CD283 antibody

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Anti Human CD283 Antibody thumbnail image 2

Anti Human CD283 Antibody gallery image 1 — Western blot analysis of whole cell lysate from Daudi human Burkitt's lymphoma derived cells probed with Rabbit anti Human CD283 (AHP1019) at 1(A) and 2(B) ug/ml

Anti Human CD283 Antibody gallery image 2 — Immunocytochemical staining of EL4 mouse lymphoblast cells with Rabbit anti Human CD283 (AHP1019)

Rabbit anti Human CD283

Product Type: Polyclonal Antibody
Isotype: Polyclonal IgG

Product Code	Applications	Datasheet	MSDS	Pack Size	List Price	Quantity
AHP1019	C WB			0.1 mg

Rabbit anti Human CD283 antibody recognizes human CD283, otherwise known as toll receptor 3, a 103.8 kDa member of the evolutionarily conserved toll-like receptor family (TLRs), expressed at high levels in the placenta and pancreas and also expressed by dendritic cells.

TLRs play an important role in the recognition of invading microbial pathogens during innate immunity, acting through adaptor molecules such as MyD88 and TRAF6. CD283 acts via a cytokine signaling pathway to induce the activation of NF-kappaB and type I interferons, and has been shown to recognize double-stranded viral RNA, including that of the Influenza A and West Nile virus.

Rabbit anti Human CD283 antibody has been shown as suitable for use in IHC on EL4 cytospins at 1ug/ml.

Product Details

Target Species

Human

Species Cross-Reactivity

Target Species	Cross Reactivity
Mouse

N.B. Antibody reactivity and working conditions may vary between species.

Product Form

Purified IgG - liquid

Antiserum Preparation

Antisera to human CD283 were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG was prepared by ion exchange chromatography.

Buffer Solution

Phosphate buffered saline

Preservative Stabilisers

0.02%

Sodium Azide (NaN₃)

Immunogen

Synthetic peptide corresponding to a 15 amino acid sequence located near human CD283 carboxy-terminus.

Approx. Protein Concentrations

IgG concentration 1.0mg/ml

Storage Information

Storage: Store at +4^oC or at -20^oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life: 18 months from date of despatch.

More Information

UniProt: O15455 Related reagents
Entrez Gene: TLR3 Related reagents
GO Terms: GO:0002756 MyD88-independent toll-like receptor signaling pathway; GO:0003725 double-stranded RNA binding; GO:0042742 defense response to bacterium; GO:0004888 transmembrane receptor activity; GO:0005887 integral to plasma membrane; GO:0005789 endoplasmic reticulum membrane; GO:0006954 inflammatory response; GO:0006972 hyperosmotic response; GO:0007250 activation of NF-kappaB-inducing kinase activity; GO:0007252 I-kappaB phosphorylation; GO:0009597 detection of virus; GO:0010008 endosome membrane; GO:0010552 positive regulation of gene-specific transcription from RNA polymerase II promoter; GO:0032722 positive regulation of chemokine production; GO:0032728 positive regulation of interferon-beta production; GO:0032735 positive regulation of interleukin-12 production; GO:0032755 positive regulation of interleukin-6 production; GO:0032757 positive regulation of interleukin-8 production; GO:0032760 positive regulation of tumor necrosis factor production; GO:0034123 positive regulation of toll-like receptor signaling pathway; GO:0034138 toll-like receptor 3 signaling pathway; GO:0042346 positive regulation of NF-kappaB import into nucleus; GO:0045078 positive regulation of interferon-gamma biosynthetic process; GO:0045087 innate immune response; GO:0045356 positive regulation of interferon-alpha biosynthetic process; GO:0045359 positive regulation of interferon-beta biosynthetic process; GO:0045671 negative regulation of osteoclast differentiation; GO:0050729 positive regulation of inflammatory response; GO:0051607 defense response to virus
Regulatory: For research purposes only

Applications of CD283 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Verified	Min Dilution	Max Dilution
Immunohistology - Frozen
Western Blotting		1.0	2.0ug/ml

Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

Western Blotting: AHP1019 detects a band of approximately 103.8kDa in human Daudi cell lysates.

Secondary Antibodies Available

Description	Product Code	Pack Size	Applications
Sheep anti Rabbit IgG:Biotin	2AB02B	1 ml	C E P WB
Goat anti Rabbit IgG (Fc):Biotin	STAR121B	1 mg	E WB
Goat anti Rabbit IgG (Fc):FITC	STAR121F	1 mg	F
Goat anti Rabbit IgG (Fc):HRP	STAR121P	1 mg	E WB
Goat anti Rabbit IgG (H/L):HRP	STAR124P	1 mg	C E WB
Sheep anti Rabbit IgG:FITC	STAR34B	1 mg	C F
Sheep anti Rabbit IgG:RPE	STAR35A	1 ml	F
Sheep anti Rabbit IgG:DyLight®488	STAR36D488GA	0.1 mg	F IF WB
Sheep anti Rabbit IgG:DyLight®549	STAR36D549GA	0.1 mg	F IF
Sheep anti Rabbit IgG:DyLight®649	STAR36D649GA	0.1 mg	F IF
Sheep anti Rabbit IgG:DyLight®680	STAR36D680GA	0.1 mg	F IF WB
Sheep anti Rabbit IgG:Dylight®800	STAR36D800GA	0.1 mg	F IF WB

Useful Reagents Available

Description	Product Code	Pack Size	Applications	List Price	Quantity
TidyBlot™ Western Blot Detection Reagent:HRP	STAR209P	0.5 ml	WB*