Rabbit anti Human CD282 antibody recognises CD282, also known as TLR-2, a ~90 kDa type 1 transmembrane protein that is primarily expressed by peripheral blood monocytes. CD282 is also expressed in the spleen, bone marrow and lymph nodes. CD282 is reported to respond to a diverse range of bacterial cell wall components, mediating the innate immune response in co-operation with MD-2.
- Target Species
- Species Cross-Reactivity
|Target Species||Cross Reactivity|
- N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Antiserum Preparation
- Antisera to Human CD282 (NT) were raised by repeated immunisation of Rabbits with highly purified antigen. Purified IgG prepared from whole serum by ion exchange chromatography
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.02% Sodium Azide (NaN3)
- A peptide corresponding to an 14 amino acid sequence from near the amino terminus of human CD282.
- Approx. Protein Concentrations
- IgG concentration 1.0mg/ml
- Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
- 12 months from date of despatch
- Entrez Gene
- GO Terms
lipopolysaccharide receptor activity
MyD88-dependent toll-like receptor signaling pathway
transmembrane receptor activity
integral to plasma membrane
induction of apoptosis
Toll signaling pathway
positive regulation of gene-specific transcription from RNA polymerase II promoter
positive regulation of Wnt receptor signaling pathway
positive regulation of chemokine production
positive regulation of interferon-beta production
positive regulation of interleukin-12 production
positive regulation of interleukin-18 production
positive regulation of interleukin-6 production
positive regulation of interleukin-8 production
positive regulation of tumor necrosis factor production
positive regulation of toll-like receptor signaling pathway
toll-like receptor 1 signaling pathway
toll-like receptor 2 signaling pathway
toll-like receptor 4 signaling pathway
Toll-like receptor 1-Toll-like receptor 2 protein complex
positive regulation of NF-kappaB import into nucleus
detection of triacyl bacterial lipopeptide
detection of diacyl bacterial lipopeptide
triacyl lipopeptide binding
innate immune response
protein heterodimerization activity
positive regulation of inflammatory response
positive regulation of NF-kappaB transcription factor activity
Gram-positive bacterial cell surface binding
positive regulation of nitric-oxide synthase biosynthetic process
cellular response to lipoteichoic acid
cellular response to diacyl bacterial lipopeptide
cellular response to triacyl bacterial lipopeptide
- For research purposes only
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Applications of CD282 antibody
|Immunohistology - Paraffin 1
- 1This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.Sodium citrate buffer pH 6.0 is recommended for this purpose.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Histology Positive Control Tissue
- Human spleen
- Western Blotting
- AHP1635 detects a band of approximately 100kDa in A-20 cell lysate
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Secondary Antibodies Available
Useful Reagents Available
Product Specific References
References for CD282 antibody
Siemieniuch MJ et al. (2016) Type of Inflammation Differentially Affects Expression of Interleukin 1β and 6, Tumor Necrosis Factor-α and Toll-Like Receptors in Subclinical Endometritis in Mares.
PLoS One. 11 (5): e0154934.
Vogel, S.N. et al. (2003) TLRs: differential adapter utilization by toll-like receptors mediates TLR-specific patterns of gene expression.
Mol Interv. 3 (8): 466-77.
Takeda, K. et al. (2003) Toll-like receptors.
Annu Rev Immunol. 21: 335-76.
Janeway, C.A. Jr. & Medzhitov, R. (2002) Innate immune recognition.
Annu Rev Immunol. 20: 197-216.
O'Neill, L.A.J, et al (2003) The Toll-IL-1 receptor adaptor family grows to five members.
Trends in Imm. 24:286-9.
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