CD283 antibody
Rabbit anti Human CD283 antibody recognizes human CD283, otherwise known as toll receptor 3, a 103.8 kDa member of the evolutionarily conserved toll-like receptor family (TLRs), expressed at high levels in the placenta and pancreas and also expressed by dendritic cells.
TLRs play an important role in the recognition of invading microbial pathogens during innate immunity, acting through adaptor molecules such as MyD88 and TRAF6. CD283 acts via a cytokine signaling pathway to induce the activation of NF-kappaB and type I interferons, and has been shown to recognize double-stranded viral RNA, including that of the Influenza A and West Nile virus.
Rabbit anti Human CD283 antibody has been shown as suitable for use in IHC on EL4 cytospins at 1ug/ml.
TLRs play an important role in the recognition of invading microbial pathogens during innate immunity, acting through adaptor molecules such as MyD88 and TRAF6. CD283 acts via a cytokine signaling pathway to induce the activation of NF-kappaB and type I interferons, and has been shown to recognize double-stranded viral RNA, including that of the Influenza A and West Nile virus.
Rabbit anti Human CD283 antibody has been shown as suitable for use in IHC on EL4 cytospins at 1ug/ml.
Product Details
- Target Species
- Human
- Species Cross-Reactivity
-
Target Species Cross Reactivity Mouse - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Antiserum Preparation
- Antisera to human CD283 were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG was prepared by affinity chromatography.
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
0.02% Sodium Azide (NaN3) - Immunogen
- Synthetic peptide corresponding to a 15 amino acid sequence located near human CD283 carboxy-terminus.
- Approx. Protein Concentrations
- IgG concentration 1.0mg/ml
Storage Information
- Storage
- This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. - Guarantee
- 12 months from date of despatch
More Information
- UniProt
- O15455
- Entrez Gene
- TLR3
- GO Terms
- GO:0002756 MyD88-independent toll-like receptor signaling pathway
- GO:0003725 double-stranded RNA binding
- GO:0042742 defense response to bacterium
- GO:0004888 transmembrane receptor activity
- GO:0005887 integral to plasma membrane
- GO:0005789 endoplasmic reticulum membrane
- GO:0006954 inflammatory response
- GO:0006972 hyperosmotic response
- GO:0007250 activation of NF-kappaB-inducing kinase activity
- GO:0007252 I-kappaB phosphorylation
- GO:0009597 detection of virus
- GO:0010008 endosome membrane
- GO:0010552 positive regulation of gene-specific transcription from RNA polymerase II promoter
- GO:0032722 positive regulation of chemokine production
- GO:0032728 positive regulation of interferon-beta production
- GO:0032735 positive regulation of interleukin-12 production
- GO:0032755 positive regulation of interleukin-6 production
- GO:0032757 positive regulation of interleukin-8 production
- GO:0032760 positive regulation of tumor necrosis factor production
- GO:0034123 positive regulation of toll-like receptor signaling pathway
- GO:0034138 toll-like receptor 3 signaling pathway
- GO:0042346 positive regulation of NF-kappaB import into nucleus
- GO:0045078 positive regulation of interferon-gamma biosynthetic process
- GO:0045087 innate immune response
- GO:0045356 positive regulation of interferon-alpha biosynthetic process
- GO:0045359 positive regulation of interferon-beta biosynthetic process
- GO:0045671 negative regulation of osteoclast differentiation
- GO:0050729 positive regulation of inflammatory response
- GO:0051607 defense response to virus
- Regulatory
- For research purposes only
Applications of CD283 antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Immunohistology - Frozen | |||
| Western Blotting | 1.0 | 2.0ug/ml |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Western Blotting
- AHP1019 detects a band of approximately 103.8kDa in human Daudi cell lysates.
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Secondary Antibodies Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| Goat anti Rabbit IgG (Fc):Biotin | STAR121B | E WB | 1 mg |
|
|
| Goat anti Rabbit IgG (Fc):FITC | STAR121F | F | 1 mg |
|
|
| Goat anti Rabbit IgG (Fc):HRP | STAR121P | E WB | 1 mg |
|
|
| Goat anti Rabbit IgG (H/L):HRP | STAR124P | C E WB | 1 mg |
|
|
| Sheep anti Rabbit IgG:FITC | STAR34B | C F | 1 mg |
|
|
| Sheep anti Rabbit IgG:RPE | STAR35A | F | 1 ml |
|
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| Sheep anti Rabbit IgG:DyLight®488 | STAR36D488GA | F IF WB | 0.1 mg | ||
| Sheep anti Rabbit IgG:DyLight®680 | STAR36D680GA | F IF WB | 0.1 mg | ||
| Sheep anti Rabbit IgG:Dylight®800 | STAR36D800GA | F IF WB | 0.1 mg |
Useful Reagents Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| TidyBlot Western Blot Detection Reagent:HRP | STAR209P | WB* | 0.5 ml |
Product Specific References
Further Reading
-
Alexopoulou, L. et al. (2001) Recognition of double-stranded RNA and activation of NF-kappaB by Toll-like receptor 3.
Nature. 413 (6857): 732-8. -
Guillot, L. et al. (2005) Involvement of toll-like receptor 3 in the immune response of lung epithelial cells to double-stranded RNA and influenza A virus.
J Biol Chem. 280 (7): 5571-80. -
Wang, T. et al. (2004) Toll-like receptor 3 mediates West Nile virus entry into the brain causing lethal encephalitis.
Nat Med. 10 (12): 1366-73.
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