Rabbit anti CD54 / ICAM-1 antibody
recognizes the CD54 antigen also known as intercellular adhesion molecule 1 (ICAM-1). CD54 is a 532 amino acid single pass type I membrane glycoprotein containing five Ig-like C2-type domains
. CD54 is widely expressed and acts as a ligand for the leukocyte integrin LFA-1 (CD11a/CD18) and Mac-1 integrins, it is the major receptor for human rhinovirus (Staunton et al. 1989
CD54 is highly expressed on activated endothelial cells (Favoloro 1993
) and plays a role in transendothelial cell migration to sites of acute inflammation, functioning primarily as an adhesion molecule between endothelial cells and leucocytes, following injury or stress (Manes and Pober 2011
). Levels of soluble CD54 in serum (Matalliotakis et al. 2001
) or urine (Kim et al. 2006
) can be potentially used as an indication of infection, inflammation and certain tumors, and as a target for immunosuppression in transplantation.
- Target Species
- Species Cross-Reactivity
|Target Species||Cross Reactivity|
- N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 30% Glycerol
0.5% Bovine Serum Albumin
- Synthetic peptide surrounding amino acid 359 of human CD54 / ICAM-1
- Approx. Protein Concentrations
- IgG concentration 0.5 mg/ml
- Store at -20oC
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
- 12 months from date of despatch
- Entrez Gene
- GO Terms
heterophilic cell-cell adhesion
regulation of leukocyte mediated cytotoxicity
T cell activation via T cell receptor contact with antigen bound to MHC molecule on antigen presenting cell
positive regulation of cellular extravasation
transmembrane receptor activity
integral to plasma membrane
leukocyte cell-cell adhesion
membrane to membrane docking
virion attachment, binding of host cell surface receptor
regulation of immune response
adhesion to symbiont
interferon-gamma-mediated signaling pathway
- For research purposes only
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Applications of CD54 / ICAM-1 antibody
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Western Blotting
- Rabbit anti CD54 / ICAM-1 antibody recognizes a band of approximately 89-92 kDa in rat kidney tissue lysate, 3T3 cell lysate and Jurkat cell lysate
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Secondary Antibodies Available