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CD54 antibody

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Anti Human CD54 Antibody thumbnail image 1 Anti Human CD54 Antibody thumbnail image 2
Anti Human CD54 Antibody gallery image 1
Paraffin embedded, formalin fixed human breast invasive ductal adenocarcinoma stained with Rabbit anti Human CD54 (AHP2183) following enzyme mediated antigen retrieval using proteinase K.
Anti Human CD54 Antibody gallery image 2
Paraffin embedded, formalin fixed human breast invasive ductal adenocarcinoma stained with Rabbit anti human CD54 (AHP2183) following enzyme mediated antigen retrieval using proteinase K.

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Rabbit anti Human CD54:Biotin

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Specificity
CD54

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Product Code Applications Pack Size List Price Your Price Qty
AHP2183B
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Rabbit anti Human CD54 antibody recognizes human CD54, otherwise known as Intercellular adhesion molecule 1 or ICAM-1. CD54 is a 532 amino acid single pass type I membrane glycoprotein containing five Ig-like C2-type domains. CD54 is widely expressed and acts as a ligand for the leukocyte integrin LFA-1 (CD11a/CD18) and Mac-1 integrins, it is the major receptor for human rhinovirus(Staunton et al. 1989).

CD54 is highly expressed on activated endothelial cells (Favoloro 1993) and plays a role in transendothelial cell migration to sites of acute inflammation, functioning primarily as an adhesion molecule between endothelial cells and leucocytes, following injury or stress (Manes and Pober 2011). Levels of soluble CD54 in serum (Matalliotakis et al. 2001) or urine (Kim et al. 2006), can be potentially used as an indication of infection, inflammation and certain tumour, and as a target for immunosuppression in transplantation.

Target Species
Human
Product Form
Purified IgG conjugated to Biotin - lyophilized
Reconstitution
Reconstitute with 0.5ml sterile PBS containing 0.1% bovine serum albumin
Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution. For long term storage the addition of 0.09% sodium azide is recommended.
Antiserum Preparation
Antiserum to human CD54 was raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG was prepared by affinity chromatography.
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
None present
Carrier Free
Yes
Approx. Protein Concentrations
IgG concentration 0.1mg/ml after reconstitution
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

Prior to reconstitution store at -20°C.
After reconstitution store at -20°C.

This product should be stored undiluted. Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 0.25 1.0ug/ml
Western Blotting 0.1 0.2ug/ml
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
ELISA
This biotinylated human CD54 antibody may be used in a direct ELISA or as the detection reagent in a sandwich ELISA with our purified human CD54 antibody (AHP2183) as the capture reagent and recombinant human CD54 (PHP250) as the standard.

References for CD54 antibody

  1. Nadlonek, N.A. et al. (2012) Radiation induces osteogenesis in human aortic valve interstitial cells.
    J Thorac Cardiovasc Surg. 144 (6): 1466-70.
  2. Watson, R.L. et al. (2015) Endothelial CD99 signals through soluble adenylyl cyclase and PKA to regulate leukocyte transendothelial migration.
    J Exp Med. 212 (7): 1021-41.

Synonyms
ICAM-1
RRID
AB_10841631
UniProt
P05362
Entrez Gene
ICAM1
GO Terms
GO:0007157 heterophilic cell-cell adhesion
GO:0001910 regulation of leukocyte mediated cytotoxicity
GO:0002291 T cell activation via T cell receptor contact with antigen bound to MHC molecule on antigen presenting cell
GO:0002693 positive regulation of cellular extravasation
GO:0004888 transmembrane receptor activity
GO:0005887 integral to plasma membrane
GO:0005178 integrin binding
GO:0005615 extracellular space
GO:0007159 leukocyte cell-cell adhesion
GO:0022614 membrane to membrane docking
GO:0046813 virion attachment, binding of host cell surface receptor
GO:0050776 regulation of immune response
GO:0050900 leukocyte migration
GO:0051856 adhesion to symbiont
GO:0060333 interferon-gamma-mediated signaling pathway
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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