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CD82 antibody

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Anti Human CD82 (C-Terminal) Antibody thumbnail image 1 Anti Human CD82 (C-Terminal) Antibody thumbnail image 2 Anti Human CD82 (C-Terminal) Antibody thumbnail image 3
Anti Human CD82 (C-Terminal) Antibody gallery image 1
Western blot analysis of whole cell lysate from A549 human lung adenocarcinoma cells probed with Rabbit anti Human CD82 (AHP1709) at 0.5(A) and 1(B) ug/ml
Anti Human CD82 (C-Terminal) Antibody gallery image 2
Immunohistological staining of formalin fixed, paraffin embedded human colon tissue sections with Rabbit anti Human CD82 (AHP1709).
Anti Human CD82 (C-Terminal) Antibody gallery image 3
Published customer image:
Rabbit anti Human CD82 antibody (AHP1709) used for the detection of CD82 clusters on a B cell membrane by immunofluorescence.
Image caption:
CD53 does not overlap with CD82. A: B cell membrane sheets double stained for CD82(Rab) (red) and CD82(Mo) (green) were imaged by STED microscopy. Scale bars: 5 μm in whole sheets and 1 μm in zoomed images. Graph: intensity profiles of CD82(Rab) (red curves) and CD82(Mo) (filled green) as depicted in the corresponding images. B: B cells membrane sheets double stained for CD82(Rab) (red) and CD53(Mo) (green) were imaged by STED microscopy. Scale bars: 5 μm in whole sheets and 0.5 μm in zoomed images. Graphs: intensity profiles of CD82 (red line) and CD53 (filled green) as depicted in the corresponding images.

From: Zuidscherwoude, M. et al.
The tetraspanin web revisited by super-resolution microscopy.
Sci. Rep. 5, 12201; doi: 10.1038/srep12201 (2015).
This is from an open access article distributed under the terms of the Creative Commons Attribution License.

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Rabbit anti Human CD82 (C-Terminal)

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Specificity
CD82
Region
(C-TERMINAL)

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Product Code Applications Pack Size List Price Your Price Qty
AHP1709
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
P * WB 0.1 mg loader
List Price Your Price
loader

Rabbit anti Human CD82 antibody recognises the carboxy-terminal region of human CD82, also known as KAI1, SAR2 and TSPAN27. CD82 is a ~30 kDa multi-pass transmembrane glycoprotein belonging to the tetraspanin (TM4SF) family.

CD82 acts as a cancer metastasis suppressor protein (Feng et al.2015), while ubiquitously expressed at moderate to high levels in many normal tissues, its expression is down regulated during tumour progression. Loss of CD82 and p53 is correlated with poor prognosis for patients with prostate and other forms of cancer (Liu et al. 2006).

CD82 interacts with CD234 (DARC). Tumour cells dislodged from the primary tumour and expressing CD82 interact with CD234 expressed on vascular cells, transmitting a senescent signal to the tumour cells (Tsai et al 2011). Tumor cells that have lost CD82 expression can proliferate and potentially give rise to metastases (Miranti 2009).

Two isoforms of CD82 may be generated by alternative splicing. The c-terminal region is not affected and thus Rabbit anti Human CD82 antibody is expected to recognize both isoforms of human CD82 (UniProt: P27701).

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse
Rat
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Antiserum Preparation
Antisera to Human CD82 were raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG prepared from whole serum by affinity chromatography.
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.02% Sodium Azide (NaN3)
Immunogen
A peptide corresponding to a 15 amino acid sequence close to the carboxy terminus of Human CD82.
Approx. Protein Concentrations
IgG concentration 1.0mg/ml
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Immunohistology - Paraffin 1 2.5ug/ml
Western Blotting 0.5 1.0ug/ml
  1. 1This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.Sodium citrate buffer pH 6.0 is recommended for this purpose.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Histology Positive Control Tissue
Human colon tissue
Western Blotting
AHP1709 detects a band of approximately 33kDa in A549 cell lysate

Description Product Code Applications Pack Size List Price Your Price Quantity
Antigen Retrieval Buffer, pH8.0 BUF025A P 500 ml
List Price Your Price
Description Antigen Retrieval Buffer, pH8.0
TidyBlot Western Blot Detection Reagent:HRP STAR209P WB* 0.5 ml
List Price Your Price
Description TidyBlot Western Blot Detection Reagent:HRP

References for CD82 antibody

  1. Zuidscherwoude, M. et al. (2015) The tetraspanin web revisited by super-resolution microscopy.
    Sci Rep. 5: 12201.

Further Reading

  1. Kauffman, E.C. et al. (2003) Metastasis suppression: the evolving role of metastasis suppressor genes for regulating cancer cell growth at the secondary site.
    J Urol. 169 (3): 1122-33.
  2. Dong, J.T. et al. (1995) KAI1, a metastasis suppressor gene for prostate cancer on human chromosome 11p11.2.
    Science. 268 (5212): 884-6.
  3. Gil, M.L. et al. (1992) A member of the tetra spans transmembrane protein superfamily is recognized by a monoclonal antibody raised against an HLA class I-deficient, lymphokine-activated killer-susceptible, B lymphocyte line. Cloning and preliminary functional studies.
    J Immunol. 148 (9): 2826-33.
  4. Gaugitsch, H.W. et al. (1991) A new superfamily of lymphoid and melanoma cell proteins with extensive homology to Schistosoma mansoni antigen Sm23.
    Eur J Immunol. 21 (2): 377-83.

Synonyms
KAI1
RRID
AB_2076397
UniProt
P27701
Entrez Gene
CD82
GO Terms
GO:0005515 protein binding
GO:0005887 integral to plasma membrane

AHP1709

148676

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