CD283 antibody

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Rabbit anti Human CD283

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
AHP1019 C WB 0.1 mg
Rabbit anti Human CD283 antibody recognizes human CD283, otherwise known as toll receptor 3, a 103.8 kDa member of the evolutionarily conserved toll-like receptor family (TLRs), expressed at high levels in the placenta and pancreas and also expressed by dendritic cells.

TLRs play an important role in the recognition of invading microbial pathogens during innate immunity, acting through adaptor molecules such as MyD88 and TRAF6. CD283 acts via a cytokine signaling pathway to induce the activation of NF-kappaB and type I interferons, and has been shown to recognize double-stranded viral RNA, including that of the Influenza A and West Nile virus.

Rabbit anti Human CD283 antibody has been shown as suitable for use in IHC on EL4 cytospins at 1ug/ml.

Product Details

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Antiserum Preparation
Antisera to human CD283 were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG was prepared by ion exchange chromatography.
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.02%Sodium Azide (NaN3)
Immunogen
Synthetic peptide corresponding to a 15 amino acid sequence located near human CD283 carboxy-terminus.
Approx. Protein Concentrations
IgG concentration 1.0mg/ml

Storage Information

Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.

More Information

UniProt
O15455 Related reagents
Entrez Gene
TLR3 Related reagents
GO Terms
GO:0002756 MyD88-independent toll-like receptor signaling pathway
GO:0003725 double-stranded RNA binding
GO:0042742 defense response to bacterium
GO:0004888 transmembrane receptor activity
GO:0005887 integral to plasma membrane
GO:0005789 endoplasmic reticulum membrane
GO:0006954 inflammatory response
GO:0006972 hyperosmotic response
GO:0007250 activation of NF-kappaB-inducing kinase activity
GO:0007252 I-kappaB phosphorylation
GO:0009597 detection of virus
GO:0010008 endosome membrane
GO:0010552 positive regulation of gene-specific transcription from RNA polymerase II promoter
GO:0032722 positive regulation of chemokine production
GO:0032728 positive regulation of interferon-beta production
GO:0032735 positive regulation of interleukin-12 production
GO:0032755 positive regulation of interleukin-6 production
GO:0032757 positive regulation of interleukin-8 production
GO:0032760 positive regulation of tumor necrosis factor production
GO:0034123 positive regulation of toll-like receptor signaling pathway
GO:0034138 toll-like receptor 3 signaling pathway
GO:0042346 positive regulation of NF-kappaB import into nucleus
GO:0045078 positive regulation of interferon-gamma biosynthetic process
GO:0045087 innate immune response
GO:0045356 positive regulation of interferon-alpha biosynthetic process
GO:0045359 positive regulation of interferon-beta biosynthetic process
GO:0045671 negative regulation of osteoclast differentiation
GO:0050729 positive regulation of inflammatory response
GO:0051607 defense response to virus
Regulatory
For research purposes only

Applications of CD283 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Immunohistology - Frozen
Western Blotting 1.0 2.0ug/ml
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Western Blotting
AHP1019 detects a band of approximately 103.8kDa in human Daudi cell lysates.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Sheep anti Rabbit IgG:Biotin 2AB02B 1 ml C E P WB
Goat anti Rabbit IgG (Fc):Biotin STAR121B 1 mg E WB
Goat anti Rabbit IgG (Fc):FITC STAR121F 1 mg F
Goat anti Rabbit IgG (Fc):HRP STAR121P 1 mg E WB
Goat anti Rabbit IgG (H/L):HRP STAR124P 1 mg C E WB
Sheep anti Rabbit IgG:FITC STAR34B 1 mg C F
Sheep anti Rabbit IgG:RPE STAR35A 1 ml F
Sheep anti Rabbit IgG:DyLight®488 STAR36D488GA 0.1 mg F IF WB
Sheep anti Rabbit IgG:DyLight®549 STAR36D549GA 0.1 mg F IF
Sheep anti Rabbit IgG:DyLight®649 STAR36D649GA 0.1 mg F IF
Sheep anti Rabbit IgG:DyLight®680 STAR36D680GA 0.1 mg F IF WB
Sheep anti Rabbit IgG:Dylight®800 STAR36D800GA 0.1 mg F IF WB

Useful Reagents Available

Description Product Code Pack Size Applications List Price Quantity
TidyBlot Western Blot Detection Reagent:HRP STAR209P 0.5 ml WB*

Product Specific References

Further Reading

  1. Alexopoulou, L. et al. (2001) Recognition of double-stranded RNA and activation of NF-kappaB by Toll-like receptor 3.
    Nature. 413 (6857): 732-8.
  2. Guillot, L. et al. (2005) Involvement of toll-like receptor 3 in the immune response of lung epithelial cells to double-stranded RNA and influenza A virus.
    J Biol Chem. 280 (7): 5571-80.
  3. Wang, T. et al. (2004) Toll-like receptor 3 mediates West Nile virus entry into the brain causing lethal encephalitis.
    Nat Med. 10 (12): 1366-73.