CD44v3-v10 antibody

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Goat anti Human CD44v3-v10

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
AHP1501 C P* 1 mg
Goat anti Human CD44v3-v10 antibody recognizes epitopes encoded by exons v3-v10 on the variant portion of human CD44.

CD44 is a ubiquitously expressed type 1 transmembrane glycoprotein and proteoglycan, otherwise known as phagocytic glycoprotein 1 (pgp 1), acting as a receptor for the major extracellular matrix component hyaluronate, and also binds other ligands including collagen and matrix metalloproteinases (Miletti-González et al. 2012).

CD44 plays a role in cell-cell interactions, cell adhesion, the activation and homing of lymphocytes, cell migration, haematopoiesis and metastasis, and exists as a number of isoforms generated by the alternative RNA splicing of at least 10 variant exons (Tanabe et al. 1993).

Product Details

Target Species
Human
Product Form
Purified IgG - liquid
Antiserum Preparation
Antiserum to human CD44v3-v10 was raised by repeated immunisation of goats with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Immunogen
Recombinant human CD44v3-v10
Approx. Protein Concentrations
IgG concentration 1.0mg/ml

Storage Information

Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.

More Information

UniProt
P16070 Related reagents
Entrez Gene
CD44 Related reagents
Regulatory
For research purposes only

Applications of CD44v3-v10 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Immunohistology - Frozen 50ug/ml
Immunohistology - Paraffin 1 20ug/ml
  1. 1This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.Sodium citrate buffer pH 6.0 is recommended for this purpose.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Histology Positive Control Tissue
Breast carcinoma and colon adenocarcinoma

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Rabbit anti Goat IgG (Fc):FITC STAR122F 1 mg F
Rabbit anti Goat IgG (Fc):HRP STAR122P 1 mg C E WB

Useful Reagents Available

Description Product Code Pack Size Applications List Price Quantity
Antigen Retrieval Buffer, pH8.0 BUF025A 500 ml P
Antigen Retrieval Buffer, pH8.0 BUF025C 50 ml P
Histar Detection System STAR3000B 150 Tests C P

Product Specific References

References for CD44v3-v10 antibody

  1. Zhang D et al. (2015) Screening and Identification of a Phage Display Derived Peptide That Specifically Binds to the CD44 Protein Region Encoded by Variable Exons.
    J Biomol Screen. pii: 1087057115608604.