Cdc2 antibody

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Anti Cdc2 Antibody (PrecisionAb Polyclonal Antibody) gallery image 1 — Western blot analysis of whole cell lysates probed with CDC2 antibody followed by detection with Goat anti Rabbit HRP conjugated (1/10,000, STAR208P) and visualized on the ChemiDoc MP with 2 second exposure. Arrow points to CDC2 (molecular weight 34 kDa).

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Rabbit anti Cdc2

Product Type: PrecisionAb Polyclonal
Isotype: Polyclonal IgG
Format: Purified
Specificity: Cdc2

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VPA00762 Datasheet Datasheet SDS SDS	WB	100 µl		Log in	Get Quote
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Rabbit anti Human CDC2 antibody recognizes CDC2, also known as CDK1, CDC28A, P34CDC2, p34 protein kinase or cyclin-dependent kinase 1.

The protein encoded by this gene is a member of the Ser/Thr protein kinase family. This protein is a catalytic subunit of the highly conserved protein kinase complex known as M-phase promoting factor (MPF), which is essential for G1/S and G2/M phase transitions of eukaryotic cell cycle. Mitotic cyclins stably associate with this protein and function as regulatory subunits. The kinase activity of this protein is controlled by cyclin accumulation and destruction through the cell cycle. The phosphorylation and dephosphorylation of this protein also play important regulatory roles in cell cycle control. Alternatively spliced transcript variants encoding different isoforms have been found for this gene (provided by RefSeq, Mar 2009).

Rabbit anti Human CDC2 antibody detects a band of 34 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.

Target Species: Human
Western Blotting: Anti CDC2 antibody recognizes a band of approximately 34 kDa in Jurkat cell lysates.
Product Form: Purified IgG - liquid
Preparation: Rabbit polyclonal antibody purified by affinity chromatography on immunogen.
Buffer Solution: Phosphate buffered saline.
Preservative Stabilisers: 0.09% Sodium Azide (NaN₃)
2% Sucrose
Immunogen: Synthetic peptide corresponding to a portion of the C terminal region of human CDC2
Regulatory: For research purposes only.
Guarantee: 12 months from date of despatch.
Acknowledgements: PrecisionAb is a trademark of Bio-Rad Laboratories.

Store undiluted at -20°C, avoiding repeated freeze thaw cycles.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Verified	Min Dilution	Max Dilution
Western Blotting			1/1000

The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.

Description	Product Code	Applications	Pack Size	Your Price
Goat anti Rabbit IgG (H/L):HRP	STAR208P	WB	2 ml	Log in	Get Quote
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Description	Goat anti Rabbit IgG (H/L):HRP

UniProt: P06493
Entrez Gene: CDK1
GO Terms: GO:0000083 regulation of transcription involved in G1/S phase of mitotic cell cycle; GO:0000186 activation of MAPKK activity; GO:0000187 activation of MAPK activity; GO:0002755 MyD88-dependent toll-like receptor signaling pathway; GO:0002756 MyD88-independent toll-like receptor signaling pathway; GO:0005829 cytosol; GO:0004693 cyclin-dependent protein kinase activity; GO:0005524 ATP binding; GO:0005654 nucleoplasm; View More GO Terms; GO:0005876 spindle microtubule; GO:0006916 anti-apoptosis; GO:0007067 mitosis; GO:0007173 epidermal growth factor receptor signaling pathway; GO:0007265 Ras protein signal transduction; GO:0007411 axon guidance; GO:0008063 Toll signaling pathway; GO:0008286 insulin receptor signaling pathway; GO:0008353 RNA polymerase II carboxy-terminal domain kinase activity; GO:0030496 midbody; GO:0031145 anaphase-promoting complex-dependent proteasomal ubiquitin-dependent protein catabolic process; GO:0034130 toll-like receptor 1 signaling pathway; GO:0034134 toll-like receptor 2 signaling pathway; GO:0034138 toll-like receptor 3 signaling pathway; GO:0034142 toll-like receptor 4 signaling pathway; GO:0034501 protein localization to kinetochore; GO:0045087 innate immune response; GO:0048011 nerve growth factor receptor signaling pathway; GO:0051301 cell division; GO:0051403 stress-activated MAPK cascade; GO:0051437 positive regulation of ubiquitin-protein ligase activity involved in mitotic cell cycle