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CD318 antibody

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Anti Human CD318 (C-Terminal) Antibody gallery image 1
Western blotting analysis of human colon lysate (35μg protein in RIPA buffer) probed with Goat anti Human CD318 antibody (AHP1567) at 1.0μgml-1. Primary incubation was 1 hour. Signal was detected by chemiluminescence.

Goat anti Human CD318 (C-Terminal)

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Product Code Applications Pack Size List Price Quantity
AHP1567
Datasheet
E WB
SDS
0.1 mg

Goat anti Human CD318 antibody recognises an epitope within the C-terminal (CT) region of isoform 1 of human CD318, otherwise known as CDCP1 (CUB domain-containing protein 1), a novel type I transmembrane protein expressed on the surface of haematopoietic stem cell subsets, epithelial cells and breast carcinoma, and up-regulated in metastatic lung and colorectal tumors.

Expression of CD318 on CD34+CD133+ leukaemic blasts from patients with AML (acute myeloid leukaemia) and CML (chronic myeloid leukaemia), suggesting a role for CD318 as a novel leukaemic diagnostic marker (Uhr 2013).

Product Details

Target Species
Human
Product Form
Purified IgG - liquid
Antiserum Preparation
Antiserum to human CD318 (CT) was raised by repeated immunisation of goats with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
Buffer Solution
TRIS buffered saline
Preservative Stabilisers
0.02% Sodium Azide (NaN3)
0.5% Bovine Serum Albumin
Immunogen
Peptide sequence C-PLLNTQEPMEPAE corresponding to the C-Terminal region of CD318 (NP_073753.3).
Approx. Protein Concentrations
IgG concentration 0.5mg/ml

Storage Information

Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Guarantee
12 months from date of despatch

More Information

UniProt
Q9H5V8
Entrez Gene
CDCP1
GO Terms
GO:0005886 plasma membrane
GO:0016021 integral to membrane
GO:0005576 extracellular region
Regulatory
For research purposes only

Applications of CD318 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 1/16000
Western Blotting 1.0 3.0ug/ml
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Western Blotting
AHP1567 detects a band of approximately 90kDa in human colon cell lysates.
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Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Rabbit anti Goat IgG (Fc):FITC STAR122F F 1 mg loader
Rabbit anti Goat IgG (Fc):HRP STAR122P C E WB 1 mg loader

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