CD282 antibody

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Anti Human CD282 (N-Terminal) Antibody thumbnail image 1

Anti Human CD282 (N-Terminal) Antibody thumbnail image 2

Anti Human CD282 (N-Terminal) Antibody thumbnail image 3

Anti Human CD282 (N-Terminal) Antibody gallery image 1 — Western blot analysis of whole cell lysate from A20 mouse B cell lymphoma cell line probed with Rabbit anti Human CD282 (**AHP1635**)

Anti Human CD282 (N-Terminal) Antibody gallery image 2 — Immunohistochemical staining of human spleen with Rabbit anti Human CD286 (**AHP1635**).

Anti Human CD282 (N-Terminal) Antibody gallery image 3 — **Published customer image:**
Rabbit anti Human CD282 antibody (**AHP1635**) used for the immunolocalization of TLR2 expression in equine endometrium by immunohistochemistry on formalin fixed, paraffin embedded tissue sections.
**Image caption:**
Immuno-localization of TNF-α (A, B), TLR2 (C, D) and TLR4 (E, F) in equine endometrial biopsies collected at estrus from control (A, C, E) or SE groups (B, D, F). LE-luminal epithelium; GE-glandular epithelium; S-endometrial stroma.

From: Siemieniuch MJ, Szóstek AZ, Gajos K, Kozdrowski R, Nowak M, Okuda K (2016)
Type of Inflammation Differentially Affects Expression of Interleukin 1β and 6, Tumor Necrosis Factor-α and Toll-Like Receptors in Subclinical Endometritis in Mares.
PLoS ONE 11(5): e0154934.
doi: 10.1371/journal.pone.0154934.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.

Rabbit anti Human CD282 (N-Terminal)

Product Type: Polyclonal Antibody
Isotype: Polyclonal IgG

Product Code	Applications	Pack Size	Your Price	List Price	Quantity
AHP1635 Datasheet	P* WB SDS	0.1 mg	Log in		Get Quote

Rabbit anti Human CD282 antibody recognises CD282, also known as TLR-2, a ~90 kDa type 1 transmembrane protein that is primarily expressed by peripheral blood monocytes. CD282 is also expressed in the spleen, bone marrow and lymph nodes. CD282 is reported to respond to a diverse range of bacterial cell wall components, mediating the innate immune response in co-operation with MD-2.

Product Details

Target Species

Human

Species Cross-Reactivity

Target Species	Cross Reactivity
Mouse
Horse

N.B. Antibody reactivity and working conditions may vary between species.

Product Form

Purified IgG - liquid

Antiserum Preparation

Antisera to Human CD282 (NT) were raised by repeated immunisation of Rabbits with highly purified antigen. Purified IgG prepared from whole serum by ion exchange chromatography

Buffer Solution

Phosphate buffered saline

Preservative Stabilisers

0.02% Sodium Azide (NaN₃)

Immunogen

A peptide corresponding to an 14 amino acid sequence from near the amino terminus of human CD282.

Approx. Protein Concentrations

IgG concentration 1.0mg/ml

Storage Information

Storage: This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Guarantee: 12 months from date of despatch

More Information

UniProt: O60603
Entrez Gene: TLR2
GO Terms: GO:0001875 lipopolysaccharide receptor activity; GO:0002755 MyD88-dependent toll-like receptor signaling pathway; GO:0004888 transmembrane receptor activity; GO:0005887 integral to plasma membrane; GO:0005737 cytoplasm; GO:0006917 induction of apoptosis; GO:0006954 inflammatory response; GO:0007252 I-kappaB phosphorylation; GO:0008063 Toll signaling pathway; GO:0010552 positive regulation of gene-specific transcription from RNA polymerase II promoter; GO:0030177 positive regulation of Wnt receptor signaling pathway; GO:0032722 positive regulation of chemokine production; GO:0032728 positive regulation of interferon-beta production; GO:0032735 positive regulation of interleukin-12 production; GO:0032741 positive regulation of interleukin-18 production; GO:0032755 positive regulation of interleukin-6 production; GO:0032757 positive regulation of interleukin-8 production; GO:0032760 positive regulation of tumor necrosis factor production; GO:0034123 positive regulation of toll-like receptor signaling pathway; GO:0034130 toll-like receptor 1 signaling pathway; GO:0034134 toll-like receptor 2 signaling pathway; GO:0034142 toll-like receptor 4 signaling pathway; GO:0035354 Toll-like receptor 1-Toll-like receptor 2 protein complex; GO:0042346 positive regulation of NF-kappaB import into nucleus; GO:0042495 detection of triacyl bacterial lipopeptide; GO:0042496 detection of diacyl bacterial lipopeptide; GO:0042497 triacyl lipopeptide binding; GO:0042834 peptidoglycan binding; GO:0045087 innate immune response; GO:0046982 protein heterodimerization activity; GO:0050729 positive regulation of inflammatory response; GO:0051092 positive regulation of NF-kappaB transcription factor activity; GO:0051637 Gram-positive bacterial cell surface binding; GO:0051770 positive regulation of nitric-oxide synthase biosynthetic process; GO:0071223 cellular response to lipoteichoic acid; GO:0071726 cellular response to diacyl bacterial lipopeptide; GO:0071727 cellular response to triacyl bacterial lipopeptide
Regulatory: For research purposes only

Applications of CD282 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Verified	Min Dilution	Max Dilution
Immunohistology - Paraffin ¹			2.0ug
Western Blotting			1.0ug/ml

¹This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.Sodium citrate buffer pH 6.0 is recommended for this purpose.

Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

Histology Positive Control Tissue: Human spleen
Western Blotting: AHP1635 detects a band of approximately 100kDa in A-20 cell lysate

Secondary Antibodies Available

Description	Product Code	Applications	Pack Size	Your Price	Quantity
Goat anti Rabbit IgG (Fc):Biotin	STAR121B	E WB	1 mg	Log in	Get Quote
Goat anti Rabbit IgG (Fc):FITC	STAR121F	F	1 mg	Log in	Get Quote
Goat anti Rabbit IgG (Fc):HRP	STAR121P	E WB	1 mg	Log in	Get Quote
Goat anti Rabbit IgG (H/L):HRP	STAR124P	C E WB	1 mg	Log in	Get Quote
Sheep anti Rabbit IgG:FITC	STAR34B	C F	1 mg	Log in	Get Quote
Sheep anti Rabbit IgG:RPE	STAR35A	F	1 ml	Log in	Get Quote
Sheep anti Rabbit IgG:DyLight®488	STAR36D488GA	F IF WB	0.1 mg	Log in	Get Quote
Sheep anti Rabbit IgG:DyLight®680	STAR36D680GA	F IF WB	0.1 mg	Log in	Get Quote
Sheep anti Rabbit IgG:Dylight®800	STAR36D800GA	F IF WB	0.1 mg	Log in	Get Quote

Useful Reagents Available

Description	Product Code	Applications	Pack Size	Your Price	Quantity
Antigen Retrieval Buffer, pH8.0	BUF025A	P	500 ml	Log in	Get Quote
100x Antigen Retrieval Buffer, pH8.0	BUF025C	P	50 ml	Log in	Get Quote
TidyBlot Western Blot Detection Reagent:HRP	STAR209P	WB*	0.5 ml	Log in	Get Quote

Product Specific References

References for CD282 antibody

Siemieniuch MJ et al. (2016) Type of Inflammation Differentially Affects Expression of Interleukin 1β and 6, Tumor Necrosis Factor-α and Toll-Like Receptors in Subclinical Endometritis in Mares.
PLoS One. 11 (5): e0154934.

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