSer76) antibody


Cdc25A belongs to the MPI phosphatase family and is involved in cell cycle progression. Phosphorylation of Cdc25A at serine 76 by STK38 contributes to the degradation of Cdc25A and DNA damage-induced checkpoint progression of cell cycle phase G2 to M (Fukasawa et al. 2015).
Product Details
- Target Species
- Human
- Species Cross-Reactivity
-
Target Species Cross Reactivity Mouse Expected from Sequence Rat Expected from Sequence - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Antiserum Preparation
- Antiserum to Cdc25A (pSer76) was raised by repeated immunization of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.02% Sodium Azide
50% Glycerol - Immunogen
- Phospho specific-peptide corresponding to residues surrounding serine 76 of human Cdc25A
Storage Information
- Storage
- Store at -20oC
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Guarantee
- 12 months from date of despatch
More Information
- UniProt
- P30304
- Entrez Gene
- CDC25A
- GO Terms
- GO:0006260 DNA replication
- GO:0000075 cell cycle checkpoint
- GO:0000079 regulation of cyclin-dependent protein kinase activity
- GO:0000082 G1/S transition of mitotic cell cycle
- GO:0000084 S phase of mitotic cell cycle
- GO:0000086 G2/M transition of mitotic cell cycle
- GO:0005515 protein binding
- GO:0005829 cytosol
- GO:0004725 protein tyrosine phosphatase activity
- GO:0005654 nucleoplasm
- GO:0006470 protein dephosphorylation
- GO:0007067 mitosis
- GO:0008283 cell proliferation
- GO:0034644 cellular response to UV
- GO:0051301 cell division
- Regulatory
- For research purposes only
Applications of CDC25A (pSer76) antibody
Application Name | Verified | Min Dilution | Max Dilution |
---|---|---|---|
Immunohistology - Paraffin | 1/50 | 1/100 | |
Western Blotting | 1/500 | 1/2000 |
Secondary Antibodies Available
Description | Product Code | Applications | Pack Size | List Price | Quantity |
---|---|---|---|---|---|
Goat anti Rabbit IgG (Fc):Biotin | STAR121B | E WB | 1 mg |
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Goat anti Rabbit IgG (Fc):FITC | STAR121F | F | 1 mg |
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Goat anti Rabbit IgG (Fc):HRP | STAR121P | E WB | 1 mg |
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Goat anti Rabbit IgG (H/L):HRP | STAR124P | C E WB | 1 mg |
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Sheep anti Rabbit IgG:FITC | STAR34B | C F | 1 mg |
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|
Sheep anti Rabbit IgG:RPE | STAR35A | F | 1 ml |
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Sheep anti Rabbit IgG:DyLight®488 | STAR36D488GA | F IF WB | 0.1 mg | ||
Sheep anti Rabbit IgG:DyLight®680 | STAR36D680GA | F IF WB | 0.1 mg | ||
Sheep anti Rabbit IgG:Dylight®800 | STAR36D800GA | F IF WB | 0.1 mg |
Useful Reagents Available
Description | Product Code | Applications | Pack Size | List Price | Quantity |
---|---|---|---|---|---|
TidyBlot Western Blot Detection Reagent:HRP | STAR209P | WB* | 0.5 ml |
Product Specific References
Further Reading
-
Fukasawa, T. et al. (2015) Serine-Threonine Kinase 38 regulates CDC25A stability and the DNA damage-induced G2/M checkpoint.
Cell Signal. 27 (8): 1569-75.
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