CD318 antibody

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Anti Human CD318 (C-Terminal) Antibody gallery image 1 — Western blotting analysis of human colon lysate (35μg protein in RIPA buffer) probed with Goat anti Human CD318 antibody (**AHP1567**) at 1.0μgml^-1. Primary incubation was 1 hour. Signal was detected by chemiluminescence.

Goat anti Human CD318 (C-Terminal)

Product Type: Polyclonal Antibody
Isotype: Polyclonal IgG

Product Code	Applications	Pack Size	Your Price	List Price	Quantity
AHP1567 Datasheet	E WB MSDS	0.1 mg	Log in		Get Quote

Summary
Secondary Antibodies

Goat anti Human CD318 antibody recognises an epitope within the C-terminal (CT) region of isoform 1 of human CD318, otherwise known as CDCP1 (CUB domain-containing protein 1), a novel type I transmembrane protein expressed on the surface of haematopoietic stem cell subsets, epithelial cells and breast carcinoma, and up-regulated in metastatic lung and colorectal tumors.

Expression of CD318 on CD34⁺CD133⁺ leukaemic blasts from patients with AML (acute myeloid leukaemia) and CML (chronic myeloid leukaemia), suggesting a role for CD318 as a novel leukaemic diagnostic marker (Uhr 2013).

Product Details

Target Species: Human
Product Form: Purified IgG - liquid
Antiserum Preparation: Antiserum to human CD318 (CT) was raised by repeated immunisation of goats with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
Buffer Solution: TRIS buffered saline
Preservative Stabilisers: 0.02% Sodium Azide (NaN₃)
0.5% Bovine Serum Albumin
Immunogen: Peptide sequence C-PLLNTQEPMEPAE corresponding to the C-Terminal region of CD318 (NP_073753.3).
Approx. Protein Concentrations: IgG concentration 0.5mg/ml

Storage Information

Storage: Store at +4^oC or at -20^oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Guarantee: 12 months from date of despatch

More Information

UniProt: Q9H5V8
Entrez Gene: CDCP1
GO Terms: GO:0005886 plasma membrane; GO:0016021 integral to membrane; GO:0005576 extracellular region
Regulatory: For research purposes only

Applications of CD318 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Verified	Min Dilution	Max Dilution
ELISA			1/16000
Western Blotting		1.0	3.0ug/ml

Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

Western Blotting: AHP1567 detects a band of approximately 90kDa in human colon cell lysates.

Secondary Antibodies Available

Description	Product Code	Applications	Pack Size	Your Price	List Price	Quantity
Rabbit anti Goat IgG (Fc):FITC	STAR122F	F	1 mg	Log in		Get Quote
Rabbit anti Goat IgG (Fc):HRP	STAR122P	C E WB	1 mg	Log in		Get Quote

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