Cdc2 antibody

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Rabbit anti Cdc2

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
AHP2407 WB 0.1 mg
Rabbit anti Cdc2 antibody recognizes cell division control protein 2 homolog (Cdc2) also known as cyclin-dependent kinase 1 (CDK1) and p34 protein kinase. Cdc2, a serine/threonine kinase, plays a crucial role in regulation of the eukaryotic cell cycle by phosphorylating proteins involved in DNA replication and other important cell cycle progression processes (Enserink and Kolodner 2010). Binding of cyclins to Cdc2 is essential for its regulation, and the kinase only becomes activated once bound to cyclins (Enserink and Kolodner 2010). Cyclin binding has also been reported to play a role in substrate recruitment (Enserink and Kolodner 2010).

Cdc2 activity is highly regulated through phosphorylation by the serine/threonine protein kinase Wee1 (Harvey et al. 2005). This phosphorylation results in Cdc2 inactivation and consequently, cell cycle arrest at the G2/M transition stage (Harvey et al. 2005). Wee1 phosphorylation is counteracted by the Cdc25 phosphatase, which dephosphorylates Cdc2 thereby enabling entry into mitosis (Harvey et al. 2005).

Product Details

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse
Rat
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography
Antiserum Preparation
Antiserum to Cdc2 was raised by repeated immunisation of rabbits with highly purified antigen
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.1% Sodium Azide (NaN3)
0.2% Gelatin
Immunogen
Peptide mapping to the carboxy terminus of human Cdc2
Approx. Protein Concentrations
IgG concentration 0.2 mg/ml

Storage Information

Storage
Store at +4oC. DO NOT FREEZE.
This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
12 months from date of despatch

More Information

UniProt
P06493 Related reagents
Entrez Gene
CDK1 Related reagents
GO Terms
GO:0000083 regulation of transcription involved in G1/S phase of mitotic cell cycle
GO:0000186 activation of MAPKK activity
GO:0000187 activation of MAPK activity
GO:0002755 MyD88-dependent toll-like receptor signaling pathway
GO:0002756 MyD88-independent toll-like receptor signaling pathway
GO:0005829 cytosol
GO:0004693 cyclin-dependent protein kinase activity
GO:0005524 ATP binding
GO:0005654 nucleoplasm
GO:0005876 spindle microtubule
GO:0006916 anti-apoptosis
GO:0007067 mitosis
GO:0007173 epidermal growth factor receptor signaling pathway
GO:0007265 Ras protein signal transduction
GO:0007411 axon guidance
GO:0008063 Toll signaling pathway
GO:0008286 insulin receptor signaling pathway
GO:0008353 RNA polymerase II carboxy-terminal domain kinase activity
GO:0030496 midbody
GO:0031145 anaphase-promoting complex-dependent proteasomal ubiquitin-dependent protein catabolic process
GO:0034130 toll-like receptor 1 signaling pathway
GO:0034134 toll-like receptor 2 signaling pathway
GO:0034138 toll-like receptor 3 signaling pathway
GO:0034142 toll-like receptor 4 signaling pathway
GO:0034501 protein localization to kinetochore
GO:0045087 innate immune response
GO:0048011 nerve growth factor receptor signaling pathway
GO:0051301 cell division
GO:0051403 stress-activated MAPK cascade
GO:0051437 positive regulation of ubiquitin-protein ligase activity involved in mitotic cell cycle
Regulatory
For research purposes only

Applications of Cdc2 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/400
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Western Blotting
Rabbit anti Cdc2 recognizes a band of approximately 34 kDa when run under reducing conditions

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Sheep anti Rabbit IgG:Biotin 2AB02B 1 ml C E P WB
Goat anti Rabbit IgG (Fc):Biotin STAR121B 1 mg E WB
Goat anti Rabbit IgG (Fc):FITC STAR121F 1 mg F
Goat anti Rabbit IgG (Fc):HRP STAR121P 1 mg E WB
Goat anti Rabbit IgG (H/L):HRP STAR124P 1 mg C E WB
Sheep anti Rabbit IgG:FITC STAR34B 1 mg C F
Sheep anti Rabbit IgG:RPE STAR35A 1 ml F
Sheep anti Rabbit IgG:DyLight®488 STAR36D488GA 0.1 mg F IF WB
Sheep anti Rabbit IgG:DyLight®549 STAR36D549GA 0.1 mg F IF
Sheep anti Rabbit IgG:DyLight®649 STAR36D649GA 0.1 mg F IF
Sheep anti Rabbit IgG:DyLight®680 STAR36D680GA 0.1 mg F IF WB
Sheep anti Rabbit IgG:Dylight®800 STAR36D800GA 0.1 mg F IF WB

Useful Reagents Available

Description Product Code Pack Size Applications List Price Quantity
TidyBlot Western Blot Detection Reagent:HRP STAR209P 0.5 ml WB*
Eukaryotic cell cycle diagram

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Understanding the Eukaryotic Cell Cycle - A Biological and Experimental Overview

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