CD54 antibody

Rabbit anti CD54

Product Type
PrecisionAb Polyclonal
Isotype
Polyclonal IgG
Format
Purified
Specificity
CD54

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VPA00014
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Rabbit anti Human CD54 antibody recognizes human CD54, otherwise known as intercellular adhesion molecule 1 or ICAM-1. CD54 is a 532 amino acid single pass type I membrane glycoprotein containing five Ig-like C2-type domains. CD54 is widely expressed and acts as a ligand for the leukocyte integrin LFA-1 (CD11a/CD18) and Mac-1 integrins, it is the major receptor for human rhinovirus (Staunton et al. 1989).

CD54 is highly expressed on activated endothelial cells (Favoloro 1993) and plays a role in transendothelial cell migration to sites of acute inflammation, functioning primarily as an adhesion molecule between endothelial cells and leucocytes, following injury or stress (Manes and Pober 2011). Levels of soluble CD54 in serum (Matalliotakis et al. 2001) or urine (Kim et al. 2006) can be potentially used as an indication of infection, inflammation and certain tumors, and as a target for immunosuppression in transplantation.

Rabbit anti Human CD54 antibody recognizes human CD54 as a single band of ~92 kDa in Raji cell line lysates by western blotting under reducing conditions.
PrecisionAb Antibodies

Target Species
Human
Western Blotting
Anti CD54 antibody detects a band of approximately 92 kDa in Raji cell lysates.
Product Form
Purified IgG - liquid
Preparation
Rabbit polyclonal antibody purified by affinity chromatography.
Buffer Solution
Phosphate buffered saline.
Preservative Stabilisers
0.09% Sodium Azide (NaN3).
Immunogen
Recombinant human CD54
Approx. Protein Concentrations
0.5 mg/ml
Regulatory
For research purposes only.
Guarantee
12 months from date of despatch.
Acknowledgements
PrecisionAb is a trademark of Bio-Rad Laboratories.

Store undiluted at -20°C, avoiding repeated freeze thaw cycles.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA
Immunohistology - Paraffin
Western Blotting 1/1000
The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.

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References for CD54 antibody

  1. Nadlonek, N.A. et al. (2012) Radiation induces osteogenesis in human aortic valve interstitial cells.
    J Thorac Cardiovasc Surg. 144 (6): 1466-70.
  2. Watson, R.L. et al. (2015) Endothelial CD99 signals through soluble adenylyl cyclase and PKA to regulate leukocyte transendothelial migration.
    J Exp Med. 212 (7): 1021-41.

Synonyms
ICAM-1
UniProt
P05362
Entrez Gene
ICAM1
GO Terms
GO:0007157 heterophilic cell-cell adhesion
GO:0001910 regulation of leukocyte mediated cytotoxicity
GO:0002291 T cell activation via T cell receptor contact with antigen bound to MHC molecule on antigen presenting cell
GO:0002693 positive regulation of cellular extravasation
GO:0004888 transmembrane receptor activity
GO:0005887 integral to plasma membrane
GO:0005178 integrin binding
GO:0005615 extracellular space
GO:0007159 leukocyte cell-cell adhesion
GO:0022614 membrane to membrane docking
GO:0046813 virion attachment, binding of host cell surface receptor
GO:0050776 regulation of immune response
GO:0050900 leukocyte migration
GO:0051856 adhesion to symbiont
GO:0060333 interferon-gamma-mediated signaling pathway
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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