CD98 Light Chain antibody

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Rabbit anti Human CD98 Light Chain (N-Terminal)

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
AHP1139 P* WB 50 µg
Rabbit anti Human CD98 light chain antibody detects an epitope within the N-terminal (NT) region of the CD98 light chain (CD98LC), also known as the 4F2 light chain, or LAT-1. CD98LC is a ~40 kDa multi-pass membrane glycoprotein which forms a heterodimer with the CD98 heavy chain to allow transport of large branched chain, neutral amino acids into the cell. This transport system leads to the uptake of seven amino acids, including isoleucine, phenylalanine, and methionine.

The expression levels of CD98LC are upregulated in a wide spectrum of primary human cancers. Changes in amino acid transporter expression are thought to be an early change in oncogenesis, as they impart growth and survival advantages.

Product Details

Target Species
Human
Product Form
Purified IgG - liquid
Antiserum Preparation
Antisera to CD98 light chain were raised by repeated immunisations of rabbit with highly purified antigen. Purified IgG prepared by affinity chromatography.
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.1%Sodium Azide (NaN3)
Immunogen
Synthetic peptide corresponding to the N-terminal region of CD98 light chain
Approx. Protein Concentrations
IgG concentration 1.0mg/ml

Storage Information

Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.

More Information

UniProt
Q01650 Related reagents
Entrez Gene
SLC7A5 Related reagents
GO Terms
GO:0007596 blood coagulation
GO:0016021 integral to membrane
GO:0005829 cytosol
GO:0042605 peptide antigen binding
GO:0006520 cellular amino acid metabolic process
GO:0006811 ion transport
GO:0007399 nervous system development
GO:0015175 neutral amino acid transmembrane transporter activity
GO:0016324 apical plasma membrane
GO:0030154 cell differentiation
GO:0050900 leukocyte migration
Regulatory
For research purposes only

Applications of CD98 Light Chain antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Immunohistology - Paraffin 1 15 25ug/ml
Western Blotting 1 10ug/ml
  1. 1This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.Sodium citrate buffer pH 6.0 is recommended for this purpose.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Histology Positive Control Tissue
Placenta
Western Blotting
AHP1139 detects a band of approximately 50-55kDa in human liver cell lysates.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Sheep anti Rabbit IgG:Biotin 2AB02B 1 ml C E P WB
Goat anti Rabbit IgG (Fc):Biotin STAR121B 1 mg E WB
Goat anti Rabbit IgG (Fc):FITC STAR121F 1 mg F
Goat anti Rabbit IgG (Fc):HRP STAR121P 1 mg E WB
Goat anti Rabbit IgG (H/L):HRP STAR124P 1 mg C E WB
Sheep anti Rabbit IgG:FITC STAR34B 1 mg C F
Sheep anti Rabbit IgG:RPE STAR35A 1 ml F
Sheep anti Rabbit IgG:DyLight®488 STAR36D488GA 0.1 mg F IF WB
Sheep anti Rabbit IgG:DyLight®549 STAR36D549GA 0.1 mg F IF
Sheep anti Rabbit IgG:DyLight®649 STAR36D649GA 0.1 mg F IF
Sheep anti Rabbit IgG:DyLight®680 STAR36D680GA 0.1 mg F IF WB
Sheep anti Rabbit IgG:Dylight®800 STAR36D800GA 0.1 mg F IF WB

Useful Reagents Available

Description Product Code Pack Size Applications List Price Quantity
Antigen Retrieval Buffer, pH8.0 BUF025A 500 ml P
Antigen Retrieval Buffer, pH8.0 BUF025C 50 ml P
Histar Detection System STAR3000A 50 Tests C P
Histar Detection System STAR3000B 150 Tests C P
Histar Detection System STAR3000C 500 Tests C P

Product Specific References

References for CD98 Light Chain antibody

  1. Padbury, J.F. et al. (2004) Transcriptional regulation of the LAT-1/CD98 light chain.
    Biochem Biophys Res Commun. 318 (2): 529-34.
  2. Fuchs, B.C. & Bode, B.P. (2005) Amino acid transporters ASCT2 and LAT1 in cancer: partners in crime?
    Semin Cancer Biol. 15 (4): 254-66.