CD82 antibody

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Anti Human CD82 (C-Terminal) Antibody thumbnail image 1

Anti Human CD82 (C-Terminal) Antibody thumbnail image 2

Anti Human CD82 (C-Terminal) Antibody thumbnail image 3

Anti Human CD82 (C-Terminal) Antibody gallery image 1 — Western blot analysis of whole cell lysate from A549 human lung adenocarcinoma cells probed with Rabbit anti Human CD82 (**AHP1709**) at 0.5(A) and 1(B) ug/ml

Anti Human CD82 (C-Terminal) Antibody gallery image 2 — Immunohistological staining of human colon with Rabbit anti Human CD82 (**AHP1709**)

Anti Human CD82 (C-Terminal) Antibody gallery image 3 — **Published customer image:**
Rabbit anti Human CD82 antibody used for the detection of CD82 clusters on a B cell membrane by immunofluorescence.
**Image caption:**
CD53 does not overlap with CD82. A: B cell membrane sheets double stained for CD82(Rab) (red) and CD82(Mo) (green) were imaged by STED microscopy. Scale bars: 5 μm in whole sheets and 1 μm in zoomed images. Graph: intensity profiles of CD82(Rab) (red curves) and CD82(Mo) (filled green) as depicted in the corresponding images. B: B cells membrane sheets double stained for CD82(Rab) (red) and CD53(Mo) (green) were imaged by STED microscopy. Scale bars: 5 μm in whole sheets and 0.5 μm in zoomed images. Graphs: intensity profiles of CD82 (red line) and CD53 (filled green) as depicted in the corresponding images.

From: Zuidscherwoude, M. et al. The tetraspanin web revisited by super-resolution microscopy.
Sci. Rep. 5, 12201; doi: 10.1038/srep12201 (2015).

Rabbit anti Human CD82 (C-Terminal)

Product Type: Polyclonal Antibody
Isotype: Polyclonal IgG

Product Code	Applications	Datasheet	MSDS	Pack Size	List Price	Quantity
AHP1709	P* WB			0.1 mg

Rabbit anti Human CD82 antibody recognises the carboxy-terminal region of human CD82, also known as KAI1, SAR2 and TSPAN27. CD82 is a ~30 kDa multi-pass transmembrane glycoprotein belonging to the tetraspanin (TM4SF) family.

CD82 acts as a cancer metastasis suppressor protein (Feng et al.2015), while ubiquitously expressed at moderate to high levels in many normal tissues, its expression is down regulated during tumour progression. Loss of CD82 and p53 is correlated with poor prognosis for patients with prostate and other forms of cancer (Liu et al. 2006).

CD82 interacts with CD234 (DARC). Tumour cells dislodged from the primary tumour and expressing CD82 interact with CD234 expressed on vascular cells, transmitting a senescent signal to the tumour cells (Tsai et al 2011). Tumor cells that have lost CD82 expression can proliferate and potentially give rise to metastases (Miranti 2009).

Two isoforms of CD82 may be generated by alternative splicing. The c-terminal region is not affected and thus Rabbit anti Human CD82 antibody is expected to recognize both isoforms of human CD82 (UniProt: P27701).

Product Details

Target Species

Human

Species Cross-Reactivity

Target Species	Cross Reactivity
Mouse
Rat

N.B. Antibody reactivity and working conditions may vary between species.

Product Form

Purified IgG - liquid

Antiserum Preparation

Antisera to Human CD82 were raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG prepared from whole serum by affinity chromatography.

Buffer Solution

Phosphate buffered saline

Preservative Stabilisers

0.02% Sodium Azide (NaN₃)

Immunogen

A peptide corresponding to a 15 amino acid sequence close to the carboxy terminus of Human CD82.

Approx. Protein Concentrations

IgG concentration 1.0mg/ml

Storage Information

Storage: Store at +4^oC or at -20^oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life: 18 months from date of despatch.

More Information

UniProt: P27701 Related reagents
Entrez Gene: CD82 Related reagents
GO Terms: GO:0005515 protein binding; GO:0005887 integral to plasma membrane
Regulatory: For research purposes only

Applications of CD82 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Verified	Min Dilution	Max Dilution
Immunohistology - Paraffin ¹			2.5ug/ml
Western Blotting		0.5	1.0ug/ml

¹This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.Sodium citrate buffer pH 6.0 is recommended for this purpose.

Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

Histology Positive Control Tissue: Human colon tissue
Western Blotting: AHP1709 detects a band of approximately 33kDa in A549 cell lysate

Useful Reagents Available

Description	Product Code	Pack Size	Applications
Antigen Retrieval Buffer, pH8.0	BUF025A	500 ml	P
Antigen Retrieval Buffer, pH8.0	BUF025C	50 ml	P
TidyBlot™ Western Blot Detection Reagent:HRP	STAR209P	0.5 ml	WB*
Histar Detection System	STAR3000A	50 Tests	C P

Product Specific References

References for CD82 antibody

Zuidscherwoude, M. et al. (2015) The tetraspanin web revisited by super-resolution microscopy.
Sci Rep. 5: 12201.