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CD282 antibody

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Anti Human CD282 (N-Terminal) Antibody thumbnail image 1 Anti Human CD282 (N-Terminal) Antibody thumbnail image 2 Anti Human CD282 (N-Terminal) Antibody thumbnail image 3
Anti Human CD282 (N-Terminal) Antibody gallery image 1
Western blot analysis of whole cell lysate from A20 mouse B cell lymphoma cell line probed with Rabbit anti Human CD282 (AHP1635)
Anti Human CD282 (N-Terminal) Antibody gallery image 2
Immunohistochemical staining of human spleen with Rabbit anti Human CD286 (AHP1635).
Anti Human CD282 (N-Terminal) Antibody gallery image 3
Published customer image:
Rabbit anti Human CD282 antibody used for the immunolocalization of TLR2 expression in equine endometrium by immunohistochemistry on formalin fixed, paraffin embedded tissue sections.
Image caption:
Immuno-localization of TNF-α (A, B), TLR2 (C, D) and TLR4 (E, F) in equine endometrial biopsies collected at estrus from control (A, C, E) or SE groups (B, D, F). LE-luminal epithelium; GE-glandular epithelium; S-endometrial stroma.

From: Siemieniuch MJ, Szóstek AZ, Gajos K, Kozdrowski R, Nowak M, Okuda K (2016)
Type of Inflammation Differentially Affects Expression of Interleukin 1β and 6, Tumor Necrosis Factor-α and Toll-Like Receptors in Subclinical Endometritis in Mares.
PLoS ONE 11(5): e0154934.

Rabbit anti Human CD282 (N-Terminal)

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Product Code Applications Pack Size List Price Quantity
AHP1635
Datasheet
P* WB
SDS
0.1 mg loader

Rabbit anti Human CD282 antibody recognises CD282, also known as TLR-2, a ~90 kDa type 1 transmembrane protein that is primarily expressed by peripheral blood monocytes. CD282 is also expressed in the spleen, bone marrow and lymph nodes. CD282 is reported to respond to a diverse range of bacterial cell wall components, mediating the innate immune response in co-operation with MD-2.

Product Details

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse
Horse
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Antiserum Preparation
Antisera to Human CD282 (NT) were raised by repeated immunisation of Rabbits with highly purified antigen. Purified IgG prepared from whole serum by ion exchange chromatography
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.02% Sodium Azide (NaN3)
Immunogen
A peptide corresponding to an 14 amino acid sequence from near the amino terminus of human CD282.
Approx. Protein Concentrations
IgG concentration 1.0mg/ml

Storage Information

Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Guarantee
12 months from date of despatch

More Information

UniProt
O60603
Entrez Gene
TLR2
GO Terms
GO:0001875 lipopolysaccharide receptor activity
GO:0002755 MyD88-dependent toll-like receptor signaling pathway
GO:0004888 transmembrane receptor activity
GO:0005887 integral to plasma membrane
GO:0005737 cytoplasm
GO:0006917 induction of apoptosis
GO:0006954 inflammatory response
GO:0007252 I-kappaB phosphorylation
GO:0008063 Toll signaling pathway
GO:0010552 positive regulation of gene-specific transcription from RNA polymerase II promoter
GO:0030177 positive regulation of Wnt receptor signaling pathway
GO:0032722 positive regulation of chemokine production
GO:0032728 positive regulation of interferon-beta production
GO:0032735 positive regulation of interleukin-12 production
GO:0032741 positive regulation of interleukin-18 production
GO:0032755 positive regulation of interleukin-6 production
GO:0032757 positive regulation of interleukin-8 production
GO:0032760 positive regulation of tumor necrosis factor production
GO:0034123 positive regulation of toll-like receptor signaling pathway
GO:0034130 toll-like receptor 1 signaling pathway
GO:0034134 toll-like receptor 2 signaling pathway
GO:0034142 toll-like receptor 4 signaling pathway
GO:0035354 Toll-like receptor 1-Toll-like receptor 2 protein complex
GO:0042346 positive regulation of NF-kappaB import into nucleus
GO:0042495 detection of triacyl bacterial lipopeptide
GO:0042496 detection of diacyl bacterial lipopeptide
GO:0042497 triacyl lipopeptide binding
GO:0042834 peptidoglycan binding
GO:0045087 innate immune response
GO:0046982 protein heterodimerization activity
GO:0050729 positive regulation of inflammatory response
GO:0051092 positive regulation of NF-kappaB transcription factor activity
GO:0051637 Gram-positive bacterial cell surface binding
GO:0051770 positive regulation of nitric-oxide synthase biosynthetic process
GO:0071223 cellular response to lipoteichoic acid
GO:0071726 cellular response to diacyl bacterial lipopeptide
GO:0071727 cellular response to triacyl bacterial lipopeptide
Regulatory
For research purposes only

Applications of CD282 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Immunohistology - Paraffin 1 2.0ug
Western Blotting 1.0ug/ml
  1. 1This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.Sodium citrate buffer pH 6.0 is recommended for this purpose.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Histology Positive Control Tissue
Human spleen
Western Blotting
AHP1635 detects a band of approximately 100kDa in A-20 cell lysate
Copyright © 2021 Bio-Rad Antibodies (formerly AbD Serotec)

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Goat anti Rabbit IgG (Fc):Biotin STAR121B E WB 1 mg loader
Goat anti Rabbit IgG (Fc):FITC STAR121F F 1 mg loader
Goat anti Rabbit IgG (Fc):HRP STAR121P E WB 1 mg loader
Goat anti Rabbit IgG (H/L):HRP STAR124P C E WB 1 mg loader
Sheep anti Rabbit IgG:FITC STAR34B C F 1 mg loader
Sheep anti Rabbit IgG:RPE STAR35A F 1 ml loader
Sheep anti Rabbit IgG:DyLight®488 STAR36D488GA F IF WB 0.1 mg
Sheep anti Rabbit IgG:DyLight®680 STAR36D680GA F IF WB 0.1 mg
Sheep anti Rabbit IgG:Dylight®800 STAR36D800GA F IF WB 0.1 mg

Useful Reagents Available

Description Product Code Applications Pack Size List Price Quantity
Antigen Retrieval Buffer, pH8.0 BUF025A P 500 ml
Antigen Retrieval Buffer, pH8.0 BUF025C P 50 ml
TidyBlot Western Blot Detection Reagent:HRP STAR209P WB* 0.5 ml

Product Specific References

References for CD282 antibody

  1. Siemieniuch MJ et al. (2016) Type of Inflammation Differentially Affects Expression of Interleukin 1β and 6, Tumor Necrosis Factor-α and Toll-Like Receptors in Subclinical Endometritis in Mares.
    PLoS One. 11 (5): e0154934.

Further Reading

  1. Vogel, S.N. et al. (2003) TLRs: differential adapter utilization by toll-like receptors mediates TLR-specific patterns of gene expression.
    Mol Interv. 3 (8): 466-77.
  2. Takeda, K. et al. (2003) Toll-like receptors.
    Annu Rev Immunol. 21: 335-76.
  3. Janeway, C.A. Jr. & Medzhitov, R. (2002) Innate immune recognition.
    Annu Rev Immunol. 20: 197-216.
  4. O'Neill, L.A.J, et al (2003) The Toll-IL-1 receptor adaptor family grows to five members.
    Trends in Imm. 24:286-9.

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