CD283 antibody


TLRs play an important role in the recognition of invading microbial pathogens during innate immunity, acting through adaptor molecules such as MyD88 and TRAF6. CD283 acts via a cytokine signaling pathway to induce the activation of NF-kappaB and type I interferons, and has been shown to recognize double-stranded viral RNA, including that of the Influenza A and West Nile virus.
Rabbit anti Human CD283 antibody has been shown as suitable for use in IHC on EL4 cytospins at 1ug/ml.
Product Details
- Target Species
- Human
- Species Cross-Reactivity
-
Target Species Cross Reactivity Mouse - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Antiserum Preparation
- Antisera to human CD283 were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG was prepared by affinity chromatography.
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
0.02% Sodium Azide (NaN3) - Immunogen
- Synthetic peptide corresponding to a 15 amino acid sequence located near human CD283 carboxy-terminus.
- Approx. Protein Concentrations
- IgG concentration 1.0mg/ml
Storage Information
- Storage
- Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Guarantee
- 12 months from date of despatch
More Information
- UniProt
- O15455
- Entrez Gene
- TLR3
- GO Terms
- GO:0002756 MyD88-independent toll-like receptor signaling pathway
- GO:0003725 double-stranded RNA binding
- GO:0042742 defense response to bacterium
- GO:0004888 transmembrane receptor activity
- GO:0005887 integral to plasma membrane
- GO:0005789 endoplasmic reticulum membrane
- GO:0006954 inflammatory response
- GO:0006972 hyperosmotic response
- GO:0007250 activation of NF-kappaB-inducing kinase activity
- GO:0007252 I-kappaB phosphorylation
- GO:0009597 detection of virus
- GO:0010008 endosome membrane
- GO:0010552 positive regulation of gene-specific transcription from RNA polymerase II promoter
- GO:0032722 positive regulation of chemokine production
- GO:0032728 positive regulation of interferon-beta production
- GO:0032735 positive regulation of interleukin-12 production
- GO:0032755 positive regulation of interleukin-6 production
- GO:0032757 positive regulation of interleukin-8 production
- GO:0032760 positive regulation of tumor necrosis factor production
- GO:0034123 positive regulation of toll-like receptor signaling pathway
- GO:0034138 toll-like receptor 3 signaling pathway
- GO:0042346 positive regulation of NF-kappaB import into nucleus
- GO:0045078 positive regulation of interferon-gamma biosynthetic process
- GO:0045087 innate immune response
- GO:0045356 positive regulation of interferon-alpha biosynthetic process
- GO:0045359 positive regulation of interferon-beta biosynthetic process
- GO:0045671 negative regulation of osteoclast differentiation
- GO:0050729 positive regulation of inflammatory response
- GO:0051607 defense response to virus
- Regulatory
- For research purposes only
Applications of CD283 antibody
Application Name | Verified | Min Dilution | Max Dilution |
---|---|---|---|
Immunohistology - Frozen | |||
Western Blotting | 1.0 | 2.0ug/ml |
- Western Blotting
- AHP1019 detects a band of approximately 103.8kDa in human Daudi cell lysates.
Secondary Antibodies Available
Description | Product Code | Applications | Pack Size | List Price | Quantity |
---|---|---|---|---|---|
Goat anti Rabbit IgG (Fc):Biotin | STAR121B | E WB | 1 mg |
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Goat anti Rabbit IgG (Fc):FITC | STAR121F | F | 1 mg |
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Goat anti Rabbit IgG (Fc):HRP | STAR121P | E WB | 1 mg |
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|
Goat anti Rabbit IgG (H/L):HRP | STAR124P | C E WB | 1 mg |
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|
Sheep anti Rabbit IgG:FITC | STAR34B | C F | 1 mg |
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|
Sheep anti Rabbit IgG:RPE | STAR35A | F | 1 ml |
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|
Sheep anti Rabbit IgG:DyLight®488 | STAR36D488GA | F IF WB | 0.1 mg | ||
Sheep anti Rabbit IgG:DyLight®680 | STAR36D680GA | F IF WB | 0.1 mg | ||
Sheep anti Rabbit IgG:Dylight®800 | STAR36D800GA | F IF WB | 0.1 mg |
Useful Reagents Available
Description | Product Code | Applications | Pack Size | List Price | Quantity |
---|---|---|---|---|---|
TidyBlot Western Blot Detection Reagent:HRP | STAR209P | WB* | 0.5 ml |
Product Specific References
Further Reading
-
Alexopoulou, L. et al. (2001) Recognition of double-stranded RNA and activation of NF-kappaB by Toll-like receptor 3.
Nature. 413 (6857): 732-8. -
Guillot, L. et al. (2005) Involvement of toll-like receptor 3 in the immune response of lung epithelial cells to double-stranded RNA and influenza A virus.
J Biol Chem. 280 (7): 5571-80. -
Wang, T. et al. (2004) Toll-like receptor 3 mediates West Nile virus entry into the brain causing lethal encephalitis.
Nat Med. 10 (12): 1366-73.
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